Substrate-adsorbed collagen and cell secreted fibronectin concertedly induce cell migration on poly(lactide–glycolide) substrates

Tjia, Jane S.

doi:10.1016/s0142-9612(99)00153-2

Cited by 53 publications

(42 citation statements)

References 40 publications

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“…This type of migration called haptotaxis may be augmented by the other two, namely chemokinesis, the random cellular migration in response to soluble factors, and chemotaxis, the migration towards a positive gradient of soluble attractant (Zetter and Brightman, 1990). The addition of soluble fibronectin, for instance, to cells moving on immobilised collagen leads to significantly enhanced migration (Tjia et al, 1999). Similarly, soluble thrombospondin-1, acting via ␤ 3 integrin ligation, increases EC migration on a nonsoluble substrate (Taraboletti et al, 1990).…”

Section: Facilitated Migration: a Key To Spontaneous Endothelializationmentioning

confidence: 99%

Engineering of vascular ingrowth matrices: Are protein domains an alternative to peptides?

2001

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Anastomotic intimal hyperplasia and surface thrombogenicity are the main reasons for the high failure rate of prosthetic small-diameter vascular grafts. While anastomotic intimal hyperplasia is a multifactorial event, ongoing surface thrombogenicity is primarily caused by the lack of an endothelium, even after years of clinical implantation. After decades of poorly performing synthetic artery-grafts, tissue engineering has emerged as a promising approach to generate biologically functional bio-synthetic hybrid grafts mimicking native arteries regarding the presence of an endothelial lining on the blood surface. "In vitro endothelialization" represented the first generation of such tissue-engineered vascular grafts, utilising cell culture techniques for the creation of a confluent autologous endothelium on ePTFE grafts. The clinical long-term results with this method in almost 200 patients are highly encouraging, showing patencies equal to vein grafts. Since "in vitro endothelialization" requires cell culture facilities, it will always be confined to large centres. Therefore, research of the 1990s turned to the development of spontaneously endothelializing implants, to make tissue-engineered grafts amenable to the entire vascular-surgical community. Apart from scaffold designs allowing transmural ingrowth, biological signalling through a facilitating ingrowth matrix holds a key to spontaneous endothelialization. In biological signalling, the increasingly deeper understanding of bio-active molecules and the discovery of domains and peptide sequences during the 1980s created the expectation in the 1990s that peptide signalling may be all that is needed. This present review highlights the possible problems associated with such a reductionist approach. Using the fibronectin molecule, we demonstrated that domains may be more suitable modules in tissue engineering than peptide sequences.

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Section: Facilitated Migration: a Key To Spontaneous Endothelializationmentioning

confidence: 99%

Engineering of vascular ingrowth matrices: Are protein domains an alternative to peptides?

2001

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“…In addition to attachment studies, collagen type I, laminin, and fibronectin have been found to affect migration, proliferation, and differentiation of keratinocytes. Adsorption of collagen type I on both poly(lactide-glycolide) and polystyrene substrates enhanced keratinocyte migration, 16,17 adsorption of laminin on polystyrene stimulated keratinocyte migration and proliferation, 16,18 and adsorption of fibronectin on polystyrene promoted keratinocyte migration and inhibited terminal differentiation. 17,19 Together, these studies demonstrate that biochemically modified substrates can be used to examine the mechanisms by which extracellular matrix proteins direct keratinocyte function.…”

Section: Introductionmentioning

confidence: 99%

Conjugation of extracellular matrix proteins to basal lamina analogs enhances keratinocyte attachment

Bush

Downing

Walsh

et al. 2006

J Biomedical Materials Res

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The dermal-epidermal junction of skin contains extracellular matrix proteins that are involved in initiating and controlling keratinocyte signaling events such as attachment, proliferation, and terminal differentiation. To characterize the relationship between extracellular matrix proteins and keratinocyte attachment, a biomimetic design approach was used to precisely tailor the surface of basal lamina analogs with biochemistries that emulate the native biochemical composition found at the dermal-epidermal junction. A high-throughput screening device was developed by our laboratory that allows for the simultaneous investigation of the conjugation of individual extracellular matrix proteins (e.g. collagen type I, collagen type IV, laminin, or fibronectin) as well as their effect on keratinocyte attachment, on the surface of an implantable collagen membrane. Fluorescence microscopy coupled with quantitative digital image analyses indicated that the extracellular matrix proteins adsorbed to the collagen-GAG membranes in a dose-dependent manner. To determine the relationship between extracellular matrix protein signaling cues and keratinocyte attachment, cells were seeded on protein-conjugated collagen-GAG membranes and a tetrazolium-based colorimetric assay was used to quantify viable keratinocyte attachment. Our results indicate that keratinocyte attachment was significantly enhanced on the surfaces of collagen membranes that were conjugated with fibronectin and type IV collagen. These findings define a set of design parameters that will enhance keratinocyte binding efficiency on the surface of collagen membranes and ultimately improve the rate of epithelialization for dermal equivalents.

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“…The advantage of combining a synthetic bioresorbable polymer with an ECM protein coating for EC attachment and functioning was demonstrated by several authors [42][43][44][45]. Improved endothelial cell lining of PET prosthesis modified with ECM proteins was also reported [46][47][48][49].…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, Tjia et al [42] showed that pretreatment of PLG substrate with Co type I together with cell secreted FN concertedly induces the migration of human keratinocytes to an extent much greater than in the case of FN or Co pretreatment only. They conclude that the roles of ECM proteins are not cross-functional, and that Co-FN interactions may present FN in a suitable conformation, thus promoting cell adhesion and migration [42]. This supports the idea that composite ECM protein assemblies may be better substrata for adhesion, proliferation and retention of cells seeded on vascular grafts [48].…”

Section: Discussionmentioning

confidence: 99%

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Filova¹

2014

J Tissue Sci Eng

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Substrate-adsorbed collagen and cell secreted fibronectin concertedly induce cell migration on poly(lactide–glycolide) substrates

Cited by 53 publications

References 40 publications

Engineering of vascular ingrowth matrices: Are protein domains an alternative to peptides?

Engineering of vascular ingrowth matrices: Are protein domains an alternative to peptides?

Conjugation of extracellular matrix proteins to basal lamina analogs enhances keratinocyte attachment

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

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