Substitution saturation and nuclear paralogs of commonly employed phylogenetic markers in the Caryophyllidea, an unusual group of non-segmented tapeworms (Platyhelminthes)

Brabec, Jan; Scholz, Tomáš; Králová-Hromadová, Ivica; Bazsalovicsová, Eva; Olson, Peter D.

doi:10.1016/j.ijpara.2012.01.005

Cited by 54 publications

(42 citation statements)

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“…However, the phenomenon of NUMTs, as described by Brabec et al (2012) in Slovak C. laticeps individuals from freshwater bream A. brama and common carp Cyprinus carpio, and Slovak C. brachycollis specimen from freshwater bream A. brama (see Fig. 1), was not noticed in any specimens analysed in the present study.…”

Section: Discussioncontrasting

confidence: 43%

“…-gobioninae) known from a relatively small area in China, were found to be morphologically clearly different species (Xi et al 2009. However, they are almost indistinguishable in their ribosomal internal transcribed spacer 2 (ITS2), ssrDNA, lsrDNA, and mitochondrial cox1 and nicotinamide dehydrogenase subunit III (nad3) sequences , Králová-Hromadová et al 2012. It was assumed that K. saurogobii, after switching to a new, unrelated fish host, may have undergone morphological divergence as a result of ongoing sympatric speciation, but this process has not been accompanied by corresponding nucleotide changes in the standard genes used as informative markers in our analyses .…”

Section: Discussionmentioning

confidence: 99%

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Conflict between morphology and molecular data: a case of the genus Caryophyllaeus (Cestoda: Caryophyllidea), monozoic tapeworms of cyprinid fishes

Bazsalovicsová¹,

Králová-Hromadová²,

Brabec³

et al. 2014

FOLIA PARASIT

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Molecular analysis of an extensive specimen collection of morphologically distinct tapeworms of the genus Caryophyllaeus Gmelin, 1790 (Cestoda: Caryophyllidea), parasites of cyprinid fishes in the Palaearctic Region, brought evidence of hostrelated plasticity in critical morphological characters widely used for species circumscription and classification of these tapeworms. The results obtained here do not support the traditionally used morphological concept of species-defining characters of the order Caryophyllidea Carus, 1863, especially due to high morphological plasticity of the scolex and the anterior end of the body. Several morphotypes within both Caryophyllaeus laticeps (Pallas, 1781) and Caryophyllaeus brachycollis Janiszewska, 1953 generally corresponding to different hosts and geographical regions, were recognised likely suggesting host-and geography-related intraspecific morphological variability. Genetic data confirmed euryxenous host specificity of both species, most profoundly C. laticeps, but did not support the existence of cryptic species. In fact, some of the fish hosts may harbour both of the congeneric species including several of their respective morphotypes. The pattern of morphological and genetic divergence observed in both cestode species studied indicates a scenario of possible host range extensions and subsequent parasite diversification. If molecular sequence variability and host-related morphological polymorphism are confirmed in other groups of monozoic cestodes, it may complicate species identification and straightforward classification of these parasites.

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Section: Discussioncontrasting

confidence: 43%

Section: Discussionmentioning

confidence: 99%

Conflict between morphology and molecular data: a case of the genus Caryophyllaeus (Cestoda: Caryophyllidea), monozoic tapeworms of cyprinid fishes

Bazsalovicsová¹,

Králová-Hromadová²,

Brabec³

et al. 2014

FOLIA PARASIT

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“…The protocol for PCR amplification of the large subunit nuclear ribosomal RNA gene (lsrDNA, D1-D3 domains) and sequencing were done as described in Brabec et al (2012). Contiguous sequences were assembled using Geneious version R8 (http://www.geneious.com/; Kearse et al 2012) and submitted to GenBank.…”

Section: Methodsmentioning

confidence: 99%

A new genus of proteocephalid tapeworm (Cestoda) from the marbled swamp eel Synbranchus marmoratus Bloch (Synbranchiformes: Synbranchidae) in the River Parana basin, Argentina

