Substrate specificity of HMRZ-86 for β-lactamases, including extended-spectrum β-lactamases (ESBLs)

Hanaki, Hideaki; Koide, Yuuji; Yamazaki, Hiroaki; Kubo, Ryoichi; Nakano, Tomota; Atsuda, Koichiro; Sunakawa, Keisuke

doi:10.1007/s10156-007-0563-2

Cited by 13 publications

(11 citation statements)

References 15 publications

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“…Positive BLT results were found for K. oxytoca isolates with high-level expression of their natural chromosomally encoded beta-lactamase, as expected and as previously reported by Livermore et al (17), and one P. mirabilis-producing CTX-M-15 enzyme was positive using BLT as expected (16,17). In fact, these results are in agreement with those of some authors who discourage the use of beta-lactamase substrate drugs as therapy (4).…”

Section: Discussionsupporting

confidence: 90%

“…and Serratia spp. through AmpC stable overproduction (because of lower affinity and activity of hyperproduced AmpC to hydrolyze HMRZ-86 [16]), the NPV was 89% for these species. The overall NPV of 98% in the present study will decrease if the proportion of this latter group among Enterobacteriaceae is higher.…”

Section: Discussionmentioning

confidence: 99%

“…This substrate, initially yellow, turns to a red color when hydrolyzed. The betalactamases that are very efficient at hydrolyzing HMRZ-86 are ESBL (CTX-M) and carbapenemases (IMP), whereas overproduced or acquired AmpC has lower activity (16). In contrast, HMRZ-86 is not hydrolyzed by broad-spectrum beta-lactamases (e.g., SHV-1 and TEM-1) or by basal production of AmpC (17).…”

Section: Strainsmentioning

confidence: 99%

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Evaluation of the βLacta Test, a Rapid Test Detecting Resistance to Third-Generation Cephalosporins in Clinical Strains of Enterobacteriaceae

et al. 2013

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h For decades, third-generation cephalosporins (3GC) have been major drugs used to treat infections due to Enterobacteriaceae; growing resistance to these antibiotics makes the rapid detection of such resistance important. The ␤Lacta test is a chromogenic test developed for detecting 3GC-resistant isolates from cultures on solid media within 15 min. A multicenter prospective study conducted in 5 French and Belgian hospitals evaluated the performance of this test on clinical isolates. Based on antibiotic susceptibility testing, strains resistant or intermediate to cefotaxime or ceftazidime were classified as 3GC resistant, and molecular characterization of this resistance was performed. The rates of 3GC resistance were 13.9% (332/2,387) globally, 9.4% in Escherichia coli (132/1,403), 25.6% in Klebsiella pneumoniae (84/328), 30.3% in species naturally producing inducible AmpC betalactamases (109/360), and 5.6% in Klebsiella oxytoca and Citrobacter koseri (7/124). The sensitivities and specificities of the ␤Lacta test were, respectively, 87.7% and 99.6% overall, 96% and 100% for E. coli and K. pneumoniae, and 67.4% and 99.6% for species naturally producing inducible AmpC beta-lactamase. False-negative results were mainly related to 3GC-resistant strains producing AmpC beta-lactamase. Interestingly, the test was positive for all 3GC-resistant extended-spectrum beta-lactamaseproducing isolates (n ‫؍‬ 241). The positive predictive value was 97% and remained at >96% for prevalences of 3GC resistance ranging between 10 and 30%. The negative predictive values were 99% for E. coli and K. pneumoniae and 89% for the species producing inducible AmpC beta-lactamase. In conclusion, the ␤Lacta test was found to be easy to use and efficient for the prediction of resistance to third-generation cephalosporins, particularly in extended-spectrum beta-lactamase-producing strains.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Strainsmentioning

confidence: 99%

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Evaluation of the βLacta Test, a Rapid Test Detecting Resistance to Third-Generation Cephalosporins in Clinical Strains of Enterobacteriaceae

et al. 2013

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“…The ␤Lacta test (Bio-Rad, Marnes la Coquette, France) is a new chromogenic method based on the use of a yellow substrate (HMRZ-86) that turns to red when hydrolyzed by ESBLs, AmpC ␤-lactamases, and most carbapenemases (9)(10)(11). According to the manufacturer, reading of the results can be performed visually in less than 15 min.…”

