Abstract:Protection of the Ca2؉ ATPase (SERCA) from proteinase K digestion has been observed following the addition of Ca 2؉ , Mg 2؉ , and nucleotide and interpreted as a substrate-dependent conformational change (1). The protected digestion site is located on the loop connecting the A domain and the M3 transmembrane helix. We studied by mutational analysis the protective effect of AMP-PCP, an ATP analog that is not utilized for enzyme phosphorylation. We found that the nucleotide protective effect is interfered with b… Show more
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