Substance P in brain extracts

Cleugh, Joan; Gaddum, J. H.; Mitchell, Allen A.; Smith, M. W.; Whittaker, V. P.

doi:10.1113/jphysiol.1964.sp007314

Cited by 48 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 70 % methanol fraction was particularly active in causing contraction of the rat uterus; this has been shown before (Gaddum, 1961;Cleugh et al, 1964). When this fraction was injected into six mice, it showed no antistrychnine activity; in fact the mean time to the first convulsion following the injection of strychnine was actually shortened, but this was not significant (P > 0-05, Table 2).…”

Section: Resultsmentioning

confidence: 73%

“…Doses were given every 5 min. Since the rat uterus is very insensitive to pure SP (Cleugh et al 1964) the effect of the extracts is thought to be mainly due to other unknown substances. These substances were monitored to give some indication of the purity of the new laboratory standard of SP and to see whether the material contracting the rat uterus might have an antistrychnine effect.…”

Section: Methodsmentioning

confidence: 99%

“…The guinea-pig ileum contracts in the presence of several substances extracted from horse intestine (Zetler, 1963), but the main effect is due to SP. This preparation is considered to be the most suitable for the assay of SP (Cleugh, Gaddum, Mitchell, Smith & Whittaker, 1964). It was used at a temperature of 360C.…”

Section: Methodsmentioning

confidence: 99%

“…During the preparation of the laboratory standard, extracts of horse intestine were assayed against the unofficial standard of SP prepared by Messrs Hoffmann-La Roche and referred to as IS by Cleugh et al (1964). This preparation had, by definition, 75 u. of activity per mg when assayed on the guinea-pig ileum or rat uterus.…”

Section: Standardsmentioning

confidence: 99%

See 3 more Smart Citations

An antistrychnine extract from horse intestine

Gaddum¹,

Randić²,

Smith³

1964

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Standardsmentioning

confidence: 99%

See 2 more Smart Citations

An antistrychnine extract from horse intestine

Gaddum¹,

Randić²,

Smith³

1964

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

“…The two final products behaved differently when subjected to gel filtration and electrodialysis, but both were inactivated by incubation with chymotrypsin. 66P PHYSIOLOGICAL SOCIETY, 14 JANUARY 1967 Assay was performed on the terminal ileum of the guinea-pig in Krebs Ringer (Cleugh, Gaddum, Mitchell, Smith & Whittaker, 1964) We have measured the amount of 22Na which passes from the extracellular fluid into the uterine lumen during a half-hour perfusion period, at different stages of the sexual cycle. Our main interest was in the preimplantation and early post-implantation stages of pregnancy when the blastocyst relies on the uterine secretion for its nutrition.…”

Section: Pmentioning

confidence: 99%

Proceedings of the Physiological Society, 14 January 1967; Guy's Hospital Meeting: Communications

1967

The Journal of Physiology

View full text Add to dashboard Cite

The accumulation of noradrenaline (NA) proximal to a single constriction in sympathetic nerves has been attributed to its proximo-distal movement along the axons (Kapeller & Mayor, 1966a, b). This movement has been investigated further after constricting guinea-pig and cat hypogastric nerves at two points, 0-5-2 cm apart, with either fine forceps or a fine silk ligature. Whole mount preparations of the guinea-pig nerves and 6-10 ,c sections of the cat nerves were examined by the fluorescence microscopic method for NA at intervals up to 3 days after operation. Some cat nerves were also studied with the electron microscope.Proximal to the proximal constriction there was a rapid accumulation of fluorescent material in fibres which became progressively more swollen. This was first seen 15 min after operation and was still evident after 3 days. A similar, but much smaller, accumulation was seen proximal to the distal constriction. It was less intense and affected a shorter segment of nerve than the accumulation proximal to the proximal constriction. It disappeared between 24 and 48 hr after operation.Immediately distal to the constrictions the nerves gave a diffuse yellowish fluorescence, but in general showed no specific greenish NA fluorescence. Occasionally nerve fibres which exhibited the typical NA fluorescence have been found distal to the constrictions. These were seen more often beyond the distal lesion. Almost invariably, in the guinea-pig nerves, these fibres could be traced to a group ofhighly fluorescent ganglion cells situated more distally.With the electron microscope an accumulation of granular vesicles, believed to carry NA, has been found proximal to both constrictions. This was more marked in relation to the proximal than to the distal constriction. No such accumulation has so far been found distal to either lesion. This corresponds to the behaviour of the fluorescent material in these nerves.These findings support the view that NA travels proximo-distally along sympathetic nerves. Since an accumulation of NA also occurs proximal to

show abstract

Tritium labelling of substance P and the corresponding N‐terminal 1‐7 fragment in the pro², pro⁴ positions

Stewart

Sundqvist

Chang

et al. 1983

Labelled Comp Radiopharmac

View full text Add to dashboard Cite

h e d o b s e r v a t i o n ) . R e d u c t i v e 8 d e h a l o g e n a t i o n of p a r a -s u b s t i t u t e d phenyl g r o u p s ( P h e 7 and Phe h a s a l s o been u s e d ( 2 -4 ) . Hanley and c o -w o r k e r s ( 4 ) r e p o r t good s p e c i f i c a c t i v i t y ( 2 3 Ci/mmol) o f s u b s t a n c e P l a b e l l e d i n t h e Phe p o s i t i o n and B e r g e r e t a1 ( 2 ) t h e N l e ' l -s u b s t a n c e P a n a l o g u e , l a b e l l e d a t Phe7 and P h e 8 t o 27 Ci/mmol.8 W e have used t h e a l t e r n a t i v e a p p r o a c h ( 5 ) o f i n t r o d u c i n g t r i t i u m by c a t a l y t i c h y d r o g e n a t i o n of t h e u n s a t u r a t e d p r e c u r s o r s w i t h 3 , 4 dehydro-Pro', 3 , 4 d e h y d r o -P r o 4 s u b s t i t u t i o n . The t r i t i a t e d

show abstract

Substance P in brain extracts

Cited by 48 publications

References 26 publications

An antistrychnine extract from horse intestine

An antistrychnine extract from horse intestine

Proceedings of the Physiological Society, 14 January 1967; Guy's Hospital Meeting: Communications

Tritium labelling of substance P and the corresponding N‐terminal 1‐7 fragment in the pro², pro⁴ positions

Contact Info

Product

Resources

About

Substance P in brain extracts

Cited by 48 publications

References 26 publications

An antistrychnine extract from horse intestine

An antistrychnine extract from horse intestine

Proceedings of the Physiological Society, 14 January 1967; Guy's Hospital Meeting: Communications

Tritium labelling of substance P and the corresponding N‐terminal 1‐7 fragment in the pro2, pro4 positions

Contact Info

Product

Resources

About

Tritium labelling of substance P and the corresponding N‐terminal 1‐7 fragment in the pro², pro⁴ positions