Subpopulations in purified platelets adhering on glass

Donati, Alessia; Gupta, Swati; Reviakine, Ilya

doi:10.1116/1.4953866

Cited by 6 publications

(8 citation statements)

References 36 publications

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“…Platelet aggregation is linked to coagulation through initiation of platelet coagulant activity via the expression of negatively charged membrane phospholipids (phosphatidyl serine, PS), which catalyze clot formation. [ 72 ] Different platelet sub‐populations have been described based on the expression/presentation of PS, α IIb β 3 and CD62P. While an anti‐correlation between PS and α IIb β 3 has been described for platelets adhering on glass, also PS+/α IIb β 3 + populations have been reported.…”

Section: Resultsmentioning

confidence: 99%

“…[73] In view of these reports we hypothesize that the Pd glass and DLC surfaces induce the formation of a distinctly different platelet subpopulation when compared to Ti64 (and other "group 2" materials). However, as platelet pop ulations are considered snapshots of transient stages that are capable of interconversion, [72,73] further research in this direc tion is needed and foreseen for the here described materials and for engineered materials in general.…”

Section: Initiation Of Coagulation Cascade: Fibrinogen Adsorption Con...mentioning

confidence: 99%

See 1 more Smart Citation

Palladium‐Based Metallic Glass with High Thrombogenic Resistance for Blood‐Contacting Medical Devices

Cihova

Müller

Chandorkar

et al. 2021

Adv Funct Materials

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Advancements in the design of mechanical blood circulatory devices have greatly improved patient survival rates, but currently employed metals still provoke a thrombosis response upon contact with blood, with potential lifethreatening consequences. While coating strategies have been developed to address this limitation, they possess inherent drawbacks such as susceptibility to crack formation and delamination. Herein, an amorphous metal based on palladium (Pd) is scrutinized and reveals substantial thrombogenic resistance compared to a state-of-the-art titanium alloy. In vitro assessment with human whole blood shows that the Pd glass provokes reduced platelet activation (lower expression of CD62P, CD41/CD61) and greatly retarded fibrin formation, but pronounced platelet spreading therewith challenging the dogma that platelet spreading equals activation. Mechanistic analysis of the early biomaterial-blood interactions reveals that conformational changes of adhered fibrinogen, and modified α IIb β 3 integrin expression and distribution across adhered platelets, underlay the superior performance of Pd glass. The study is accompanied by structural and thermophysical bulk investigations and physicochemical surface characterization to link the materials properties with the observed blood response. The results reveal a remarkable potential of Pd-based glass as direct blood-contacting bulk material without the need for coating.

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Section: Resultsmentioning

confidence: 99%

Section: Initiation Of Coagulation Cascade: Fibrinogen Adsorption Con...mentioning

confidence: 99%

Palladium‐Based Metallic Glass with High Thrombogenic Resistance for Blood‐Contacting Medical Devices

Cihova

Müller

Chandorkar

et al. 2021

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Whether all platelets have the capacity to become procoagulant/COAT vs. aggregating platelet subpopulations or whether these subpopulations are predetermined requires further investigation. Studying platelet adhesion to glass some have argued that all platelets can form these subpopulations and that they simply reflect snapshots in time of a dynamic platelet activation process (69). However, multi-parameter flow cytometry studies of platelet responses to increasing concentrations of thrombin and CRP-XL showed that only a fraction of platelets can take on the procoagulant phenotype supporting the theory of distinct platelet subpopulations (70).…”

Section: Characterization Of Platelet Subpopulations Based On Differementioning

confidence: 99%

Advances in Platelet Subpopulation Research

Lesyk

Jurasz

2019

Front. Cardiovasc. Med.

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Although lacking a nucleus, platelets are increasingly recognized not only for their complexity, but also for their diversity. Some 50 years ago platelet subpopulations were characterized by size and density, and these characteristics were thought to reflect platelet aging. Since, our knowledge of platelet heterogeneity has grown to recognize that differences in platelet biochemistry and function exist. This includes the identification of vanguard and follower platelets, platelets with differing procoagulant ability including “COAT-platelets” which enhance procoagulant protein retention on their surface, and most recently, the identification of platelet subpopulations with a differential ability to generate and respond to nitric oxide. Hence, in this mini-review, we summarize the current knowledge of platelet subpopulation diversity focusing on their physical, biochemical, and functional heterogeneity. In addition, we review how platelet subpopulations may change between health and disease and how differences among platelets may influence response to anti-platelet therapy. Finally, we look forward and discuss some of the future directions and challenges for this growing field of platelet research.

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“…The current literature proposes that the development of platelet subpopulations may be based on a spectrum of activation, depending on the strength of stimulation and the cytoplasmic calcium concentration 57 , 58 . It has also been suggested that procoagulant and apoptotic platelets may be interrelated 24 , 25 , 34 .…”

Section: Discussionmentioning

confidence: 99%

Identification of platelet subpopulations in cryopreserved platelet components using multi-colour imaging flow cytometry

Johnson

Lei

Waters

et al. 2023

Sci Rep

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Cryopreservation of platelets, at − 80 °C with 5–6% DMSO, results in externalisation of phosphatidylserine and the formation of extracellular vesicles (EVs), which may mediate their procoagulant function. The phenotypic features of procoagulant platelets overlap with other platelet subpopulations. The aim of this study was to define the phenotype of in vitro generated platelet subpopulations, and subsequently identify the subpopulations present in cryopreserved components. Fresh platelet components (n = 6 in each group) were either unstimulated as a source of resting platelets; or stimulated with thrombin and collagen to generate a mixture of aggregatory and procoagulant platelets; calcium ionophore (A23187) to generate procoagulant platelets; or ABT-737 to generate apoptotic platelets. Platelet components (n = 6) were cryopreserved with DMSO, thawed and resuspended in a unit of thawed plasma. Multi-colour panels of fluorescent antibodies and dyes were used to identify the features of subpopulations by imaging flow cytometry. A combination of annexin-V (AnnV), CD42b, and either PAC1 or CD62P was able to distinguish the four subpopulations. Cryopreserved platelets contained procoagulant platelets (AnnV+/PAC1−/CD42b+/CD62P+) and a novel population (AnnV+/PAC1−/CD42b+/CD62P−) that did not align with the phenotype of aggregatory (AnnV−/PAC1+/CD42b+/CD62P+) or apoptotic (AnnV+/PAC1−/CD42b−/CD62P−) subpopulations. These data suggests that the enhanced haemostatic potential of cryopreserved platelets may be due to the cryo-induced development of procoagulant platelets, and that additional subpopulations may exist.

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Subpopulations in purified platelets adhering on glass

Cited by 6 publications

References 36 publications

Palladium‐Based Metallic Glass with High Thrombogenic Resistance for Blood‐Contacting Medical Devices

Palladium‐Based Metallic Glass with High Thrombogenic Resistance for Blood‐Contacting Medical Devices

Advances in Platelet Subpopulation Research

Identification of platelet subpopulations in cryopreserved platelet components using multi-colour imaging flow cytometry

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