Subnanometer optical coherence tomographic vibrography

Chang, Ernest W.; Kobler, James B.; Yun, Seok Hyun

doi:10.1364/ol.37.003678

Cited by 40 publications

(37 citation statements)

References 16 publications

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“…However, its widespread use in vibrometry has been limited for several reasons, including the vast quantity of data that must be acquired and processed very fast to make high frequency measurements, speed limitations inherent to spectrometerbased OCT, phase noise within the system, and difficulty in obtaining reliable and convincing anatomic images in vivo to localize the vibratory measurements (31). Here, we describe VOCTV, a technique which overcomes all these issues by combining swept-source technology, phase stabilization routines, and FPGA processing to provide high sampling and throughput rates with high sensitivity inside tissue.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea

Lee

Raphael

Park

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

177

145

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Sound is encoded within the auditory portion of the inner ear, the cochlea, after propagating down its length as a traveling wave. For over half a century, vibratory measurements to study cochlear traveling waves have been made using invasive approaches such as laser Doppler vibrometry. Although these studies have provided critical information regarding the nonlinear processes within the living cochlea that increase the amplitude of vibration and sharpen frequency tuning, the data have typically been limited to point measurements of basilar membrane vibration. In addition, opening the cochlea may alter its function and affect the findings. Here we describe volumetric optical coherence tomography vibrometry, a technique that overcomes these limitations by providing depthresolved displacement measurements at 200 kHz inside a 3D volume of tissue with picometer sensitivity. We studied the mouse cochlea by imaging noninvasively through the surrounding bone to measure sound-induced vibrations of the sensory structures in vivo, and report, to our knowledge, the first measures of tectorial membrane vibration within the unopened cochlea. We found that the tectorial membrane sustains traveling wave propagation. Compared with basilar membrane traveling waves, tectorial membrane traveling waves have larger dynamic ranges, sharper frequency tuning, and apically shifted positions of peak vibration. These findings explain discrepancies between previously published basilar membrane vibration and auditory nerve single unit data. Because the tectorial membrane directly overlies the inner hair cell stereociliary bundles, these data provide the most accurate characterization of the stimulus shaping the afferent auditory response available to date.hearing | cochlea | mechanics | vibrometry | auditory system

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Section: Discussionmentioning

confidence: 99%

Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea

Lee

Raphael

Park

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

177

145

View full text Add to dashboard Cite

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“…A home-built phase-sensitive OCT system [19] was used, which employed a polygonscanner swept laser in the spectral range from 1.22 to 1.34 µm with a typical sweep rate of 48 kHz and an optical power of 15 mW on the sample. The reflectivity sensitivity of the OCT system was 105 dB.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…The spatial profile of corneal vibration was determined by using the principle of vibrography previously described [19,20]. Briefly, at a constant sound frequency the optical beam was scanned along a line (~10 mm) or over an area.…”

Section: Experimental Methodsmentioning

confidence: 99%

Observation of sound-induced corneal vibrational modes by optical coherence tomography

Akca

Chang

Kling

et al. 2015

Biomed. Opt. Express

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Abstract:The mechanical stability of the cornea is critical for maintaining its normal shape and refractive function. Here, we report an observation of the mechanical resonance modes of the cornea excited by sound waves and detected by using phase-sensitive optical coherence tomography. The cornea in bovine eye globes exhibited three resonance modes in a frequency range of 50-400 Hz. The vibration amplitude of the fundamental mode at 80-120 Hz was ~8 µm at a sound pressure level of 100 dB (2 Pa). Vibrography allows the visualization of the radially symmetric profiles of the resonance modes. A dynamic finite-element analysis supports our observation. ©2015 Optical Society of America

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“…The choice of m should at the minimum satisfy the Nyquist criteria while m > 6 is desirable for optimal results. 13 However, a higher temporal oversampling factor may be needed if the displacements are large to prevent any phase-wrapping problems and for more accurate phase lag estimates. In Fig.…”

Section: Influence Of Scanning Parameters On Mm-oct Signalmentioning

confidence: 99%

“…MM excitation in the form of pulsed waveforms can be used to minimize heat generation in the coil and the sample, and increase the operating range. 11 However, leveraging the capability of lock-in detection using a sinusoidal excitation of a known frequency allows for much better noise rejection (hence, higher SNR) 12,13 and can also enable more quantitative evaluation based on the phase and amplitude response of the sample under harmonic excitation. 14 In this paper, we first discuss the operating factors that determine the MM-OCT data acquisition speed and then demonstrate an order-of-magnitude improvement in the imaging speed for the acquisition of a 3-D MM-OCT dataset using a modified scanning scheme.…”

Section: Introductionmentioning

confidence: 99%

Volumetric full-range magnetomotive optical coherence tomography

et al. 2014

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Abstract. Magnetomotive optical coherence tomography (MM-OCT) can be utilized to spatially localize the presence of magnetic particles within tissues or organs. These magnetic particle-containing regions are detected by using the capability of OCT to measure small-scale displacements induced by the activation of an external electromagnet coil typically driven by a harmonic excitation signal. The constraints imposed by the scanning schemes employed and tissue viscoelastic properties limit the speed at which conventional MM-OCT data can be acquired. Realizing that electromagnet coils can be designed to exert MM force on relatively large tissue volumes (comparable or larger than typical OCT imaging fields of view), we show that an order-of-magnitude improvement in three-dimensional (3-D) MM-OCT imaging speed can be achieved by rapid acquisition of a volumetric scan during the activation of the coil. Furthermore, we show volumetric (3-D) MM-OCT imaging over a large imaging depth range by combining this volumetric scan scheme with full-range OCT. Results with tissue equivalent phantoms and a biological tissue are shown to demonstrate this technique.

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Subnanometer optical coherence tomographic vibrography

Cited by 40 publications

References 16 publications

Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea

Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea

Observation of sound-induced corneal vibrational modes by optical coherence tomography

Volumetric full-range magnetomotive optical coherence tomography

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