Substituting Fish Meal with a Bacteria Protein (Clostridium autoethanogenum Protein) Derived from Industrial-Scale Gas Fermentation: Effects on Growth and Gut Health of Juvenile Large Yellow Croakers (Larimichthys crocea)
Abstract:This study was conducted to investigate the effects of Clostridiumautoethanogenum protein (CAP) replacement for fish meal (FM) on growth performance, digestive enzyme activity, humoral immunity and liver and intestinal health in large yellow croakers (Larimichthys crocea). Four experimental diets were formulated by replacing FM with CAP at different levels—0% (CAP0), 15% (CAP15), 30% (CAP30) and 45% (CAP45). Triplicate groups of juveniles (initial weight = 11.86 ± 0.13 g) were fed the test diets to apparent sa… Show more
“…1, which was found already with remarkable abundance in our previous study [5]. Members of this genus are normally found as part of the autochthonous gut microbiota in several sh species, such as rainbow trout [43], yellow cat sh (Pelteobagrus fulvidraco) [44], European sea bass [45], yellowtail king sh [46], large yellow croaker (Larimichthys crocea) [47], pearly razor sh (Xyrichtys novacula) and gilthead seabream [5]. Here, Ralstonia was present in all samples, but its abundance was clearly enhanced in sh fed with shrimps.…”
Changes in the gut microbial assemblages associated with dietary changes are still poorly explored, but may have profound effects in the life-history and physiology of fishes. Here, we have investigated the influence of the diet type on the autochthonous gut microbiomes of gilthead seabream (Sparus aurata). We conducted a 60-day assay in which a dietary shift from commercial to a diet based only on shrimp, followed by determination of gut microbial assemblages using 16S rRNA gene amplicon sequencing. The most prevalent species belonged to the genus Ralstonia, present in all samples, but was enhanced after the switch to shrimp diet. This seems to be a key genus whose functional relevance needs still to be unveiled given the wide distribution in samples with marine origin. Our results also pointed that nor the diet or the environmental microbiota (i.e. present in the water tanks) played a paramount role in the structure of the gut microbiome at the long-term. After 40 days, the gut microbial communities under both dietary treatments were very similar, being some species of the genera Ralstonia, Paraburkholderia, Fulvimonas, Pseudomonas and Cutibacteriumpart of the core-microbiome conserved at similar abundances over time. These results may challenge the common wisdom that diet is the primary determinant of gut microbiome composition in the long-term. In the era of the search for alternatives to fish-based ingredients in the aquafeed sector, and considering the paramount role of the gut microbiota in the fish health, this work offers valuable information for feed manufacturers to explore different nutritional strategies for aquaculture production, as long as the impact of the feeds on the physiology and quality of the fish are also tested.
“…1, which was found already with remarkable abundance in our previous study [5]. Members of this genus are normally found as part of the autochthonous gut microbiota in several sh species, such as rainbow trout [43], yellow cat sh (Pelteobagrus fulvidraco) [44], European sea bass [45], yellowtail king sh [46], large yellow croaker (Larimichthys crocea) [47], pearly razor sh (Xyrichtys novacula) and gilthead seabream [5]. Here, Ralstonia was present in all samples, but its abundance was clearly enhanced in sh fed with shrimps.…”
Changes in the gut microbial assemblages associated with dietary changes are still poorly explored, but may have profound effects in the life-history and physiology of fishes. Here, we have investigated the influence of the diet type on the autochthonous gut microbiomes of gilthead seabream (Sparus aurata). We conducted a 60-day assay in which a dietary shift from commercial to a diet based only on shrimp, followed by determination of gut microbial assemblages using 16S rRNA gene amplicon sequencing. The most prevalent species belonged to the genus Ralstonia, present in all samples, but was enhanced after the switch to shrimp diet. This seems to be a key genus whose functional relevance needs still to be unveiled given the wide distribution in samples with marine origin. Our results also pointed that nor the diet or the environmental microbiota (i.e. present in the water tanks) played a paramount role in the structure of the gut microbiome at the long-term. After 40 days, the gut microbial communities under both dietary treatments were very similar, being some species of the genera Ralstonia, Paraburkholderia, Fulvimonas, Pseudomonas and Cutibacteriumpart of the core-microbiome conserved at similar abundances over time. These results may challenge the common wisdom that diet is the primary determinant of gut microbiome composition in the long-term. In the era of the search for alternatives to fish-based ingredients in the aquafeed sector, and considering the paramount role of the gut microbiota in the fish health, this work offers valuable information for feed manufacturers to explore different nutritional strategies for aquaculture production, as long as the impact of the feeds on the physiology and quality of the fish are also tested.
“…Thus, it is crucial to find low-price and high-quality protein sources to replace FM. In this Special Issue, there is a published paper reporting a bacteria protein derived from industrial-scale gas fermentation [1]. Clostridium autoethanogenum protein (CAP) is a new type of microbial protein produced by the fermentation of Clostridium autoethanogenum with carbon monoxide from steel-making waste gas [2].…”
Section: Fish Nutrition and Physiologymentioning
confidence: 99%
“…Compared with traditional FM, CAP has a richer amino acid profile and contains a higher protein content, which gives it an inherent advantage as a protein ingredient. Zhang's study showed that dietary FM could be replaced by 15% CAP in feeds containing 40% FM without adversely affecting the growth of large yellow croakers and, to some extent, improving the immunity of the organism [1]. Besides, shrimp by-product (SBp) has been identified as a possible animal protein source by increasing shrimp production from captures and farms.…”
“…Researches on L. crocea and M. salmoides showed that sh size is one of the key factors decided the optimal dietary level of CAP within the same sh species. For example, Zhang et al (2022) proved that CAP could replace 15% of shmeal for L. crocea with initial weight of 11.86g, higher dietary CAP (30% and 45%) reduced the growth and humoral immunity. But for the L. crocea with initial weight of 189.9 g, CAP can replace up to 30% of dietary shmeal protein without negative effects on the growth performance, intestinal healthy condition and esh quality (Wu et al 2022).…”
Clostridium autoethanogenum protein (CAP) is an emerging protein source with several key advantages and it has investigated on juvenile turbot (9.13 g). However, it was indicated that fish growth stage is a decisive factor for CAP performance and the influence of CAP on turbot intestine health has not been reported. Thus, it is worth investigating the effects of CAP on turbot extensively. In the present study, a 67-day feeding experiment was conducted to evaluate the effects of CAP on turbot in the grow-out phase from growth performance, intestine and liver health. Six hundred and thirty turbot with an initial body weight of about 150 g were randomly divided into 6 groups, which were fed with fishmeal-based control diet or diet with CAP to replace 15%, 30%, 45%, 60% and 75% of fishmeal protein in control diet, respectively. From growth and feed utilization, the substitution level of CAP should no more than 30%, which was less than previous reported on juvenile turbot. The palatability of the feed was linearly reduced. Although CAP did not induce enteritis, it decreased the intestine villi height in middle and intestine villi density in distal intestine. Except for decreasing the size of hepatocytes by CAP at 45% or higher substitution levels, no other abnormality observed in liver histology. However, dietary CAP reduced the triglyceride and cholesterol levels in the plasma at 30% or higher levels and linearly down-regulated genes related to lipogenesis in liver. The genes related lipid transportation and oxidation were up-regulated by CAP at the substitution levels of 15–60%. The present work confirmed the safety of CAP in fish feed and suggested more work should be conducted before it widely used in aquafeed.
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