Subpopulations of Pig Blood E-Rosette-Forming Lymphocytes and Thymus-Dependent Null Cells: Separation by Nylon Wool Columns, Rosette Formation and Macrophage-Dependent Mitogen and Antigen Responsiveness

-Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD4 + CD8 low and CD4 − CD8 low T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. cluster of differentiation (CD) / monoclonal antibody / immune system / histocompatibility system / pig

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“…In swine, as well as in ruminants, there is a high proportion of null cells (≥30% in the blood of piglets [134,180] …”

Section: Markers Of Tcr-t Cells and 'Null Cells'mentioning

confidence: 99%

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

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“…Preliminary studies with non-pregnant gilts were undertaken to standardize and optimize cell preparations and LST in swine. T-cell reactivity was studied by mitogenic stimulation with Con A and PHA, both strong mitogens for T-lymphocytes in swine (Outterridge et al, 1980). AntiIg sera were used to measure B-cell reactivity (Symons et al, 1977).…”

Section: Lymphocyte Proliferative Responsementioning

confidence: 99%

The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow

Kortbeek-Jacobs

Kooten

Donk

et al. 1984

Veterinary Microbiology

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Kortbeek-Jacobs, J.M.C., van Kooten, P.J.S., van der Donk, J.A., van Dijk, J.E. and Rutten, V.P., 1984. The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow. Vet. Microbiol., 9: 287--299.Sows were immunized orally with live Escherichia coli according to various immunization schedules. Six pregnant gilts were used; 4 immunized at various intervals during the last month of gestation, 1 control immunized after parturition following suppression of lactation by weaning and 1 non-immunized control. The effect of oral vaccination on cell populations from lymphoid organs was studied. The in vitro proliferative responses of the cell populations to K88 antigen, anti-Ig sera and mitogens were used to demonstrate the distribution of sensitized lymphocytes over different lymphoid organs. The capacity of these cells to produce antigen-specific Ig was determined by in ovo translation of their mRNA. Oral administration of antigen resulted in the appearance of K88-positive cells in lymphoid organs. In lactating sows, sensitized cells preferentially occurred in the mammary lymph nodes, whereas after suppression of lactation such a distribution was not seen. A possible route of migration of sensitized lymphocytes is discussed in relation to the local immune response. The antibody isotype produced by sensitized lymphocytes seemed to depend on the immunization schedule. The most effective schedule was one starting early in gestation and comprising frequent administration of antigen. This caused an optimal distribution of sensitized lymphocytes capable of IgA production.

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Detection of primary direct and indirect human anti‐porcine T cell responses using a porcine dendritic cell population

et al. 1996

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The pathways of human anti-pig T cell xenorecognition have been investigated. Freshly isolated porcine alveolar lavage (AL) cells induced primary proliferative responses by human peripheral and cord blood mononuclear cells which were inhibited by anti-HLA-DR antibody (indirect xenorecognition). Following over-night culture, the AL cells acquired the capacity to stimulate proliferation by purified human T cells which was inhibited by anti-SLA-DR antibody (direct xenorecognition). The marked increase in immunogenicity in the porcine AL cells was accompanied by a phenotypic change consistent with dendritic cell maturation. Limiting dilution assays indicate that the total anti-pig T cell response, in particular that mediated by indirect xenorecognition, is stronger than comparable alloresponses.

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Subpopulations of Pig Blood E-Rosette-Forming Lymphocytes and Thymus-Dependent Null Cells: Separation by Nylon Wool Columns, Rosette Formation and Macrophage-Dependent Mitogen and Antigen Responsiveness

Cited by 14 publications

References 7 publications

Membrane markers of the immune cells in swine: an update

Membrane markers of the immune cells in swine: an update

The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow

Detection of primary direct and indirect human anti‐porcine T cell responses using a porcine dendritic cell population

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