Substantia Gelatinosa of the Spinal Cord

Ribeiro‐da‐Silva, Alfredo

doi:10.1016/b978-0-12-374245-2.00007-3

Cited by 9 publications

(2 citation statements)

References 131 publications

(170 reference statements)

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“…These populations also differ in their central termination in the spinal cord. Indeed, the peptidergic afferents terminate mostly in lamina I and outer lamina II, while the non-peptidergic project mostly to inner lamina II [ 1 , 8 – 10 ]. A considerable number of peptidergic afferents, as well as a few non-peptidergic afferents terminate in contact with lamina I projection neurons [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…In rat, a majority of spinoparabrachial neurons were shown to respond to noxious stimuli [ 19 , 20 ], and a high percentage of lamina I pyramidal projection neurons were considered NK-1r immunoreactive by another group [ 11 , 21 , 22 ]. However, our laboratory has consistently found that in naïve rats only about 22 % of the pyramidal neurons express the NK-1r [ 8 , 23 ]. Interestingly, we found that the percentage of pyramidal neurons expressing the NK-1r increased drastically in animal models of neuropathic and arthritis pain [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Limited Changes in Spinal Lamina I Dorsal Horn Neurons following the Cytotoxic Ablation of Non-Peptidergic C-Fibers

2015

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BackgroundNon-peptidergic nociceptive neurons are a sub-population of small diameter primary sensory neurons that comprise approximately 50 % of the C fiber population. Together with the peptidergic sub-population, they transmit nociceptive information from the periphery to the superficial dorsal horn of the spinal cord. Despite the numerous studies investigating the role of the non-peptidergic primary afferents, their role in normal nociception and in pain remains poorly understood. Our lab has previously demonstrated that, in rat models of neuropathic and inflammatory pain, there is a de novo expression of substance P receptors (NK-1r) by lamina I pyramidal projection neurons, a neuronal population that normally does not express these receptors.ResultsIn this study, we used a ribosomal toxin, saporin, conjugated to the lectin IB4 to selectively ablate the non-peptidergic nociceptive C fibers, to investigate if the loss of these fibers was enough to induce a change in NK-1r expression by lamina I projection neurons. IB4-saporin treatment led to the permanent ablation of the IB4-positive afferents but also to a small non-significant reduction in CGRP-positive afferents. An overall increase in immunoreactivity for the NK-1r was observed in lamina I projection neurons, however, the lack of non-peptidergic afferents did not increase the number of lamina I pyramidal projection neurons immunoreactive for the receptor.ConclusionsOur results demonstrate that the deletion of the non-peptidergic afferents, at the L4–L5 spinal levels, is not sufficient to trigger the de novo expression of NK-1r by projection pyramidal neurons but increases the expression of NK-1r in fusiform and multipolar projection neurons. Furthermore, our data suggest that a neuropathic component is essential to trigger the expression of NK-1r by pyramidal neurons.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Limited Changes in Spinal Lamina I Dorsal Horn Neurons following the Cytotoxic Ablation of Non-Peptidergic C-Fibers

2015

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Convergence of peptidergic and non‐peptidergic protein markers in the human dorsal root ganglion and spinal dorsal horn

et al. 2021

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Peripheral sensory neurons are characterized by their size, molecular profiles, and physiological responses to specific stimuli. In mouse, the peptidergic and non‐peptidergic subsets of nociceptors are distinct and innervate different lamina of the spinal dorsal horn. The unique molecular signature and neuroanatomical organization of these neurons supports a labeled line theory for certain types of nociceptive stimuli. However, long‐standing evidence supports the polymodal nature of nociceptors in many species. We have recently shown that the peptidergic marker, CGRP, and the non‐peptidergic marker, P2X3R, show largely overlapping expression at the mRNA level in human dorsal root ganglion (DRG). Herein, our aim was to assess the protein distribution of nociceptor markers, including their central projections, in the human DRG and spinal cord. Using DRGs obtained from organ donors, we observed that CGRP and P2X3R were co‐expressed by approximately 33% of human DRG neurons and TrpV1 was expressed in ~60% of human DRG neurons. In the dorsal spinal cord, CGRP, P2X3R, TrpV1, and Nav1.7 proteins stained the entirety of lamina 1–2, with only P2XR3 showing a gradient of expression. This was confirmed by measuring the size of the substantia gelatinosa using Hematoxylin and Eosin staining of adjacent sections. Our findings are consistent with the known polymodal nature of most primate nociceptors and indicate that the central projection patterns of nociceptors are different between mice and humans. Elucidating how human nociceptors connect to subsets of dorsal horn neurons will be important for understanding the physiological consequences of these species differences.

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Cross Talk of BDNF and GDNF in Spinal Substantia Gelatinosa (Lamina II): Focus on Circuitry

Merighi

2021

Advances in Experimental Medicine and Biology

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Substantia Gelatinosa of the Spinal Cord

Cited by 9 publications

References 131 publications

Limited Changes in Spinal Lamina I Dorsal Horn Neurons following the Cytotoxic Ablation of Non-Peptidergic C-Fibers

Limited Changes in Spinal Lamina I Dorsal Horn Neurons following the Cytotoxic Ablation of Non-Peptidergic C-Fibers

Convergence of peptidergic and non‐peptidergic protein markers in the human dorsal root ganglion and spinal dorsal horn

Cross Talk of BDNF and GDNF in Spinal Substantia Gelatinosa (Lamina II): Focus on Circuitry

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