Substitutions and Deletions of Genes Related to Grain Hardness in Wheat and Their Effect on Grain Texture

Tranquilli, Gabriela; Heaton, James; Chicaiza, Oswaldo; Dubcovsky, Jorge

doi:10.2135/cropsci2002.1812

Cited by 81 publications

(80 citation statements)

References 22 publications

(26 reference statements)

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“…Simultaneous deletion of both Pin-D1 loci in hexaploid wheat results in an average hardness index approximately 5% higher than those observed in genotypes with individual mutations (Tranquilli et al 2002). The absence of Pina-D1 and Pinb-D1 mRNA transcripts in durum wheat (T. turgidum, AABB) is consistent with its lack of the hexaploid wheat (T. aestivum, AABBDD) D genome and results in very hard endosperm texture (Giroux et al 2000).…”

Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 77%

“…Grain softness proteins (GSPs) are closely related to puroindolines (Gautier et al 2000) and the grain softness protein-1 gene (Gsp-D1) is closely linked to the Ha locus in wheat (Jolly et al 1996) and T. monococcum L. (Gsp-Am1). Despite the close relationship among these three genes, there is no clear evidence of the role of Gsp-1 on grain kernel hardness (Tranquilli et al 2002).…”

Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 97%

“…In wheat, the genetic control of kernel hardness is well understood, and is largely controlled by the action of two tightly linked puroindoline a (Pina-D1) and puroindoline b (Pinb-D1) genes at the Hardness (Ha) locus on the distal end of the short arm of chromosome 5D (Gautier et al 2000;Sourdille et al 1996;Giroux et al 2000Giroux et al , 1998Tranquilli et al 2002;Ram et al 2002;Turnbull et al 2003). These genes code for two cystinerich puroindoline a (PINA) and b proteins (PINB; Morris 2002) both of which contain a tryptophan domain that interacts with lipids located on the surface of starch granules (Gautier et al 2000;Bhave and Morris, 2008).…”

Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 99%

“…Based on these studies, at least 25 alleles at the Pinb-D1 locus have been reported (Lillemo and Morris 2000;Morris et al 2001;Tranquilli et al 2002;Ram et al 2005;Xia et al 2005;Chen et al 2006Chen et al , 2007Bhave and Morris 2008), but the Pinb-D1b allele is the most prevalent allele in US, Norwegian, Japanese, South Korean, Indian, and Chinese varieties (Chen et al 2006;Lillemo and Morris 2000;Morris et al 2001;Ikeda et al 2005;Ram et al 2005;Xia et al 2005). Unlike the Pinb-D1 locus, only a few mutations conferring kernel hardness have been reported at Pina-D1.…”

Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 99%

“…BsrB1 recognizes a 5?-CCGCTC-3? restriction site present in Pinb-D1b (Tranquilli et al 2002), whereas the lack of a PvuII restriction site (5?-CAGCTG-3?) is indicative of the Pinb-D1c allele (Lillemo and Morris 2000).…”

Section: Genotyping and Sequencing Of Puroindoline Allelesmentioning

confidence: 99%

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Puroindoline allele diversity in Canadian and northern US hard spring wheat varieties differing in kernel hardness

Matus‐Cádiz¹,

Pozniak²,

Hucl³

2008

Can. J. Plant Sci.

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Matus-Ca´diz, M. A., Pozniak, C. J. and Hucl, P. J. 2008. Puroindoline allele diversity in Canadian and northern US hard spring wheat varieties differing in kernel hardness. Can. J. Plant Sci. 88: 873Á883. Kernel hardness, one of the most important factors in determining the end-use suitability of wheat (Triticum aestivum L.), is largely influenced by puroindoline proteins a (PINA) and b (PINB). Soft texture is wildtype (Pina-D1a/Pinb-D1a) with hard texture being determined by either Pina-D1 gene deletion or point mutations in Pinb-D1a. The objectives of this study were to determine kernel hardness indices (HI) and Pina-D1/Pinb-D1 allelic diversity in a diverse set of 81 Canadian (representing eight wheat classes) and 49 US hard spring wheat varieties. Varieties were grouped into two experimental sets grown in replicated trials in 2004Á2006 at Saskatoon, SK. Variation existed among varieties with HI means ranging from 21.7 (CDC Zorba) to 72.7 (AC Morse) in exp. 1 and from 25.0 (HY320) to 66.1 (Norlander) in exp. 2. Only AC Andrew, CDC Zorba, and Red Fife were soft kernel textured and carried the wildtype Pina-D1a/Pinb-D1a alleles. The majority of Canadian varieties were Pina-D1a/Pinb-D1b (69%), while a greater frequency of US varieties was Pina-D1b/Pinb-D1a (55%). Only four varieties, all from Quebec-based breeding programs, carried the Pinb-D1c allele. On average, varieties with the Pina-D1b allele had significantly harder kernels than those carrying the Pinb-D1b mutation. Durum wheat (T. turgidum L. var durum) varieties, included as hard-kernelled controls, possessed significantly harder kernels (average HI 071.2) than spring wheat varieties with Pina-D1b (65.3). Varieties carrying Pina-D1b (null PINA) were harder than those carrying hardness mutations at the Pinb-D1 locus, but considerable overlap in hardness was evident among genotypic classes. This work represents the first extensive Pin genotyping combined with HI phenotyping survey of Canadian wheat varieties, which is expected to aid breeders in understanding how Pina-D1/Pinb-D1 allele composition is currently associated with kernel hardness across Canadian wheat classes.

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Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 77%

Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 97%

Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 99%

Section: Mots Clé S: Triticum Aestivum Durete´du Grain Puroindolinesmentioning

confidence: 99%

Section: Genotyping and Sequencing Of Puroindoline Allelesmentioning

confidence: 99%

See 3 more Smart Citations

Puroindoline allele diversity in Canadian and northern US hard spring wheat varieties differing in kernel hardness

Matus‐Cádiz¹,

Pozniak²,

Hucl³

2008

Can. J. Plant Sci.

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Wheat Proteins

Juhász

Békés²,

Wrigley

2014

Applied Food Protein Chemistry

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Designations used by companies to distinguish their products are often claimed as trademarks. All brand names and product names used in this book are trade names, service marks, trademarks or registered trademarks of their respective owners. The publisher is not associated with any product or vendor mentioned in this book.Limit of Liability/Disclaimer of Warranty: While the publisher and author(s) have used their best efforts in preparing this book, they make no representations or warranties with respect to the accuracy or completeness of the contents of this book and specifically disclaim any implied warranties of merchantability or fitness for a particular purpose. It is sold on the understanding that the publisher is not engaged in rendering professional services and neither the publisher nor the author shall be liable for damages arising herefrom. If professional advice or other expert assistance is required, the services of a competent professional should be sought.

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Sensory and instrumental texture measurements for assessing grape seed parameters during fruit development

et al. 2013

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This research showed that both seed sensory attributes and instrumental texture properties are indicators of grape ripening. However, these properties could be affected by the harvest season and growing location. As no clear correlation was found between the seed sensory attributes and instrumental texture parameters, a revision of the sensory method available in the literature could be suggested.

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Substitutions and Deletions of Genes Related to Grain Hardness in Wheat and Their Effect on Grain Texture

Cited by 81 publications

References 22 publications

Puroindoline allele diversity in Canadian and northern US hard spring wheat varieties differing in kernel hardness

Puroindoline allele diversity in Canadian and northern US hard spring wheat varieties differing in kernel hardness

Wheat Proteins

Sensory and instrumental texture measurements for assessing grape seed parameters during fruit development

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