Arredondo¹,

Alves²,

Pertierra³

2017

FOLIA PARASIT

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Synbranchiella gen. n. is proposed to accommodate Synbranchiella mabelae sp. n. (Proteocephalidae: Monticelliinae) from the intestine of the marbled swamp eel Synbranchus marmoratus Bloch, in the River Colastiné, a tributary of the middle River Paraná in Argentina. The new genus is placed in the Monticelliinae because of the cortical position of the genital organs. It differs from all known monticelliine genera by the following combination of characters: (i) scolex robust, with a conical apex, without metascolex; (ii) biloculate suckers with a conspicuous septum separating unequally-sized loculi and a robust non-adherent area, lacking free posterior margin; (iii) vitelline follicles in two narrow lateral bands, extended throughout the nearly entire proglottid length; (iv) vagina always anterior to the cirrus-sac, with an inconspicuous vaginal sphincter; (v) a genital pore pre-equatorial. Scanning electron microscopy revealed three types of microtriches on the tegument surface: acicular and capiliform filitriches and gladiate spinitriches. A phylogenetic analysis of the large subunit nuclear ribosomal RNA gene (lsrDNA, D1-D3 domains) confirms that S. mabelae represents an independent lineage within a large clade comprised mainly from Neotropical taxa parasitising catfishes. This is the second proteocephalidean cestode described from a Neotropical synbranchiform fish host.

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“…The protocol for DNA isolation, PCR amplification of the nuclear ribosomal RNA genes (ssrD-NA, lsrDNA) and sequencing followed the protocol of Brabec et al (2012). Contiguous sequences were assembled using Geneious version 7 (http://www.geneious.com/, Kearse et al 2012) and single-gene alignments created using the E-INS-i algorithm of the program MAFFT (Katoh and Standley 2013) implemented therein.…”

Section: Molecular Data Generation and Analysesmentioning

confidence: 99%

“…almost three decades, of neglect by North American parasitologists is unfortunate, because caryophyllidean cestodes represent an interesting model for studies of unusual molecular and cytogenetic phenomena (Králová-Hromadová et al 2010, Špakulová et al 2011, Brabec et al 2012, and phenotypic, possibly host-related plasticity of some species (Barčák et al 2014, Bazsalovicsová et al 2014, Hanzelová et al 2015.…”

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confidence: 99%

A synoptic review of Promonobothrium Mackiewicz, 1968 (Cestoda: Caryophyllidea), parasites of suckers (Catostomidae) in North America, with description of two new species

et al. 2016

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Monozoic cestodes of the recently amended genus Promonobothrium Mackiewicz, 1968 (Cestoda: Caryophyllidea), parasites of suckers (Cypriniformes: Catostomidae) in North America, are reviewed, with information on their host specificity, distribution and data on the scolex morphology of seven species studied for the first time using scanning electron microscopy (SEM). Evaluation of type and voucher specimens from museum collections and newly collected material of most species indicated the following valid nominal species: Promonobothrium minytremi Mackiewicz, 1968 (type species); P. ingens ; P. hunteri (Mackiewicz, 1963); P. ulmeri (Calentine et Mackiewicz, 1966); P. fossae (Williams, 1974) and P. mackiewiczi (Williams, 1974). Rogersus Williams, 1980 with its only species R. rogersi is transferred to Promonobothrium based on morphological and molecular data. Promonobothrium currani sp. n. and P. papiliovarium sp. n. are described from Ictiobus bubalus (Rafinesque) and Ictiobus niger (Rafinesque), and Erimyzon oblongus (Mitchill), respectively. The newly described species can be distinguished from the other congeners by the morphology of the scolex, the position of the anteriormost vitelline follicles and testes, the presence of postovarian vitelline follicles and the shape of the ovary. Molecular phylogenetic analyses of six species based on sequences of the small and large subunits of the nuclear ribosomal RNA genes (ssrDNA, lsrDNA) confirmed the monophyletic status of the genus and supported the validity of the species analysed. A key to identification of all species of Promonobothrium based on morphological characteristics is provided.

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Substitution saturation and nuclear paralogs of commonly employed phylogenetic markers in the Caryophyllidea, an unusual group of non-segmented tapeworms (Platyhelminthes)

Cited by 54 publications

References 50 publications

Conflict between morphology and molecular data: a case of the genus Caryophyllaeus (Cestoda: Caryophyllidea), monozoic tapeworms of cyprinid fishes

Conflict between morphology and molecular data: a case of the genus Caryophyllaeus (Cestoda: Caryophyllidea), monozoic tapeworms of cyprinid fishes

A new genus of proteocephalid tapeworm (Cestoda) from the marbled swamp eel Synbranchus marmoratus Bloch (Synbranchiformes: Synbranchidae) in the River Parana basin, Argentina

A synoptic review of Promonobothrium Mackiewicz, 1968 (Cestoda: Caryophyllidea), parasites of suckers (Catostomidae) in North America, with description of two new species

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