Substitutional editing of Heterocapsa triquetra chloroplast transcripts and a folding model for its divergent chloroplast 16S rRNA

Dang, Yunkun; Green, Beverley R.

doi:10.1016/j.gene.2009.04.006

Cited by 39 publications

(51 citation statements)

References 40 publications

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“…2). In each clone sequenced, we observed poly(U) tracts similar to those previously reported in peridinin dinoflagellates and C. velia (29,31,34). Each sequenced clone was polyuridylylated 8-22 nt downstream of the translation termination codon (Fig.…”

Section: ′supporting

confidence: 80%

“…Characterizing the factors involved in polyuridylylation and editing will be a major task, as the effector proteins involved in chloroplast transcript editing and 3′ modification remain poorly characterized even in plants (20,40,41), and poly(U) polymerases involved in nuclear and mitochondrial RNA metabolism of other lineages appear to have arisen independently from a range of nucleotide polymerase families (41,42). In addition, it remains to be shown whether polyuridylylation and editing are functionally interconnected to other features of chloroplast RNA metabolism in K. mikimotoisuch as transcript end maturation-as has been hypothesized in peridinin dinoflagellate lineages (30,33,34).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to polyuridylylation, transcripts in chloroplasts of peridinin dinoflagellates undergo an extensive array of base editing events. Outside dinoflagellates, chloroplast RNA editing has been reported only in land plants (3,32,34). We therefore tested if chloroplast transcripts in K. mikimotoi were also edited, by comparing the sequences of oligo(dA) primed RT-PCR products for each gene with similar sequences obtained from genomic DNA.…”

Section: ′mentioning

confidence: 99%

“…Although the extent of bias varied, the psbA, psbC, psbD, and psaA transcripts appeared to contain particularly high frequencies of uracil-to-cytosine conversions (Fig. 3B), in contrast to peridinin dinoflagellates, which predominantly perform adenine-to-guanine conversions, or plant chloroplasts, which typically perform cytosine-to-uracil conversions (3,20,31,32,34). We could not observe any differences between the RT-PCR product and genomic DNA sequences for E. huxleyi or P. tricornutum psbA and psbD (37,39), suggesting that editing is absent from both haptophyte and diatom chloroplast lineages.…”

Section: ′mentioning

confidence: 99%

“…In peridinin dinoflagellate chloroplasts, some editing events are found only in polyuridylylated transcripts (34). We investigated if there was a relationship between transcript polyuridylylation and editing in K. mikimotoi by directly sequencing RT-PCR products for nonpolyuridylylated transcripts of each gene, using gene-specific cDNA primers that annealed to the 3′ UTR of each gene ∼100 nt downstream of the poly(U) site and the same PCR forward primers as before.…”

Section: ′mentioning

confidence: 99%

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Functional remodeling of RNA processing in replacement chloroplasts by pathways retained from their predecessors

Dorrell

Howe

2012

Proc. Natl. Acad. Sci. U.S.A.

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Chloroplasts originate through the endosymbiotic integration of a host and a photosynthetic symbiont, with processes established within the host for the biogenesis and maintenance of the nascent chloroplast. It is thought that several photosynthetic eukaryotes have replaced their original chloroplasts with others derived from different source organisms in a process termed "serial endosymbiosis of chloroplasts." However, it is not known whether replacement chloroplasts are affected by the biogenesis and maintenance pathways established to support their predecessors. Here, we investigate whether pathways established during a previous chloroplast symbiosis function in the replacement chloroplasts of the dinoflagellate alga Karenia mikimotoi. We show that chloroplast transcripts in K. mikimotoi are subject to 3′ polyuridylylation and extensive sequence editing. We confirm that these processes do not occur in free-living relatives of the replacement chloroplast lineage, but are otherwise found only in the ancestral, red algalderived chloroplasts of dinoflagellates and their closest relatives. This indicates that these unusual RNA-processing pathways have been retained from the original symbiont lineage and made use of by the replacement chloroplast. Our results constitute an addition to current theories of chloroplast evolution in which chloroplast biogenesis may be radically remodeled by pathways remaining from previous symbioses.organelle | fucoxanthin | haptophyte | poly(U) | alveolate

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Section: ′supporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: ′mentioning

confidence: 99%

Section: ′mentioning

confidence: 99%

Section: ′mentioning

confidence: 99%

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Functional remodeling of RNA processing in replacement chloroplasts by pathways retained from their predecessors

Dorrell

Howe

2012

Proc. Natl. Acad. Sci. U.S.A.

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Organellar RNA editing

Chateigner‐Boutin

Small

2011

WIREs RNA

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RNA editing is a term used for a number of mechanistically different processes that alter the nucleotide sequence of RNA molecules to differ from the gene sequence. RNA editing occurs in a wide variety of organisms and is particularly frequent in organelle transcripts of eukaryotes. The discontiguous phylogenetic distribution of mRNA editing, the mechanistic differences observed in different organisms, and the nonhomologous editing machinery described in different taxonomic groups all suggest that RNA editing has appeared independently several times. This raises questions about the selection pressures acting to maintain editing that are yet to be completely resolved. Editing tends to be frequent in organisms with atypical organelle genomes and acts to correct the effect of DNA mutations that would otherwise compromise the synthesis of functional proteins. Additional functions of editing in generating protein diversity or regulating gene expression have been proposed but so far lack widespread experimental evidence, at least in organelles.

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Complex Plastids Across the Eukaryotes: An Overview of Inherited and Convergently Evolved Characters

Novák Vanclová,

Dorrell

2024

Endosymbiotic Organelle Acquisition

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Substitutional editing of Heterocapsa triquetra chloroplast transcripts and a folding model for its divergent chloroplast 16S rRNA

Cited by 39 publications

References 40 publications

Functional remodeling of RNA processing in replacement chloroplasts by pathways retained from their predecessors

Functional remodeling of RNA processing in replacement chloroplasts by pathways retained from their predecessors

Organellar RNA editing

Complex Plastids Across the Eukaryotes: An Overview of Inherited and Convergently Evolved Characters

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