Subretinal Neovascularization in the Rat Induced by IRBP Synthetic Peptides

Animal models of choroidal and retinal neovascularization

“…Chan and colleagues reported and illustrated subretinal neovascularization in 10% of mice immunized with interphotoreceptor retinoid-binding protein (IRB) and developed experimental autoimmune uveoretinitis (EAU) (Chan et al, 1990). Sakamoto and co-workers immunized Lewis rats with a hind foot pad injection of an IRBP-derived synthetic peptide and intravenous Bordetella persusis (Sakamoto et al 1994). They found histologic evidence of inflammatory destruction of Bruchs membrane and subretinal neovascularization in 38% of the rats.…”

“…The inflammation decreased in intensity on day 18, but branches of retinal vessels were seen extending into the choroid. On day 45, there was no appreciable inflammation, but new vessels were present in 38% of the eyes (Sakamoto et al, 1994). …”

Superantigen Presentation by Human Retinal Pigment Epithelial Cells to T Cells is Dependent on CD2–CD58 and CD18–CD54 Molecule Interactions

“…Another disruption of the blood±retina barrier is seen at subretinal neovascularization which is a clinical feature of agerelated macular degeneration and may be caused by in¯ammation in the choroid (Kvanta et al, 1994). In a model, in which Lewis rats were immunized with interphotoreceptor retinoid binding protein (IRBP), it was shown by histological examination of the eyes that RPE cells, macrophages and lymphocytes accumulated in the subretinal space around the penetrating vessels as a result of in¯ammation in the choroid (Sakamoto et al, 1994). Altogether, these results show that the blood±retina barrier can be broken down and exposed to foreign antigen and in¯amma-tory cells.…”

Subfoveal Choroidal Neovascularization in Uveitis

“…In 1994, Sakamoto et al [6] induced subretinal neovas cularization in 38% of rats immunized with interphotoreccptor retinoid-binding protein peptide R4.…”