Substances for regenerative wound healing during antler renewal stimulated scar-less restoration of rat cutaneous wounds

Guo, Qianqian; Liu, Zhen; Zheng, Jiong; Zhao, Haiping; Li, Chunyi

doi:10.1007/s00441-021-03505-9

Cited by 7 publications

(9 citation statements)

References 60 publications

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“…In this study, we found that AnSCs had more potent mitogenic effects in vitro and effects on animal survival and reduction of PF symptoms in the mouse model in vivo than other types of MSCs, probably because AnSCs possess partial ESC properties [ 21 , 42 , 43 ]. Consistently, we have found highly potent anti-fibrotic effects of AnSCs in previous studies, including regenerative wound healing (reduction of scaring) and reduction of liver fibrogenesis [ 14 , 26 , 44 ].…”

Section: Discussionsupporting

confidence: 87%

Antler stem cell exosomes alleviate pulmonary fibrosis via inhibiting recruitment of monocyte macrophage, rather than polarization of M2 macrophages in mice

Zhang,

Shi,

et al. 2023

Cell Death Discov.

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Pulmonary fibrosis (PF), a chronic interstitial lung disease, is characterized by over-abundant deposition of extracellular matrix consisting mainly of collagen I. In previous studies, we demonstrated that deer antler stem cells (AnSCs), a novel type of adult stem cell, are capable of significantly down-regulating collagen formation in different organs and tissues and speculated that they could effectively treat PF via reducing collagen deposition in the lung tissue. In the present study, we found that administration of AnSCs improved the survival rate of PF mice and reduced lung fibrosis, collagen deposition and myofibroblast differentiation. The effects of AnSC treatment were significantly better than the positive control (adipose-derived stem cells). Interestingly, AnSC-Exos were almost equally effective as AnSCs in treating PF, suggesting that the effects of AnSCs on reduction of PF may be mainly through a paracrine mechanism. Further, AnSC-Exos reduced the number of M2 macrophages, a type of macrophage that secrets pro-fibrotic factors to accelerate fibrotic progression, in the lung tissues. In vitro experiments showed that the effects of AnSC-Exos on macrophage modulation were likely achieved via inhibition of the recruitment of circulating monocyte-derived macrophages (reducing the number of macrophages), rather than via inhibition of M2 polarization of macrophages. Inhibition of macrophage recruitment by AnSCs may be achieved indirectly via inhibiting CCL7 expression in fibroblasts; both let-7b and let-7a were highly enriched in AnSC-Exos and may play a critical role in the inhibition of CCL7 expression of fibroblasts. Collectively, the use of antler stem cells or their exosomes opens up a novel strategy for PF treatment in the clinical setting.

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Section: Discussionsupporting

confidence: 87%

Antler stem cell exosomes alleviate pulmonary fibrosis via inhibiting recruitment of monocyte macrophage, rather than polarization of M2 macrophages in mice

Zhang,

Shi,

et al. 2023

Cell Death Discov.

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“…After the successful preparation of the diabetes model, surgical scissors were employed to create a full-thickness excisional wound measuring 0.8 cm × 0.8 cm on the dorsal skin of each mouse [ 51 ]. Three cohorts of mice, each comprising six animals, were grouped at random.…”

Section: Methodsmentioning

confidence: 99%

Hair follicle mesenchymal stem cell exosomal lncRNA H19 inhibited NLRP3 pyroptosis to promote diabetic mouse skin wound healing

