Submicroscopic Organization of the Nucleus during Spermiogenesis in the Grasshopper

Dass, C. M. S.; Ris, Hans

doi:10.1083/jcb.4.1.129

Cited by 50 publications

(12 citation statements)

References 15 publications

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“…Similar examples of nuclear condensation have been described in various animal species, both invertebrate (Dass and Ris, 1957;Gibbons and Bradfield, 1957;Rebhun, 1957;Yasuzumi and Ishida, 1957;Gall and Bjork, 1958) and vertebrate Fawcett, 1955, 1956;Nagano, 1962). The detectable structural changes which the nuclear material goes through appear to vary considerably from one species to another.…”

Section: Discussionmentioning

confidence: 65%

An Ultrastructural Study of Plant Spermatogenesis

Turner

1968

The Journal of Cell Biology

118

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Spermatogenesis in the charophyte Nitella has been followed in antheridia prepared for light and electron microscopy. The antheridial filament cells contain paired centrioles which are similar in structure and behavior to the centrioles of animal cells. In the early spermatid, the centrioles undergo an initial elongation at their distal ends and become joined by a spindle-shaped fibrous connection. At the same time, their proximal ends are closely associated with the development of a layer of juxtaposed microtubules which will form the microtubular sheath. The architectural arrangement of these microtubules suggests that they constitute a cytoskeletal system, forming a framework along which the mitochondria and plastids become aligned and along which the nucleus undergoes extensive elongation and differentiation. The microtubular sheath persists in the mature sperm. During mid-spermatid stages, the centrioles give rise to the flagella and concomitantly undergo differentiation to become the basal bodies. The Golgi apparatus goes through a period of intensive activity during mid-spermatid stages, then decreases in organization until it can no longer be detected in the late spermatid. An attempt is made to compare similarities between plant and animal spermiogenesis.

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Section: Discussionmentioning

confidence: 65%

An Ultrastructural Study of Plant Spermatogenesis

Turner

1968

The Journal of Cell Biology

118

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“…Condensation ended abruptly at a large concavity comprising perhaps one-third of the total perimeter, and nuclear material (presumably chromatin) appeared to be withdrawn from the nuclear membrane in (2,6), was juxtaposed against the convex surface of the head (Figs. 3,5,7,9,10). As differentiation proceeded, the nucleus elongated and chromatin was condensed to a greater and greater extent until the entire head was uniformly electron opaque; the concavity containing the microtubular bundle disappeared (Figs.…”

Section: Electron Microscopymentioning

confidence: 99%

SPERMIOGENESIS IN WILD TYPE AND IN A MALE STERILITY MUTANT OF DROSOPHILA MELANOGASTER

Shoup

1967

The Journal of Cell Biology

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Spermiogenesis in the translocation heterozygote T (1; 2H) 25(20) y l 25/FM6 has been studied with the electron microscope and compared with that in wild type males. It appears that the genetic lesion in the male sterility mutant is associated primarily with a failure in differentiation of the head. In wild type flies, the spermatid nucleus assumes a conchoidal shape; chromatin accumulates along the convex surface. Adjacent to the concave surface a large bundle of microtubules runs parallel to the long axis of the spermatid. A single row of microtubules is juxtaposed against the convex surface of the head. As differentiation proceeds, the nucleus elongates, chromatin condenses, and the nucleus is compacted to a final diameter of about 0.3 #. In the sterile mutant the spermatid nucleus has an irregular or wedge-shaped profile and no concavity is formed, nor is the bundle of microtubules observed. The row of microtubules, however, is usually present around the periphery. The change from lysine-rich to arginine-rich histone in mature wild type sperm does not occur in the sterile male. The substructure of the axial filament and mitochondrial derivatives, however, are similar to those in wild type.

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“…Bloch and Brack (1964) reported that, in the grasshopper Chortophaga, arginine-rich histone is synthesized in the cytoplasm fairly late in spermiogenesis and subsequently migrates into the nucleus . Dass and Ris (1958) felt that there was a correlation between the shift in nuclear basic proteins and a change in the appearance of chromatin fibers . They found that the chromatin of young spermatids of the grasshopper Chorthippus was made up of 200-250 A fibers, each composed of two 100 A subunits .…”

Section: Nuclear Condensationmentioning

confidence: 99%