Substrates of Nuclear Protein Kinases in Rat C6 Glial Cell Cultures

Jungmann, Richard A.; Harrison, Janie J.; Milkowski, Deborah M.; Lee, Seung-Ki; Schweppe, John S.; Miles, Michael F.

doi:10.1016/s0079-6123(08)63774-7

Cited by 5 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation that mRNA species are controlled by changes in both transcription and degradation rates has been made with several other cell systems (4,20,21,29). While many reasons could be advanced for multifaceted control of mRNA levels, in the specific case of procollagen mRNA and tendon cells, a rationale can be formulated as follows: In tendon tissue there is a need to control over the course of development the level of procollagen expression from less than 1% to about 50% of total protein synthesis (9).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA

1984

View full text Add to dashboard Cite

Procollagen alpha 2 (I) mRNA can be induced congruent to 6-fold in primary avian tendon (PAT) cells on addition of ascorbate to the culture medium. Previously, we have shown that the induction is linear after a 12 h lag and requires a total of 60-72 h to achieve maximum levels. We have now investigated in more detail the changes that have occurred in the metabolism of procollagen mRNA in fully induced cells to account for the observed induction. Ascorbate was found to triple the rate of procollagen gene transcription. In addition, there was a stabilization of the mRNA causing the half-life to increase from 10.5 h to 20 h. The increased stability of the procollagen mRNA, however, did not correlate with its ability to bind to oligo (dT)-cellulose. Since a 3-fold change in transcription rates and a 2-fold increase in half-life would account for the 6-fold overall increase in procollagen mRNA levels, we conclude that these are the primary alterations caused by ascorbate addition that give rise to the specific increase in procollagen mRNA.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Changes in specific degradation rates have been shown to be an important part of the regulation of mRNA levels in other cell systems (15,20,21,29,31). Therefore, in ascorbate induction of PAT cells part of the increase in procollagen mRNA levels could be due to an increase in the half-life of procollagen mRNA.…”

Section: Degradation Ratesmentioning

confidence: 99%

Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA

1984

View full text Add to dashboard Cite

show abstract

“…Furthermore, it has been known for several years that in C6 cells isozyme activity increases in response to 13-adrenergic receptor stimulation by catecholamines via a cAMP-mediated mechanism (3,4). Subsequent investigations into the mechanism of cAMP action have revealed that transcriptional as well as posttranscriptional effects are instrumental in the induction of the LDH A subunit (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Glucocorticoid induction of CRE-binding protein isoform mRNAs in rat C6 glioma cells

et al. 1992

Self Cite

View full text Add to dashboard Cite

Mammalian cells express several distinct isoforms of transcription factor CREB (cAMP-responsive element binding protein). At least two forms, alpha- and delta CREB, arise through alternative splicing of the CREB gene transcript. In this communication we demonstrate that the mRNAs of several CREB isoforms are expressed in rat C6 glioma cells and that the intracellular levels of these mRNAs are markedly induced by the synthetic glucocorticoid dexamethasone. Nuclear run-off assays show that the induction occurs, at least in part, through a transcriptional mechanism. The enhanced cellular levels of CREB mRNAs are accompanied by increased CREB protein and CRE-binding activity of nuclear extracts as evaluated by immunoblot and Southwestern blot assays.

show abstract

Induction of Myelin Components: Cyclic AMP Increases the Synthesis Rate of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in C₆ Glioma Cells

McMorris¹,

Smith²,

Sprinkle³

et al. 1985

Journal of Neurochemistry

View full text Add to dashboard Cite

In an effort to determine the factors that stimulate myelin synthesis, we investigated the mechanism by which dibutyryl cyclic AMP induces the activity of the myelin enzyme, 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP; EC 3.1.4.37), in C6 glioma cells. Immunotitration experiments and measurements of the accumulation of [35S]methionine-labeled CNP showed that dibutyryl cyclic AMP increased the amount of CNP in the cells but not the catalytic activity per molecule of the enzyme. Moreover, inhibition of protein synthesis with cycloheximide abolished induction of enzyme activity. Dibutyryl cyclic AMP doubled the rate of CNP synthesis but had no effect on the half-life of the enzyme (approximately 33 h). The induction was partially blocked by the inhibitors of mRNA synthesis, cordycepin or alpha-amanitin. Thus, cyclic AMP induces the synthesis of CNP.

show abstract

Substrates of Nuclear Protein Kinases in Rat C6 Glial Cell Cultures

Cited by 5 publications

References 39 publications

Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA

Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA

Glucocorticoid induction of CRE-binding protein isoform mRNAs in rat C6 glioma cells

Induction of Myelin Components: Cyclic AMP Increases the Synthesis Rate of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in C₆ Glioma Cells

Contact Info

Product

Resources

About

Substrates of Nuclear Protein Kinases in Rat C6 Glial Cell Cultures

Cited by 5 publications

References 39 publications

Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA

Ascorbate stimulation of PAT cells causes an increase in transcription rates and a decrease in degradation rates of procollagen mRNA

Glucocorticoid induction of CRE-binding protein isoform mRNAs in rat C6 glioma cells

Induction of Myelin Components: Cyclic AMP Increases the Synthesis Rate of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in C6 Glioma Cells

Contact Info

Product

Resources

About

Induction of Myelin Components: Cyclic AMP Increases the Synthesis Rate of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in C₆ Glioma Cells