Yang

Zhang

et al. 2023

Aging

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Skin wounds caused by diabetes are a major medical problem. Mesenchymal stem cell-derived exosomes hold promise to quicken wound healing due to their ability to transfer certain molecules to target cells, including mRNAs, microRNAs, lncRNAs, and proteins. Nonetheless, the specific mechanisms underlying this impact are not elucidated. Therefore, this research aimed to investigate the effect of MSC-derived exosomes comprising long non-coding RNA (lncRNA) H19 on diabetic skin wound healing. Hair follicle mesenchymal stem cells (HF-MSCs) were effectively isolated and detected, and exosomes (Exo) were also isolated smoothly. Pretreatment with 30 mM glucose for 24 h (HG) could efficiently induce pyroptosis in HaCaT cells. Exosomal H19 enhanced HaCaT proliferation and migration and inhibited pyroptosis by reversing the stimulation of the NLRP3 inflammasome. Injection of exosomes overexpressing lncRNA H19 to diabetic skin wound promoted sustained skin wound healing, whereas sh-H19 exosomes did not have this effect. In conclusion, Exosomes overexpressing H19 promoted HaCaT proliferation, migration and suppressed pyroptosis both in vitro and in vivo . Therefore, HFMSC-derived exosomes that overexpress H19 may be included in strategies for healing diabetic skin wounds.

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“…Deer antlers are the only mammalian appendage that, once lost, can fully regenerate 21–23 . Antler regeneration relies on the periosteum of the pedicle (PP), a bony protuberance permanently covered by skin from which antlers are cast and regenerate annually 24,25 . Each year, around 3.3 million PP cells give rise to up to 15 kg of antler tissue mass in around 3 months 26 .…”

Section: Introductionmentioning

confidence: 99%

“…[21][22][23] Antler regeneration relies on the periosteum of the pedicle (PP), a bony protuberance permanently covered by skin from which antlers are cast and regenerate annually. 24,25 Each year, around 3.3 million PP cells give rise to up to 15 kg of antler tissue mass in around 3 months. 26 As PP cells have stem cell attributes, they have been termed antler stem cells (AnSCs).…”

Section: Introductionmentioning

confidence: 99%

Conditioned media of deer antler stem cells accelerate regeneration of alveolar bone defects in rats

Guo¹,

Zheng

Lin

et al. 2023

Cell Proliferation

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The destruction of periodontal alveolar bone (AB) caused by periodontitis is regarded as one of the major reasons for tooth loss. The inhibition of bone resorption and regeneration of lost AB are the desirable outcomes in clinical practice but remain in challenge. The use of mesenchymal stem cells (MSCs) is one current approach for achieving true restoration of AB defects (ABD). Antler stem cells (AnSC) are capable of renewing a huge mammalian bony appendage, the deer antler, suggesting an unparalleled potential for bone regeneration. Herein, we investigated the effectiveness of deer AnSCs conditioned medium (CM, AnSC-CM) for repair of surgically-created ABD using a rat model and sought to define the underlying mechanisms. The results showed that AnSC-CM effectively induced regeneration of AB tissue; the outcome was significantly better than human bone marrow mesenchymal stem cell conditioned medium (hBMSC-CM). AnSC-CM treatment upregulated osteogenic factors and downregulated osteoclastic differentiation factors; stimulated proliferation, migration and differentiation of resident MSCs toward osteogenic lineage cells; modulated macrophage polarization toward the M2 phenotype and suppressed osteoclastogenesis. That AnSC-CM resulted in better outcomes than hBMSC-CM in treating ABD was attributed to the cell compatibility as both AnSCs and AB tissue are neural crest-derived. In conclusion, the effects of AnSC-CM on AB tissue regeneration were achieved through both promotion of osteogenesis and inhibition of osteoclastogenesis. We believe that AnSC-CM is a candidate for effective treatment of ABD in dental clinical practice but will require investment in further development.

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Substances for regenerative wound healing during antler renewal stimulated scar-less restoration of rat cutaneous wounds

Cited by 7 publications

References 60 publications

Antler stem cell exosomes alleviate pulmonary fibrosis via inhibiting recruitment of monocyte macrophage, rather than polarization of M2 macrophages in mice

Antler stem cell exosomes alleviate pulmonary fibrosis via inhibiting recruitment of monocyte macrophage, rather than polarization of M2 macrophages in mice

Hair follicle mesenchymal stem cell exosomal lncRNA H19 inhibited NLRP3 pyroptosis to promote diabetic mouse skin wound healing

Conditioned media of deer antler stem cells accelerate regeneration of alveolar bone defects in rats

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