Substance P in cardiovascular diseases – A bioanalytical review

Feickert, Martin; Burckhardt, Bjoern B.

doi:10.1016/j.cca.2019.05.014

Cited by 18 publications

(10 citation statements)

References 56 publications

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“…In particular, SP influences cardiomyocyte activity through the activation of cholinergic innervation and causes bradycardia [55,56]. Moreover, SP regulates the blood flow in vessels located in the heart wall (the character of this impact depends on gender) [57,58] and may stimulate the growth of cardiomyocytes [59] and influence the heart mast cells, stimulating the release of pro-inflammatory factors [60][61][62]. It is also known that SP participates in mechanisms connected with various heart diseases, including viral and parasitic infections, heart ischemia and cardiac fibrosis [54,59].…”

Section: Discussionmentioning

confidence: 99%

Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart

Makowska

Gonkowski

2021

Animals

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Bisphenol A (BPA) contained in plastics used in the production of various everyday objects may leach from these items and contaminate food, water and air. As an endocrine disruptor, BPA negatively affects many internal organs and systems. Exposure to BPA also contributes to heart and cardiovascular system dysfunction, but many aspects connected with this activity remain unknown. Therefore, this study aimed to investigate the impact of BPA in a dose of 0.05 mg/kg body weight/day (in many countries such a dose is regarded as a tolerable daily intake–TDI dose of BPA–completely safe for living organisms) on the neurochemical characterization of nerves located in the heart wall using the immunofluorescence technique. The obtained results indicate that BPA (even in such a relatively low dose) increases the number of nerves immunoreactive to neuropeptide Y, substance P and tyrosine hydroxylase (used here as a marker of sympathetic innervation). However, BPA did not change the number of nerves immunoreactive to vesicular acetylcholine transporter (used here as a marker of cholinergic structures). These observations suggest that changes in the heart innervation may be at the root of BPA-induced circulatory disturbances, as well as arrhythmogenic and/or proinflammatory effects of this endocrine disruptor. Moreover, changes in the neurochemical characterization of nerves in the heart wall may be the first sign of exposure to BPA.

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Section: Discussionmentioning

confidence: 99%

Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart

Makowska

Gonkowski

2021

Animals

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“…The discrepancy between these failures and promising preclinical studies leads to the assumption that the roles of the tachykinins are not fully understood until now [5]. This lack of knowledge may be based on the high variability of SP blood levels caused by distinct analytical techniques and sample preparation methods [6][7][8]. The determination of SP and HK-1 blood levels are predominantly executed by immunoassays, which often lack the required selectivity to differentiate between SP and HK-1 due to crossreactivity [9,10].…”

Section: Rpkpqqffglm-nh2mentioning

confidence: 99%

“…As SP and HK-1 are positively charged and moderately hydrophobic peptides (Table 1), they unify the potential to adsorb to glass as well as plastic surfaces [14][15][16]. Due to their low concentrations in picomolar ranges in plasma samples [8], adsorption processes are highly critical and play an essential role during accurate sample preparation and measurement. Moreover, the phenomenon of adsorption may lead to an underestimation of peptide levels in the case of biomarker assays and subsequently results in incorrect conclusions of biomarker levels and poor comparability between different studies [17,18].…”

Section: Rpkpqqffglm-nh2mentioning

confidence: 99%

“…SP plasma levels were significantly distinct between diseased and healthy subjects, and therefore could be a potential biomarker to support a diagnostic or monitor the progress of pathological events. However, measured SP plasma levels showed high variability between clinical studies [8]. Variability was known to be caused by distinct analytical methods and different sampling procedures.…”

Section: Reliability Of Determined Substance P Levelsmentioning

confidence: 99%

“…This lack of knowledge may be based on the high variability of SP blood levels caused by distinct analytical techniques and sample preparation methods . The determination of SP and HK‐1 blood levels are predominantly executed by immunoassays, which often lack the required selectivity to differentiate between SP and HK‐1 due to cross‐reactivity .…”

Section: Introductionmentioning

confidence: 99%

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A design of experiments concept for the minimization of nonspecific peptide adsorption in the mass spectrometric determination of substance P and related hemokinin‐1

Feickert

Burckhardt

2019

J of Separation Science

Self Cite

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Substance P and hemokinin‐1 were predominantly examined by immunoassays with their limitation to differentiate appropriately between both peptides. The use of liquid chromatography coupled with tandem mass spectrometry is a promising, highly selective alternative. Adsorption processes have been identified in preliminary experiments to play a crucial role in the loss of mass spectrometry intensity of both peptides. Therefore, a design of experiments concept was created to minimize nonspecific peptide adsorption. For this purpose, the most critical influencing parameters—(1) the composition of the injection solvent as well as (2) the most suitable container material—were systematically and concordantly investigated. The addition of modifiers, such as formic acid, dimethyl sulfoxide, and organic solvents, to the injection solvent led to a substantial gain of intensity of substance P and hemokinin‐1 compared to the start gradient as an injection solvent. Furthermore, the systematic investigation underlined the high impact of the container material, demonstrating polypropylene as the most favorable material. A conjoint injection solvent optimum was found to determine both peptides simultaneously by the conduction of a sweet‐spot analysis. The experimental design substantially reduced nonspecific peptide adsorption and enabled the simultaneous and selective determination of endogenous substance P and hemokinin‐1 plasma levels.

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Prevention of tick-borne diseases: challenge to recent medicine

Hromníková

Furka

et al. 2022

Biologia

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Ticks represent important vectors and reservoirs of pathogens, causing a number of diseases in humans and animals, and significant damage to livestock every year. Modern research into protection against ticks and tick-borne diseases focuses mainly on the feeding stage, i.e. the period when ticks take their blood meal from their hosts during which pathogens are transmitted. Physiological functions in ticks, such as food intake, saliva production, reproduction, development, and others are under control of neuropeptides and peptide hormones which may be involved in pathogen transmission that cause Lyme borreliosis or tick-borne encephalitis. According to current knowledge, ticks are not reservoirs or vectors for the spread of COVID-19 disease. The search for new vaccination methods to protect against ticks and their transmissible pathogens is a challenge for current science in view of global changes, including the increasing migration of the human population. Highlights • Tick-borne diseases have an increasing incidence due to climate change and increased human migration • To date, there is no evidence of transmission of coronavirus COVID-19 by tick as a vector • To date, there are only a few modern, effective, and actively- used vaccines against ticks or tick-borne diseases • Neuropeptides and their receptors expressed in ticks may be potentially used for vaccine design

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Substance P in cardiovascular diseases – A bioanalytical review

Cited by 18 publications

References 56 publications

Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart

Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart

A design of experiments concept for the minimization of nonspecific peptide adsorption in the mass spectrometric determination of substance P and related hemokinin‐1

Prevention of tick-borne diseases: challenge to recent medicine

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