Substrates specialization in lipid compounds and hydrocarbons of Marinobacter genus

Bonin, Patricia; Vieira, Christophe; Grimaud, Régis; Militon, Cécile; Cuny, Philippe; Lima, Oscar; Guasco, Sophie; Brussaard, Corina P. D.; Michotey, Valérie

doi:10.1007/s11356-014-4009-y

Cited by 41 publications

(19 citation statements)

References 65 publications

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“…However, to the best of our knowledge, there have been no reports on the characterization of surface-active molecules production from the Marinobacter and Thalassospira although bacteria within these genera have been often retrieved from hydrocarbon-enriched marine communities [ 35 – 37 ]. It is also of note that, we were able to obtain bacteria mainly from the Marinobacter genus starting from sediments subjected to hydrocarbons contamination in the harbors, supporting previous studies reporting on the specialization of bacteria from this genus in lipids and hydrocarbons metabolism [ 29 ].…”

Section: Resultssupporting

confidence: 87%

Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills

et al. 2017

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BackgroundThe application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, stable and effective under marine environment context. Biosurfactants from marine bacteria could be good candidates for the development of biodispersant formulations effective in marine environment. This study aimed at establishing a collection of marine bacteria able to produce surface-active compounds and evaluating the activity and stability of the produced compounds under conditions mimicking those found under marine environment context.ResultsA total of 43 different isolates were obtained from harbor sediments. Twenty-six of them produced mainly bioemulsifiers when glucose was used as carbon source and 16 were biosurfactant/bioemulsifiers producers after growth in the presence of soybean oil. Sequencing of 16S rRNA gene classified most isolates into the genus Marinobacter. The produced emulsions were shown to be stable up to 30 months monitoring period, in the presence of 300 g/l NaCl, at 4 °C and after high temperature treatment (120 °C for 20 min). The partially purified compounds obtained after growth on soybean oil-based media exhibited low toxicity towards V. fischeri and high capability to disperse crude oil on synthetic marine water.ConclusionsTo the best of our knowledge, stability characterization of bioemulsifiers/biosurfactants from the non-pathogenic marine bacterium Marinobacter has not been previously reported. The produced compounds were shown to have potential for different applications including the environmental sector. Indeed, their high stability in the presence of high salt concentration and low temperature, conditions characterizing the marine environment, the capability to disperse crude oil and the low ecotoxicity makes them interesting for the development of biodispersants to be used in combatting marine oil spills.Electronic supplementary materialThe online version of this article (10.1186/s12934-017-0797-3) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 87%

Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills

et al. 2017

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“…Brevundimonas, Staphylococcus, Psychrobacter and Marinobacter all include PHs degrading species. The first two are often associated with PAH degradation [65,66], Psychrobacter is known to degrade alkanes [67] and Marinobacter has been reported to degrade both types of PHs [61]. BSF production under aerobic conditions was also observed in these four genera [68][69][70][71].…”

Section: Isolation and Identification Of Isolatesmentioning

confidence: 96%

“…Genera Bacillus, Brevundimonas, Psychrobacter, Staphylococcus, Marinobacter and Curtobacterium were also represented ( Table 6). All these genera include facultative anaerobes [59][60][61]. Bacillus includes some of the most studied BSF-producers under either aerobic or anaerobic conditions, some of them able to degrade PHs [62].…”

Section: Isolation and Identification Of Isolatesmentioning

confidence: 99%

Biosurfactant Production in Sub-Oxic Conditions Detected in Hydrocarbon-Degrading Isolates from Marine and Estuarine Sediments

