Substrate-histochemical investigations and ultrahistochemical demonstrations of acid phosphatase in larval and prepupal salivary glands of Drosophila melanogaster

B, von Gaudecker; Em, Schmale

doi:10.1007/bf00220285

Cited by 41 publications

(28 citation statements)

References 21 publications

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“…46,47 Autophagy is characterized by the formation of multiple acidic autophagic vacuoles within the doomed cells followed by massive cellular degeneration. Studies in vertebrate cells have shown that Rel/NF-kB and AP-1 family members play a critical role in controlling apoptosis.…”

Section: Cell Death and Differentiationmentioning

confidence: 99%

AP-1, but not NF-κB, is required for efficient steroid-triggered cell death in Drosophila

Lehmann

Jiang

et al. 2002

Cell Death Differ

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Extensive studies in vertebrate cells have assigned a central role to Rel/NF-kB and AP-1 family members in the control of apoptosis. We ask here whether parallel pathways might function in Drosophila by determining if Rel/NF-kB or AP-1 family members contribute to the steroid-triggered death of larval salivary glands during Drosophila metamorphosis. We show that two of the three Drosophila Rel/NF-kB genes are expressed in doomed salivary glands and that one family member, Dif, is induced in a stage-specific manner immediately before the onset of programmed cell death. Similarly, Djun is expressed for many hours before salivary gland cell death while Dfos is induced in a stage-specific manner, immediately before this tissue is destroyed. We show that null mutations in the three Drosophila Rel/NF-kB family members, either alone or in combination, have no apparent effect on this death response. In contrast, Dfos is required for the proper timing of larval salivary gland cell death as well as the proper induction of key death genes. This study demonstrates a role for AP-1 in the stage-specific steroidtriggered programmed cell death of larval tissues during Drosophila metamorphosis.

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Section: Cell Death and Differentiationmentioning

confidence: 99%

AP-1, but not NF-κB, is required for efficient steroid-triggered cell death in Drosophila

Lehmann

Jiang

et al. 2002

Cell Death Differ

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“…In addition, the level of CRQ expression is sensitive to the amount of apoptosis, as embryos that are cell death defective exhibit a very low level of CRQ expression. Interestingly, crq is transcribed immediately prior to the steroid-activated programmed cell death of larval salivary glands, which exhibit morphology of autophagic cell death [29], [87]. This observation, combined with the display of apoptotic hallmarks, the requirement of caspase activities and transcription of the apoptotic genes rpr, hid and ark, support the hypothesis that apoptotic and autophagic cell death may utilize some common components, including execution and degradation of dying cells.…”

Section: Removal Of Apoptotic Cellsmentioning

confidence: 78%

Genetic regulation of programmed cell death in Drosophila

Lee

Baehrecke

2000

Cell Res

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Programmed cell death plays an important role in maintaining homeostasis during animal development, and has been conserved in animals as different as nematodes and humans. Recent studies of Drosophila have provided valuable information toward our understanding of genetic regulation of death. Different signals trigger the novel death regulators rpr, hid, and grim, that utilize the evolutionarily conserved iap and ark genes to modulate caspase function. Subsequent removal of dying cells also appears to be accomplished by conserved mechanisms. The similarity between Drosophila and human in cell death signaling pathways illustrate the promise of fruit flies as a model system to elucidate the mechanisms underlying regulation of programmed cell death.

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“…After puparium formation only a few secretion granules remain in the cytoplasm of the salivary gland cells (12).…”

Section: Resultsmentioning

confidence: 99%

“…After puparium formation only a few secretion granules remain in the cytoplasm of the salivary gland cells (12). The Oregon stock apparently has a mixture of autosomes, some carrying the gene for protein la, and some carrying the gene for lb.…”

Section: Resultsmentioning

confidence: 99%

“…Ultrastructural (10,11) and electrophoretic investigations have shown that saliva begins to be formed about the middle of the third larval instar. The extrusion of the secretion into the gland lumen begins about three hours before puparium formation.After puparium formation only a few secretion granules remain in the cytoplasm of the salivary gland cells (12).The secretion of the wild-type stock Berlin contains at least five protein components which account for most of the total gland protein (Fig. 1).…”

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confidence: 99%

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Chromosome puff activity and protein synthesis in larval salivary glands of Drosophila melanogaster.

Korge¹

1975

Proc. Natl. Acad. Sci. U.S.A.

130

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Secretion proteins from larval salivar glands of Drosophila melanogaster were analyzed with acrylamide gel electrophoresis. Four fractions were found; three showed electrophoretic variants in different wild-type stocks. Crossbreeding and cytogenetic techniques were used to localize the genes responsible for the two main fractions: The gene for fraction 3 was found to lie within a segment of the third chromosome which includes section 68C; the gene fo'r fraction 4, Sgs-4, was found to lie within section 3C8-3D1 of the X chromosome (1 -3-5). The puffs within these sections of the giant chromosomes are active before and during secretion synthesis and become inactive as secretion synthesis ceases. Larvae of one wild-type stock which lack protein fraction 4 do not exhibit any puffing in 3C. The relative amount of protein 4 in the salivary secretion shows a marked dependence on the dosage of the Sgs-4 gene in both duplication and deficiency genotypes. The active site within puff 3C11-12 apparently contains the structural gene for protein 4.A direct correlation between chromosomal sites which are transcriptionally active and activities in the cytoplasm has been established for puffs of the Balbiani ring type in the giant chromosomes of the salivary glands of Chironomus (1-3) and Acricotopus (4). In Drosophila melanogaster until now only indirect evidence suggesting the existence of such a relationship has been available (5-7). In the work on Drosophila melanogaster to be reported here we have now been able to obtain direct evidence of a cytogenetic nature indicating the location of the genes responsible for certain secretion proteins in individual salivary gland chromosome puffs. Furthermore, the effect of changes in dosage of one of the genes involved has been investigated. (5) y w spl cv f, X chromosome markers which were used for recombination analysis. The salivary gland chromosome locus of w is at 3C2; that of spl, at 3C7; and that of cv, between 4F1-2 -5D1-2. (6) Df(1)N8/dl-49, y Hw m2g4. In the X chromosome carrying Notch-S the section from 3C1 through 3D6-E1 is lacking. The chromosome is kept balanced with the inversion chromosome In(l )delta-49, marked with y HW m2g4. Df(1)scJ4R. All females carry attached X chromosomes and in addition they can carry the free duplication Dp(l;f)z9 of the X chromosome, i.e., section lAl through 3E7-F1;19-20. The males of this stock have an X chromosome with the deficiency Df(1 )SCJ4R, i.e., the section lB to 3A3-C2. The deficiency is compensated by the duplication Dp(lf)z9. (10) Cy/Pm;CxD/Sb. Both second chromosomes carry inversions; one is marked with Cy and one with Pm. One third chromosome carries an inversion and is marked with CxD; the other third chromosome has the normal structure and is marked with Sb.For more information on the genetic markers and their respective salivary chromosome sections see ref. 8. Linkage data for some X chromosome markers are given in Fig. 4.Preparation of the Salivary Glands and Their Chromosomes. The glands of late third instar larva...

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Substrate-histochemical investigations and ultrahistochemical demonstrations of acid phosphatase in larval and prepupal salivary glands of Drosophila melanogaster

Cited by 41 publications

References 21 publications

AP-1, but not NF-κB, is required for efficient steroid-triggered cell death in Drosophila

AP-1, but not NF-κB, is required for efficient steroid-triggered cell death in Drosophila

Genetic regulation of programmed cell death in Drosophila

Chromosome puff activity and protein synthesis in larval salivary glands of Drosophila melanogaster.

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