Substrate topography interacts with substrate stiffness and culture time to regulate mechanical properties and smooth muscle differentiation of mesenchymal stem cells

Parandakh, Azim; Anbarlou, Azadeh; Tafazzoli-Shadpour, Mohammad; Ardeshirylajimi, Abdolreza; Khani, Mohammad‐Mehdi

doi:10.1016/j.colsurfb.2018.09.066

Cited by 29 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The materials with these topographic patterns act as anchoring sites for the adhesion of proteins present in the cell membranes and induce changes in cell size and shape, differentiation, and physiological phenotype [10,11,27,46,47]. Among several polymer-based materials, PDMS, PMMA, and poly(lactide-co-glycolide) (PLGA) were used to prepare distinct surface patterns with varying degrees of stiffness to understand the mechanisms of cellular interactions with materials [48]. The use of fibronectin-coated PDMS substrates that were engineered with anisotropic nanogratings and isotropic nanopillars of different dimensions helped to find the effects of nanotopographies on normal human lung fibroblasts (NHLFs) [49].…”

Section: Engineered Materialsmentioning

confidence: 99%

Molecular-Level Interactions between Engineered Materials and Cells

Jang

Jin

Shankar

et al. 2019

IJMS

View full text Add to dashboard Cite

Various recent experimental observations indicate that growing cells on engineered materials can alter their physiology, function, and fate. This finding suggests that better molecular-level understanding of the interactions between cells and materials may guide the design and construction of sophisticated artificial substrates, potentially enabling control of cells for use in various biomedical applications. In this review, we introduce recent research results that shed light on molecular events and mechanisms involved in the interactions between cells and materials. We discuss the development of materials with distinct physical, chemical, and biological features, cellular sensing of the engineered materials, transfer of the sensing information to the cell nucleus, subsequent changes in physical and chemical states of genomic DNA, and finally the resulting cellular behavior changes. Ongoing efforts to advance materials engineering and the cell–material interface will eventually expand the cell-based applications in therapies and tissue regenerations.

show abstract

Section: Engineered Materialsmentioning

confidence: 99%

Molecular-Level Interactions between Engineered Materials and Cells

Jang

Jin

Shankar

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…193 A strong interdependence of all properties might be the reason, why studies systematically investigating the effect of altered topography and mechanical characteristics are rare. [216][217][218][219][220][221][222]…”

Section: Materials Functionalization With Various Biochemical Groupsmentioning

confidence: 99%

Multifunctional Biomaterials: Combining Material Modification Strategies for Engineering of Cell-Contacting Surfaces

Mertgen

Trossmann

Guex

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

In the human body, cells in a tissue are exposed to signals derived from their specific extracellular matrix (ECM) cues from architectural structure, mechanical properties and chemical composition (proteins, growth factors). Research on biomaterials, in tissue engineering and regenerative medicine aim to recreate such stimuli with engineered materials in order to induce a specific response of cells at the interface. While traditional biomaterials design has been mostly limited to varying individual cues, increasing interest has arisen on combining several features in recent years in order improve the mimicry of extracellular matrix properties. Tremendous progress in

show abstract

“…Differentiation of mesenchymal stem cells can be directed by ECM stiffness, with softer substrates triggering a more neuronal phenotype and harder substrates inducing progressively muscular or osteoblast-like differentiation [58]. Likewise, differentiation on patterned substrates, namely forcing cells into physical boundaries, can also drive toward a specific phenotype of mesenchymal stem cells [59,60]. Biophysical properties of the ECM are as important for physiological processes as for pathological ones.…”

Section: Modulation Of Ecm / Substrate Stiffness In Toxicological Conmentioning

confidence: 99%

Integrating Biophysics in Toxicology

Favero

Kraegeloh

2020

Cells

View full text Add to dashboard Cite

Integration of biophysical stimulation in test systems is established in diverse branches of biomedical sciences including toxicology. This is largely motivated by the need to create novel experimental setups capable of reproducing more closely in vivo physiological conditions. Indeed, we face the need to increase predictive power and experimental output, albeit reducing the use of animals in toxicity testing. In vivo, mechanical stimulation is essential for cellular homeostasis. In vitro, diverse strategies can be used to model this crucial component. The compliance of the extracellular matrix can be tuned by modifying the stiffness or through the deformation of substrates hosting the cells via static or dynamic strain. Moreover, cells can be cultivated under shear stress deriving from the movement of the extracellular fluids. In turn, introduction of physical cues in the cell culture environment modulates differentiation, functional properties, and metabolic competence, thus influencing cellular capability to cope with toxic insults. This review summarizes the state of the art of integration of biophysical stimuli in model systems for toxicity testing, discusses future challenges, and provides perspectives for the further advancement of in vitro cytotoxicity studies.

show abstract

Substrate topography interacts with substrate stiffness and culture time to regulate mechanical properties and smooth muscle differentiation of mesenchymal stem cells

Cited by 29 publications

References 39 publications

Molecular-Level Interactions between Engineered Materials and Cells

Molecular-Level Interactions between Engineered Materials and Cells

Multifunctional Biomaterials: Combining Material Modification Strategies for Engineering of Cell-Contacting Surfaces

Integrating Biophysics in Toxicology

Contact Info

Product

Resources

About