Domingues

Oliveira

Serafim

et al. 2020

IJERPH

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Hydrocarbon bioremediation in anoxic sediment layers is still challenging not only because it involves metabolic pathways with lower energy yields but also because the production of biosurfactants that contribute to the dispersion of the pollutant is limited by oxygen availability. This work aims at screening populations of culturable hydrocarbonoclastic and biosurfactant (BSF) producing bacteria from deep sub-seafloor sediments (mud volcanos from Gulf of Cadiz) and estuarine sub-surface sediments (Ria de Aveiro) for strains with potential to operate in sub-oxic conditions. Isolates were retrieved from anaerobic selective cultures in which crude oil was provided as sole carbon source and different supplements were provided as electron acceptors. Twelve representative isolates were obtained from selective cultures with deep-sea and estuary sediments, six from each. These were identified by sequencing of 16S rRNA gene fragments belonging to Pseudomonas, Bacillus, Ochrobactrum, Brevundimonas, Psychrobacter, Staphylococcus, Marinobacter and Curtobacterium genera. BSF production by the isolates was tested by atomized oil assay, surface tension measurement and determination of the emulsification index. All isolates were able to produce BSFs under aerobic and anaerobic conditions, except for isolate DS27 which only produced BSF under aerobic conditions. These isolates presented potential to be applied in bioremediation or microbial enhanced oil recovery strategies under conditions of oxygen limitation. For the first time, members of Ochrobactrum, Brevundimonas, Psychrobacter, Staphylococcus, Marinobacter and Curtobacterium genera are described as anaerobic producers of BSFs. microorganisms have been identified as capable of producing BSF in anaerobiosis, with most of these belonging to the Bacillus and Pseudomonas genera [8].Hydrocarbonoclastic bacteria are characterized as being able to metabolize one or more PHs by using them as carbon and energy sources, usually preferring these substrates over others [9][10][11]. These bacteria are ubiquitous and can be found both in aerobic and anaerobic environments [12][13][14]. Natural anaerobic biodegradation of PHs occurs primarily in deep subsurface oil reservoirs [15], in the deep ocean near natural seeps [16,17] and in most microaerobic or anaerobic environments contaminated with PHs [18][19][20].While most of the deep-sea is characterized by low energy, productivity and biological activity rates [21], metabolic activity and biomass is higher in locations where seepage of PHs and other compounds occurs from within deep sediment layers to the seabed, such as hydrothermal vents and cold seeps [22,23]. Cold seeps, including mud volcanoes (MVs), are geologically diverse ecosystems associated with emissions of methane, other PHs and, often, reduced sulfate from the subsurface [24]. Deep-sea sediments, which are often anoxic below a thin upper layer [25], are known sinks for aliphatic and aromatic hydrocarbons [26,27]. Furthermore, bacterial communities from deep-sea surface sedi...

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“…In this context, approaches for incorporating genomics into microbial systematics are being developed and will become indispensable in the near future, with the sequencing of complete genomes of all type strains as the most critical challenge ( 2 – 4 ). In contrast, members of the genus Marinobacter , including Marinobacter flavimaris , are halophilic, hydrocarbonoclastic, and diazotrophic bacteria and are therefore especially useful for the bioremediation of hydrocarbon contaminants in both hypersaline environments and environments poor in nitrogenous compounds ( 5 – 7 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Marinobacter flavimaris LMG 23834 ^T , Which Is Potentially Useful in Bioremediation

et al. 2018

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Substrates specialization in lipid compounds and hydrocarbons of Marinobacter genus

Cited by 41 publications

References 65 publications

Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills

Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills

Biosurfactant Production in Sub-Oxic Conditions Detected in Hydrocarbon-Degrading Isolates from Marine and Estuarine Sediments

Complete Genome Sequence of Marinobacter flavimaris LMG 23834 ^T , Which Is Potentially Useful in Bioremediation

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Substrates specialization in lipid compounds and hydrocarbons of Marinobacter genus

Cited by 41 publications

References 65 publications

Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills

Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills

Biosurfactant Production in Sub-Oxic Conditions Detected in Hydrocarbon-Degrading Isolates from Marine and Estuarine Sediments

Complete Genome Sequence of Marinobacter flavimaris LMG 23834 T , Which Is Potentially Useful in Bioremediation

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Complete Genome Sequence of Marinobacter flavimaris LMG 23834 ^T , Which Is Potentially Useful in Bioremediation