Substance P stimulates late-stage rat osteoblastic bone formation through neurokinin-1 receptors

Goto, Tetsuya; Nakao, Kayoko; Gunjigake, Kaori; Kido, Mizuho A.; Kobayashi, Shigeru; Takano, Teruo

doi:10.1016/j.npep.2006.11.002

Cited by 85 publications

(74 citation statements)

References 23 publications

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“…Many of these genes affect functions that are important in the periodontium. For example, NPY and substance P, a TAC1 gene product, can enhance osteoblast differentiation (Goto et al, 2007;Franquinho et al, 2010). Thus, mRNA levels of neuron-associated genes are affected by diabetes-enhanced inflammation, which may have important ramifications in the periodontium.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Dynamics during Diabetic Periodontitis

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Gene Expression Dynamics during Diabetic Periodontitis

et al. 2012

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“…In support of these findings, aggrecan shows increased mRNA transcription at the fused stage. Aggrecan has been detected in the cartilaginous matrix surrounding hypertrophic chondrocytes in ossification centers in mammalian vertebrae (Smith et al 2009), and the increased transcription of osteogenic markers has been reported in mammalian SP-treated osteoblast-like cultures (Sun et al 2010;Goto et al 2007). The expression of osteogenic markers, such as runx2, col1a, and osteocalcin, has been detected in hyperdense notochordal cells, together with chondrogenic marker genes such as sox9 and col2 (Ytteborg et al 2010a).…”

Section: Discussionmentioning

confidence: 99%

“…Perlecan has structural roles in mammalian cartilage and IVD (Sivan et al 2006) and is important for the proper establishment of basement membranes in various vertebrates including teleosts (Parsons et al 2002;Aviezer et al 1994). Furthermore, Substance P (SP), which has been established as a marker for neuronal invasion in both mammals and teleosts (Holmqvist and Ekstrom 1991;Hökfelt et al 1975), also has important roles in mineralization processes of both osteoblasts (Sun et al 2010;Goto et al 2007) and chondrocytes (Millward-Sadler et al 2003). Hence, immunohistochemical markers for ECM components, mineralization, and neuronal development, such as collagen, Aggrecan, Perlecan, and SP, have been used to characterize pathological vertebral conditions (Kuraishi et al 1985;Hökfelt et al 1975).…”

Section: Introductionmentioning

confidence: 99%

Remodeling of the notochord during development of vertebral fusions in Atlantic salmon (Salmo salar)

et al. 2010

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Histological characterization of spinal fusions in Atlantic salmon (Salmo salar) has demonstrated shape alterations of vertebral body endplates, a reduced intervertebral space, and replacement of intervertebral cells by ectopic bone. However, the significance of the notochord during the fusion process has not been addressed. We have therefore investigated structural and cellular events in the notochord during the development of vertebral fusions. In order to induce vertebral fusions, Atlantic salmon were exposed to elevated temperatures from fertilization until they attained a size of 15g. Based on results from radiography, intermediate and terminal stages of the fusion process were investigated by immunohistochemistry and real-time quantitative polymerase chain reaction. Examination of structural extracellular matrix proteins such as Perlecan, Aggrecan, Elastin, and Laminin revealed reduced activity and reorganization at early stages in the pathology. Staining for elastic fibers visualized a thinner elastic membrane surrounding the notochord of developing fusions, and immunohistochemistry for Perlecan showed that the notochordal sheath was stretched during fusion. These findings in the outer notochord correlated with the loss of Aggrecan- and Substance-P-positive signals and the further loss of vacuoles from the chordocytes in the central notochord. At more progressed stages of fusion, chordocytes condensed, and the expression of Aggrecan and Substance P reappeared. The hyperdense regions seem to be of importance for the formation of notochordal tissue into bone. Thus, the remodeling of notochord integrity by reduced elasticity, structural alterations, and cellular changes is probably involved in the development of vertebral fusions.

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“…Peptidergic sensory fibers containing CGRP and/or SP have been found in bone [9,10]. SP is a neuropeptide in sensory neurons that acts on the neurokinin-1 receptor, by which it activates both osteoclasts and osteoblasts in vitro [42,43]. However, mice in which the gene for SP has been deleted are reported to have normal bone [44], although a detailed description of bone phenotypes in this strain has not been reported.…”

Section: Possible Neuropeptide Mediators For Sensory Fiber Action(s) mentioning

confidence: 99%

Evolving Concepts in Neurogenic Osteoporosis

Qin

Bauman

Cardozo

2010

Curr Osteoporos Rep

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Convincing evidence has accumulated of regulation of bone by the central nervous system. The neural connection between brain and bone is mediated centrally by classic neurotransmitters and several neuropeptides, and peripherally by many of the same neurotransmitters and neuropeptides, albeit with actions opposite to their central effects. Pharmacologic blockade of ß2-adrenergic receptors or disruption of the gene encoding them increases bone mass, whereas increased activity of the sympathetic nervous system (SNS) contributes to bone loss. Brainstem serotonergic neurons regulate SNS activity and its modulation by leptin. Physiologic stimulation of osteoblastic nicotinic receptors results in proliferation and deposition of bone, whereas higher levels inhibit osteoblast function. Activation of sensory nerves has a centrally mediated action on bone, albeit poorly understood. The relative importance of, and interactions between autonomic, sensory, and peripheral nervous system actions on bone mass are also not clear in healthy individuals, and less so in pathologic states.

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Substance P stimulates late-stage rat osteoblastic bone formation through neurokinin-1 receptors

Cited by 85 publications

References 23 publications

Gene Expression Dynamics during Diabetic Periodontitis

Gene Expression Dynamics during Diabetic Periodontitis

Remodeling of the notochord during development of vertebral fusions in Atlantic salmon (Salmo salar)

Evolving Concepts in Neurogenic Osteoporosis

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