Substrate Stiffness and Composition Specifically Direct Differentiation of Induced Pluripotent Stem Cells

Macri‐Pellizzeri, Laura; Pelacho, Beatriz; Sancho, Ana; Iglesias-García, Olalla; Simon-Yarza, Ana Maria; Soriano-Navarro, Mario; González-Granero, Susana; García‐Verdugo, José Manuel; De‐Juan‐Pardo, Elena M.; Prósper, Felipe

doi:10.1089/ten.tea.2014.0251

Cited by 71 publications

(60 citation statements)

References 43 publications

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“…For example, adipocytes reside in tissues around 1–10 kPa [88] which overlaps with that of cardiac muscles [90]. This knowledge has been used to create hydrogels seeded with ECM proteins (fibronectin and collagen) and tunable stiffness to direct stem cells towards specific differentiation pathways [91]. Tuned stiffness in conjunction with the appropriate coated proteins on substrates signals cells to the desired phenotypes.…”

Section: Biophysical Regulation Of Cell Reprogrammingmentioning

confidence: 99%

A biomaterial approach to cell reprogramming and differentiation

Long

Kim

et al. 2017

J. Mater. Chem. B

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Cell reprogramming of somatic cells into pluripotent states and subsequent differentiation into certain phenotypes has helped progress regenerative medicine research and other medical applications. Recent research has used viral vectors to induce this reprogramming; however, limitations include low efficiency and safety concerns. In this review, we discuss how biomaterial methods offer potential avenues for either increasing viability and downstream applicability of viral methods, or providing a safer alternative. The use of non-viral delivery systems, such as electroporation, micro/nanoparticles, nucleic acids and the modulation of culture substrate topography and stiffness have generated valuable insights regarding cell reprogramming.

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Section: Biophysical Regulation Of Cell Reprogrammingmentioning

confidence: 99%

A biomaterial approach to cell reprogramming and differentiation

Long

Kim

et al. 2017

J. Mater. Chem. B

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“…In order to achieve this, a variety of scaffold properties, including the scaffolding material, its architecture, mechanical properties, surface chemistry, and topography are often modulated . These scaffold properties have previously been shown to affect cell behavior, particularly differentiation . However, at present the exact relationship between the properties of 3D scaffolds, ECM composition and cellular differentiation has not been completely elucidated.…”

Section: Introductionmentioning

confidence: 99%

Cell‐secreted extracellular matrix formation and differentiation of adipose‐derived stem cells in 3D alginate scaffolds with tunable properties

Guneta

Loh

Choong

2016

J Biomedical Materials Res

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Three dimensional (3D) alginate scaffolds with tunable mechanical and structural properties are explored for investigating the effect of the scaffold properties on stem cell behavior and extracellular matrix (ECM) formation. Varying concentrations of crosslinker (20 - 60%) are used to tune the stiffness, porosity, and the pore sizes of the scaffolds post-fabrication. Enhanced cell proliferation and adipogenesis occur in scaffolds with 3.52 ± 0.59 kPa stiffness, 87.54 ± 18.33% porosity and 68.33 ± 0.88 μm pore size. On the other hand, cells in scaffolds with stiffness greater than 11.61 ± 1.74 kPa, porosity less than 71.98 ± 6.25%, and pore size less than 64.15 ± 4.34 μm preferentially undergo osteogenesis. When cultured in differentiation media, adipose-derived stem cells (ASCs) undergoing terminal adipogenesis in 20% firming buffer (FB) scaffolds and osteogenesis in 40% and 60% FB scaffolds show the highest secretion of collagen as compared to other groups of scaffolds. Overall, this study demonstrates the three-way relationship between 3D scaffolds, ECM composition, and stem cell differentiation.

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“…Source of stem cells may cause the difference in optimal Young's modulus for cardiac differentiation. The optimal Young's modulus for cardiosphere‐derived cells, mouse induced pluripotent stem cells, and embryonic stem cells cardiac differentiate were found to be 31 kPa, 0.6 kPa, and <1 kPa, respectively . In our study, Young's moduli of 25/50 mM and 50/50 mM cross‐linked collagen scaffolds were 15.4 and 31.8 kPa.…”

Section: Discussionmentioning

confidence: 50%

Stiffness‐controlled three‐dimensional collagen scaffolds for differentiation of human Wharton's jelly mesenchymal stem cells into cardiac progenitor cells

Lin¹,

Chen

Lo³

et al. 2016

J Biomedical Materials Res

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Stem cell-based regenerative therapy has emerged as a promising treatment for myocardial infarction. The aim of this study is to develop stiffness-controlled collagen scaffolds to allow proliferation and differentiation of mesenchymal stem cell (MSCs) into cardiac progenitor cells. In this study transforming growth factor β2 (TGF-β2), was used to induce stem cell differentiation into cardiac lineage cells. Collagen scaffolds were cross-linked with cross-linkers, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-Hydroxysuccinimide (NHS). The results showed that collagen scaffolds cross-linked with 25/50 and 50/50 of EDC mM/NHS mM cross-linkers exhibited little difference in shape and size, the scaffold cross-linked with 50/50 of cross-linkers demonstrated better interconnectivity and higher Young's modulus (31.8 kPa) than the other (15.4 kPa). SEM observation showed that MSCs could grow inside the scaffolds and interact with collagen scaffolds. Furthermore, greater viability and cardiac lineage differentiation were achieved in MSCs cultured on stiffer scaffolds. The results suggest that three-dimensional type I collagen scaffolds with suitable cross-linking to adjust for stiffness can affect MSC fate and direct the differentiation of MSCs into cardiac progenitor cells with/without TGF-β2. These stiffness-controlled collagen scaffolds hold great potential as carriers for delivering MSCs differentiated cardiac progenitor cells into infracted hearts. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2234-2242, 2016.

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Substrate Stiffness and Composition Specifically Direct Differentiation of Induced Pluripotent Stem Cells

Cited by 71 publications

References 43 publications

A biomaterial approach to cell reprogramming and differentiation

A biomaterial approach to cell reprogramming and differentiation

Cell‐secreted extracellular matrix formation and differentiation of adipose‐derived stem cells in 3D alginate scaffolds with tunable properties

Stiffness‐controlled three‐dimensional collagen scaffolds for differentiation of human Wharton's jelly mesenchymal stem cells into cardiac progenitor cells

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