mentioning

confidence: 99%

Rapid Detection of β-Lactamase-Hydrolyzing Extended-Spectrum Cephalosporins in Enterobacteriaceae by Use of the New Chromogenic βLacta Test

et al. 2014

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The chromogenic ␤Lacta test developed for the rapid detection of ␤-lactamase-hydrolyzing extended-spectrum cephalosporins in Enterobacteriaceae revealed good performance with extended-spectrum ␤-lactamase (ESBL) producers (97.5% true-positive results). However, false-negative results occurred with chromosomal AmpC hyperproducers and plasmid AmpC producers, whereas uninterpretable results were mostly due to VIM-1 carbapenemase producers and possibly low levels of expressed ESBLs. Detection of Enterobacteriaceae resistant to broad-spectrum cephalosporins, mostly due to production of extended-spectrum ␤-lactamases (ESBL), plasmid AmpC ␤-lactamases, and/or carbapenemases, has become a challenge in clinical microbiology laboratories because of important clinical consequences for infection control purposes and guidance of antimicrobial therapy (1-3). Methods routinely used to detect these organisms are primarily based on susceptibility testing results, either MICs or disk diffusion inhibition zones, as well as on ancillary testing using disk synergy tests with different ␤-lactamase inhibitors or MIC-gradient strips combining ␤-lactams and ␤-lactamase inhibitors (4). Molecular methods based on PCR or microarray hybridization techniques have been also developed (5, 6). In addition, mass spectrometry-based protocols using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and in-house colorimetric tests have been developed to detect the production of ESBLs in less than 4 h (7,8). Increased interest in rapid colorimetric assays has been observed, because of their easy implementation in the routine workflow of clinical laboratories (8).The ␤Lacta test (Bio-Rad, Marnes la Coquette, France) is a new chromogenic method based on the use of a yellow substrate (HMRZ-86) that turns to red when hydrolyzed by ESBLs, AmpC ␤-lactamases, and most carbapenemases (9-11). According to the manufacturer, reading of the results can be performed visually in less than 15 min. In the present study, we assessed the performance of the ␤Lacta test for rapid detection of ␤-lactamasehydrolyzing extended-spectrum cephalosporins in two groups of Enterobacteriaceae clinical isolates. The first group (Table 1) consisted of 338 contemporary clinical isolates collected prospectively (in January to March 2012), and the second group (Table 2) included 106 clinical isolates with ␤-lactamase-mediated resistance mechanisms that were characterized at the molecular level and affected broad-spectrum cephalosporins (12-15). All isolates were recovered at the Ramón y Cajal University Hospital and were identified using both a MicroScan system (Siemens, West Sacramento, CA) and MALDI-TOF MS (Bruker Daltonics, Germany). In addition, the Escherichia coli ATCC 35218 strain (TEM-1 producer) was used as a negative control, whereas the Klebsiella pneumoniae ATCC 700603 strain (SHV-18 producer) was used as a positive control. Susceptibility testing of ␤-lactam antibiotics, including broad-spectrum cephalosporins (cefotaxime, ceftazi...

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“…Metallo-b-lactamases of IMP-type) and to some extent by hyper production of AmpC-lactamases (e.g. FOX-3, ACC-1) (7,8). This study investigated the presence of b LACTA Ò test-positive Enterobacteriales in urinary samples collected in a rural Tanzanian setting at Nkinga Referral Hospital (NRH), Tabora region.…”

mentioning

confidence: 99%

Detection of extended‐spectrum beta‐lactamase production in Enterobacteriales from patients with suspected urinary tract infections, Tabora region, Rural Tanzania

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Malmvall

Masonda³

et al. 2018

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Substrate specificity of HMRZ-86 for β-lactamases, including extended-spectrum β-lactamases (ESBLs)

Cited by 13 publications

References 15 publications

Evaluation of the βLacta Test, a Rapid Test Detecting Resistance to Third-Generation Cephalosporins in Clinical Strains of Enterobacteriaceae

Evaluation of the βLacta Test, a Rapid Test Detecting Resistance to Third-Generation Cephalosporins in Clinical Strains of Enterobacteriaceae

Rapid Detection of β-Lactamase-Hydrolyzing Extended-Spectrum Cephalosporins in Enterobacteriaceae by Use of the New Chromogenic βLacta Test

Detection of extended‐spectrum beta‐lactamase production in Enterobacteriales from patients with suspected urinary tract infections, Tabora region, Rural Tanzania

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