Substitution of methyl tert.-butyl ether for diethyl ether in the standardized thin-layer chromatographic method for lichen products

Culberson, Chicita F.; Johnson, Anita

doi:10.1016/s0021-9673(00)81336-9

Cited by 290 publications

(180 citation statements)

References 7 publications

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“…Nomenclature of non-soluble pigments follows Meyer and Printzen (2000). Thin layer chromatography was carried out according to Culberson (1972) and Culberson and Johnson (1982), and high performance liquid chromatography according to Elix et al (2003).…”

Section: Morphology and Anatomymentioning

confidence: 99%

A molecular phylogeny of the Lecanora varia group, including a new species from western North America

et al. 2010

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Section: Morphology and Anatomymentioning

confidence: 99%

A molecular phylogeny of the Lecanora varia group, including a new species from western North America

et al. 2010

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“…All material has been studied with thin-layer chromatography (Culberson & Ammann 1979) with modified solvent B (Culberson & Johnson 1982). Anatomical measurements of the cortex (C), medulla (M) and central axis (A) were established according to the method given in Clerc (1984a).…”

Section: Methodsmentioning

confidence: 99%

Notes on the Genus Usnea Dill. Ex Adanson

Clerc

1997

The Lichenologist

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“…10) onto Merck silica gel 60 F254 glass plates. Chemical analyses were performed in three routinely used solvent systems: A (toluene/ dioxane/ acetic acid: 180:45:5), B (n-hexane/MTBE/ formic acid-65:40:10) and C (toluene/ acetic acid-200:30) [16,17]. After brief drying, the plates are visualized on UV light (254 nm and 365 nm) and are sprayed with a stable solution of anisaldehyde sulfuric reagent (ANS: anisaldehyde/acetic acid/methanol/sulfuric acid-0.5:10:85:5) until wet, and then heated at 110ºC until development of spots, which were visualized on visible light [18].…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

The Use of Anisaldehyde Sulfuric Acid as an Alternative Spray Reagent in TLC Analysis Reveals Three Classes of Compounds in the Genus <em>Usnea</em> Adans. (Parmeliaceae, lichenized Ascomycota)

Gerlach¹,

Gadea²,

Silveira³

et al. 2018

Preprint

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Presence and identity of secondary metabolites are two of the main components of lichen taxonomy. Aromatic compounds formed via the acetyl-polymalonate pathway are the most studied lichen substances. In addition, compounds derived from the mevalonic acid pathway (e.g. terpenes and steroids) are sometimes detected in the medulla. However, their identity and value as diagnostic characters in the genus Usnea are yet poorly understood despite the fact that they were mentioned in several taxonomical papers. We conclude that i) aside from the previously recognized polyphenolic compounds, carbohydrates and steroids are also detected in the medulla of some Usnea species; ii) the use of sulfuric anisaldehyde reagent greatly improves the detection of terpenes, carbohydrates and steroids compared with the sulfuric acid reagent routinely used in thin layer chromatography; iii) among carbohydrates, we detected arabitol and sucrose in the medulla; iv) steroids and terpenes remain unidentified and deserve further investigations.

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Substitution of methyl tert.-butyl ether for diethyl ether in the standardized thin-layer chromatographic method for lichen products

Cited by 290 publications

References 7 publications

A molecular phylogeny of the Lecanora varia group, including a new species from western North America

A molecular phylogeny of the Lecanora varia group, including a new species from western North America

Notes on the Genus Usnea Dill. Ex Adanson

The Use of Anisaldehyde Sulfuric Acid as an Alternative Spray Reagent in TLC Analysis Reveals Three Classes of Compounds in the Genus <em>Usnea</em> Adans. (Parmeliaceae, lichenized Ascomycota)

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