Substrate profiling of the metalloproteinase ovastacin uncovers specific enzyme–substrate interactions and discloses fertilization‐relevant substrates
Matthias Felten,
Ute Distler,
Nele von Wiegen
et al.
Abstract:The metalloproteinase ovastacin is released by the mammalian egg upon fertilization and cleaves a distinct peptide bond in zona pellucida protein 2 (ZP2), a component of the enveloping extracellular matrix. This limited proteolysis causes zona pellucida hardening, abolishes sperm binding, and thereby regulates fertility. Accordingly, this process is tightly controlled by the plasma protein fetuin‐B, an endogenous competitive inhibitor. At present, little is known about how the cleavage characteristics of ovast… Show more
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