Substitution pattern of 3-deoxy-d-manno-oct-2-ulosonic acid in bacterial lipopolysaccharides investigated by methylation analysis of whole LPS

Rybka, Jacek; Zielińska-Kuźniarz, Katarzyna; Korzeniowska‐Kowal, Agnieszka; Sondej, Aneta; Gamian, Andrzej

doi:10.1016/j.carres.2003.07.006

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Kdo Derivatives and Analysis Of Their Mass Spectra1

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2006

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“…Bacterial strains of E. coli K12 C600 (PCM 2184), E. coli strain B (PCM 1935) and Vibrio cholerae NAG (clinical isolate) were obtained from the collection of the Institute of Immunology and Experimental Therapy, Wrocyaw, Poland. Lipopolysaccharides were prepared with phenol-water extraction method (Westphal and Jann, 1965) and purified on Sepharose 2B column in 0.01 M NH 4 HCO 3 (Romanowska, 1967) as detailed previously (Rybka et al, 2003). Samples of rat serum from previous investigation (Szponar et al, 2003) were used.…”

Section: Methodsmentioning

confidence: 99%