Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2)

Okumura, A.; Goto, Masaaki; Goto, Tetsuya; Yoshinari, Masao; Masuko, Sadahiko; Katsuki, Tsutomu; Takano, Teruo

doi:10.1016/s0142-9612(00)00415-4

Cited by 130 publications

(116 citation statements)

References 19 publications

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“…The initial attachment of fibroblasts, assuming soft tissue adhesion, on zirconia or Ti discs has also been investigated 13,14) , and these studies have confirmed the similar biological responses of each type of cell on each material. In determining the potential success of epithelium-implant attachment, it is necessary to characterize the response of epithelial cells to zirconia, and initial cell attachment is of particular importance for subsequent cell proliferation, migration and differentiation 15,16) . 19) .…”

Section: Introductionmentioning

confidence: 99%

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

et al. 2012

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Initial attachment of human oral keratinocytes (HOKs) cultured on mirror-surfaced commercially pure titanium (Ti) or yttriastabilized tetragonal zirconia polycrystals (TZP) was investigated. Numbers of viable attached HOKs, their mRNAs and proteins expression of laminin γ2 and integrin β4 were evaluated using the WST-1 assay, quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Localization of laminin γ2 and integrin β4 was observed using immunofluorescent staining. Cell spreading was evaluated by measuring the perimeter of actin on fluorescent stained images, and cell morphology was examined using scanning electron microscopy. At 1 h TZP elicited less of initial attachment than Ti in terms of mRNAs expression and proteins expression of laminin γ2 and integrin β4 (p<0.05). However, at 48 h TZP was showed similar initial attachment in comparison to Ti. Therefore, it was suggested that TZP has a potential to form epithelial attachment like Ti.

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Section: Introductionmentioning

confidence: 99%

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

et al. 2012

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“…The control of osteoblast behavior contributes to improved osteoconductivity. Generally, adhesion of the osteoblasts to inorganic biomaterials plays a predominant role in the regulation of the subsequent differentiation and formation of the extracellular matrix following spreading and motility 1 . Adhesion of the osteoblasts is primarily the result of two subjects, namely the surface characteristics of the biomaterials involved and stimulation from outside the cells.…”

Section: Introductionmentioning

confidence: 99%

Improved Wettability Increases Osteoblastic Adhesion on Hydroxyapatite

Nakamura

Nagai

Yamashita

2011

Phosphorus Research Bulletin

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Osteoblasts are susceptible to the surface characteristics of bioceramics and stimulation from outside the cells. The purpose of this study was to evaluate the effects of electrical polarization on surface characteristics and osteoblastic adhesion. The surface characteristics revealed that electrical polarization had no effect on surface roughness, crystallinity, and constituent elements. According to contact angle measurement, electrically polarized HA, which provides two kinds of surfaces, negatively charged HA (N-HA) and positively charged HA (P-HA), was even more hydrophilic than that of normal HA (HA). Morphological observations and quantitative analyses revealed that the typical adhered cells had a round shape on the HA but had a spindle or fan-like spreading configuration on the N-HA and the P-HA 1 h after seeding. After 3 h of cultivation, the rate of the number of spread cells and the size of focal adhesions on the HA increased and approached that of the N-HA and P-HA. However, the cell areas positively stained for actin, which indicates the degree of cell spreading, were distinctly larger on the N-HA and P-HA than that on the HA. The number of focal adhesions per cell was also less than that on the N-HA and P-HA.

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“…Osteoblasts have been shown to have an important role in the integration of prostheses; migration, attachment, and proliferation of these cells are the first steps leading to bone formation at the bone/implant interface. [1][2][3][4] In this context, several works have focused on biomaterial properties and biocompatibility analysis by investigating the influence of surface composition and topography on cell adhesion and spreading. 3,5,6 Surface-modified substrates were elaborated by adding extracellular matrix (ECM) proteins such as collagen, fibronectin, or RGD peptide sequence coatings to promote osteoblast attachment and adhesion.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4] In this context, several works have focused on biomaterial properties and biocompatibility analysis by investigating the influence of surface composition and topography on cell adhesion and spreading. 3,5,6 Surface-modified substrates were elaborated by adding extracellular matrix (ECM) proteins such as collagen, fibronectin, or RGD peptide sequence coatings to promote osteoblast attachment and adhesion. [7][8][9] However, little is known about ECM remodeling displayed by osteoblasts, the main cells responsible for bone remodeling in concert with osteoclasts, in response to the biomaterials.…”

Section: Introductionmentioning

confidence: 99%

“…19,20 MMPs are implicated in physiological processes (embryogenesis, bone development) and in pathological situations (malignant tumors, rheumatoid arthritis, and periodontal diseases). 12,[21][22][23] Their proteolytic activity is regulated endogenously by tissue inhibitors (TIMPs), cytokines, or by bone resorbing agents such as parathyroid hormone or 1,25(OH) 2 vitamin D 3 . 17,24 -26 In pathological osteolysis observed in aseptic loosening of total hip replacement, the expression and synthesis of MMPs and TIMPs have been shown to be stimulated in osteoblasts directly by wear particle ingestion.…”

Section: Introductionmentioning

confidence: 99%

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Matrix metalloproteinases MMP‐2, ‐9 and tissue inhibitors TIMP‐1, ‐2 expression and secretion by primary human osteoblast cells in response to titanium, zirconia, and alumina ceramics

Oum'Hamed

Garnotel

Josset

et al. 2003

J Biomedical Materials Res

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Osteogenic properties of bone cells are a key parameter governing osseointegration of implant devices. In this context, osteoblasts have a central role via extracellular matrix synthesis and remodeling that they regulate through different protease activity. In this study, we have analyzed the expression of two matrix metalloproteinases (MMPs): MMP-2 (72 kDa) and MMP-9 (92 kDa) and their specific tissue inhibitors TIMP-1 and TIMP-2 in primary human osteoblastic cells. The effect of titanium, zirconia, and alumina ceramics on the synthesis of these proteases was assessed using reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and zymographic analysis. Our results showed that osteoblasts express MMP-2 and -9 mRNA. Furthermore, MMP-2 mRNA expression was decreased by titanium and increased by alumina whereas zirconia did not have any significant effect. Conversely, MMP-9 mRNA expression was stimulated by titanium but decreased with zirconia, whereas alumina induced no significant changes. Zymographic analysis has evidenced pro-MMP-2 gelatinolytic activity in all cell populations with time-dependent increase profile; pro-MMP-9, however, was not detected. Enzyme-linked immunosorbent assay data confirmed the production of MMP-2 and very low levels of MMP-9. In addition, TIMP-1 was secreted in 24-hcultured cells and increased to maximal level at 48 -72 h whereas TIMP-2 levels were very low. The interactions between human osteoblasts and the studied biomaterials altered both MMP-2, -9 and TIMP-1expression indicating that biomaterials may influence osseointegration and bone remodeling.

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Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2)

Cited by 130 publications

References 19 publications

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

Initial attachment of human oral keratinocytes cultured on zirconia or titanium

Improved Wettability Increases Osteoblastic Adhesion on Hydroxyapatite

Matrix metalloproteinases MMP‐2, ‐9 and tissue inhibitors TIMP‐1, ‐2 expression and secretion by primary human osteoblast cells in response to titanium, zirconia, and alumina ceramics

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