Substrates for cardiovascular tissue engineering

Bouten, Cvc Carlijn; Dankers, Pyw Patricia; Driessen-Mol, Anita Anita; Pedrón, Sara; Brizard, AM; Baaijens, Fpt Frank

doi:10.1016/j.addr.2011.01.007

Cited by 231 publications

(206 citation statements)

References 309 publications

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“…Hydrogel degradation is most often characterized in vitro or by histological staining of excised in vivo samples 36,37 because tracking hydrogels in the heart is extremely difficult because of the dynamic nature of the beating heart, the depth of the heart within the animal, and the small volume of material injected therein (≈20-200 μL). 36,38 The near-infrared dye DyLight800 and the Kodak In-Vivo FX Pro Imaging System were used in this study to provide online information on hydrogel localization and lifespan.…”

Section: Discussionmentioning

confidence: 99%

Hydrogels With Integrin-Binding Angiopoietin-1–Derived Peptide, QHREDGS, for Treatment of Acute Myocardial Infarction

Reis

Chiu

et al. 2015

Circ: Heart Failure

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Chitosan and collagen are natural, biodegradable, and biocompatible polymers that have been explored for their potential use in the treatment of cardiac dysfunction. [4][5][6][7][8][9] Typically, collagen and chitosan, alone or in combination, are crosslinked using exogenous, sometimes toxic, chemical crosslinkers to improve the hydrogel mechanical properties. 4,10,11 However, we have previously shown that chitosan-collagen composites gel because of ionic interactions at physiological temperature and pH to form mechanically stable hydrogels that are appropriate for in vivo application.12 Furthermore, the collagen-chitosan interaction within the gels resembles the collagen-glycosaminoglycan interaction found in vivo in the extracellular matrix. 13 Thus, chitosan-collagen may mediate physiological cell-matrix interactions.The functional success of hydrogel-based cardiac and cell therapies can be improved by modifying biomaterials with bioactive molecules because bioactive molecules (cytokines, Original Article© 2015 American Heart Association, Inc.Circ Heart Fail is available at http://circheartfailure.ahajournals.org DOI: 10.1161/CIRCHEARTFAILURE.114.001881Background-Hydrogels are being actively investigated for direct delivery of cells or bioactive molecules to the heart after myocardial infarction (MI) to prevent cardiac functional loss. We postulate that immobilization of the prosurvival angiopoietin-1-derived peptide, QHREDGS, to a chitosan-collagen hydrogel could produce a clinically translatable thermoresponsive hydrogel to attenuate post-MI cardiac remodeling. Methods and Results-In a rat MI model, QHREDGS-conjugated hydrogel (QHG213H), control gel, or PBS was injected into the peri-infarct/MI zone. By in vivo tracking and chitosan staining, the hydrogel was demonstrated to remain in situ for 2 weeks and was cleared in ≈3 weeks. By echocardiography and pressure-volume analysis, the QHG213H hydrogel significantly improved cardiac function compared with the controls. Scar thickness and scar area fraction were also significantly improved with QHG213H gel injection compared with the controls. There were significantly more cardiomyocytes, determined by cardiac troponin-T staining, in the MI zone of the QHG213H hydrogel group; and hydrogel injection did not induce a significant inflammatory response as assessed by polymerase chain reaction and an inflammatory cytokine assay. The interaction of cardiomyocytes and cardiac fibroblasts with QHREDGS was found to be mediated by β 1 -integrins. Conclusions-We demonstrated for the first time that the QHG213H peptide-modified hydrogel can be injected in the beating heart where it remains localized for a clinically effective period. Moreover, the QHG213H hydrogel induced significant cardiac functional and morphological improvements after MI relative to the controls. and activates prosurvival pathways. 20 We identified the short sequence QHREDGS as the integrin-binding motif of Ang1, and the QHREDGS peptide was found to support cardiomyocyte attachment and survival simila...

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Section: Discussionmentioning

confidence: 99%

Hydrogels With Integrin-Binding Angiopoietin-1–Derived Peptide, QHREDGS, for Treatment of Acute Myocardial Infarction

Reis

Chiu

et al. 2015

Circ: Heart Failure

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“…hematopoiesis). In addition, developmental aspects, such as growth, differentiation, migration and maturation should be taken into account, as these may dictate substrate properties, like degradation and porosity, or require additional instrumentation of bioactive factors (Bouten et al, 2011). Such scaffolds with a possible application for in bone injection have to be designed and studied very carefully, because the progenitor cells derived from bone marrow may also be homed on to the bioactive substrate.…”

Section: Resultsmentioning

confidence: 99%

Osteoporosis: A Look at the Future

Kolev¹,

Иванова²,

Markova³

et al. 2012

Osteoporosis

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“…In these situations cardiac and peripheral bypass surgeries are necessary, requiring the replacement of a segment of blood vessels. The currently available options for these transplants are autologous grafts, allografts, xenografts and synthetic vascular grafts (16). The use of autografts and allografts is limited due to the lack of tissue donors, previous harvesting or anatomical variability (17).…”

Section: Discussionmentioning

confidence: 99%