Substance P in peritoneal fluid

Sanfilippo, Joseph S.; Williams, Rosie; Yussman, Marvin A.; Cook, Christine L.; Bissonnette, François

doi:10.1016/0002-9378(92)91852-2

Cited by 23 publications

(13 citation statements)

References 2 publications

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“…Substance P (SP) is a member of the tachykinin family and a strong mediator of neurogenic inflammation [82]. It is produced and secreted by afferent sensory nerves, and has been detected in the peritoneum, close to endometriotic lesions [52,57], as well as in DIE and rectal lesions [58], and in the PF [83].…”

Section: Neurogenic Inflammation and Endometriotic Progressionmentioning

confidence: 99%

Inflammation and nerve fiber interaction in endometriotic pain

McKinnon

Bertschi

Bersinger

et al. 2015

Trends in Endocrinology & Metabolism

169

130

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Section: Neurogenic Inflammation and Endometriotic Progressionmentioning

confidence: 99%

Inflammation and nerve fiber interaction in endometriotic pain

McKinnon

Bertschi

Bersinger

et al. 2015

Trends in Endocrinology & Metabolism

169

130

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“…Neurons are the primary source of SP, but lymphocytes (29), monocytes (30), macrophages (30), and eosinophils (31) have been identified as additional sources. SP is present in peritoneal fluid (32), and SP-containing sensory neurons have been found in peritoneal adhesions (33).…”

mentioning

confidence: 99%

A neurokinin 1 receptor antagonist decreases postoperative peritoneal adhesion formation and increases peritoneal fibrinolytic activity

Reed

Fruin

Gower

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Fibrous adhesions remain a major sequela of abdominal surgery. The proinflammatory peptide substance P (SP), known to participate in inflammatory events, may play a key role in adhesion formation. This hypothesis was tested by using an antagonist, CJ-12,255 (Pfizer), that blocks the binding of SP to the neurokinin 1 receptor (NK-1R). Adhesion formation was surgically induced in the peritoneum of rats receiving daily doses of the NK-1R antagonist (NK-1RA; 5.0 or 10.0 mg͞kg per day) or saline. On postoperative day 7, both the low and high doses of NK-1RA significantly (P < 0.05) reduced adhesion formation by 45% and 53%, respectively, compared with controls. Subsequently, the effect of NK-1RA administration on peritoneal fibrinolytic activity was investigated to determine a potential mechanism for SP action in the peritoneum. Samples were collected from nonoperated controls and from animals 24 h postsurgery that were administered either NK-1RA or saline. Fibrinolytic activity in peritoneal fluid was assayed by zymography, and expression of tissue plasminogen activator (tPA) and plasminogen activator inhibitor 1, both regulators of fibrinolytic activity, was assessed in peritoneal tissue and fluid by RT-PCR and bioassay, respectively. NK-1RA administration led to a marked (P < 0.05) increase in tPA mRNA levels in peritoneal tissue compared with nonoperated and saline-administered animals. Likewise, NK-1RA administration significantly (P < 0.05) increased tPA in the peritoneal fluid. These data suggest that activation of the NK-1R promotes peritoneal adhesion formation by limiting fibrinolytic activity in the postoperative peritoneum, thus enabling fibrinous adhesions to persist.

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“…This antagonist has also been shown to suppress mRNA expression of ICAM-1 in mouse lung during acute pancreatitis (30). A potential role for activation of the NK-1R in adhesion formation is further supported by the fact that substance P is present in peritoneal fluid (43), substance P and NK-1R mRNA levels increase in peritoneal tissue following abdominal operation (40), and substance P-containing sensory neurons have been demonstrated in peritoneal adhesions (50).…”

mentioning

confidence: 93%

A neurokinin-1 receptor antagonist that reduces intra-abdominal adhesion formation decreases oxidative stress in the peritoneum

Reed¹,

Heydrick²,

Aarons³

et al. 2007

American Journal of Physiology-Gastrointestinal and Liver Physi

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Oxidative stress has been implicated in intra-abdominal adhesion formation. Substance P, a neurokinin-1 receptor (NK-1R) ligand, facilitates leukocyte recruitment and reactive oxygen species (ROS) generation. We have shown in a rat model of adhesion formation that intraperitoneal administration of a NK-1R antagonist at the time of abdominal operation reduces postoperative adhesion formation. Thus we determined the effects of NK-1R antagonist administration on peritoneal leukocyte recruitment and oxidative stress within 24 h of surgery. Adhesions were induced in Wistar rats randomly assigned to receive the antagonist or vehicle intraperitoneally. Peritoneal tissue was isolated at 2, 4, 6, and 24 h after surgery for analysis of the oxidative stress biomarkers 8-isoprostane (8-IP), protein carbonyl, NADPH oxidase, myeloperoxidase (MPO), and ICAM-1 and VCAM-1 mRNAs. Total antioxidant capacity of peritoneal fluid was also determined. MPO, NADPH oxidase, 8-IP, and protein carbonyl were elevated (P < 0.05) by 6 h. ICAM-1 mRNA was elevated (P < 0.05) by 2 h, whereas VCAM-1 levels decreased (P < 0.05) at 24 h. The NK-1R antagonist delayed the MPO rise and reduced (P < 0.05) 8-IP levels by 6 h and ICAM-1 mRNA, VCAM-1 mRNA, and protein carbonyl at 2 h. The antagonist also increased (P < 0.05) the antioxidant capacity of peritoneal fluid at all time points. These data further support a role for oxidative stress in adhesion formation and suggest that the NK-1R antagonist may limit adhesions, in part, by reducing postoperative oxidative stress through an inhibition of neutrophil recruitment and an increase in peritoneal fluid antioxidant capacity.

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Substance P in peritoneal fluid

Cited by 23 publications

References 2 publications

Inflammation and nerve fiber interaction in endometriotic pain

Inflammation and nerve fiber interaction in endometriotic pain

A neurokinin 1 receptor antagonist decreases postoperative peritoneal adhesion formation and increases peritoneal fibrinolytic activity

A neurokinin-1 receptor antagonist that reduces intra-abdominal adhesion formation decreases oxidative stress in the peritoneum

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