Substituted phenanthrenes with basic amino side chains: A new series of anti-breast cancer agents

Centchroman mediated apoptosis involves cross-talk between extrinsic/intrinsic pathways and oxidative regulation

“…Cell culture MCF-7/MDA MB-231 cells procured from the National Center for Cell Sciences, Pune, India, and cultured as reported previously by us (Nigam et al, 2008;Saxena et al, 1995;Shagufta et al, 2006;Gupta et al, 2006;Srivastava et al, 2006). Briefly, the cells were cultured in DMEM, pH 7.4 containing penicillin (100 μg/ml), streptomycin (200 μg/ml), gentamicin (60 μg/ml) supplemented with 10% FBS and 10 mM HEPES in a humidified 5% CO 2 incubator at 37°C.…”

Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines

“…As shown in Fig. 1 (1 and 2), substituted phenanthrenes with basic amino side chains have shown significant anticancer activity against breast cancer [11]. Similarly, the pyrrolo [9,10] phenanthrene molecules that effectively intercalate with DNA results in synergetic enhancement of DNA immobilization/cross linking [12].…”

“…Cell cycle arrest and apoptotic properties have also been evaluated. The reason for choosing MCF-7 cell line for our experimentation is that we have been interested in finding out the relationship between substituted phenanthrenes and breast anticancer activities as some of the phenanthrene derivatives are known to exhibit antiproliferative effect against breast cancer cells [11]. Moreover, the expression of cyclin D1 has shown to increase the proliferation rate of MCF-7 cells.…”

Centchroman induces G0/G1 arrest and Caspase-dependent Apoptosis involving Mitochondrial Membrane Depolarization in MCF-7 and MDA MB-231 Human Breast Cancer Cells

“…Activation of caspases with apoptosis associated nuclear derangements and internucleosomal DNA ladder confirmed that CC induces apoptosis in MCF-7 and MDA MB-231 cells irrespective of ER. These cells are routinely being cultured as reported previously by us (Saxena et al, 1995;Shagufta et al, 2006;Gupta et al, 2006;Srivastava et al, 2006). Briefly, the cells were cultured in DMEM, pH 7.4 containing Penicillin (100 U/ml), Streptomycin (100 μg/ml), Gentamicin (60 μg/ml) supplemented with 10% FCS and 10 mM HEPES in a humidified 5% CO 2 incubator at 37°C.…”