Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Abstract

Basidiomycete fungi of the Polyporus genus are a source of secondary metabolites which are of medicinal interest as antibacterial compounds. As these substances are produced in a small amount by the fungi, the study of the cultivation conditions in vitro that could possibly optimize their production seems of major importance. The effects of glucose and lactose, pH and agitation on biomass concentration and on the specific growth rate caused by the basidiomycete Polyporus tricholoma were investigated. The initial pH (4.5, 6.5 and 8.5) was autoregulated at pH 5.5, and the agitation increased the mycelial growth and the specific growth rate. The high concentration of carbon sources (4%) increased biomass production. The lactose concentration and the absence of agitation were determinant in the production of antibacterial metabolites. The characterization of the antibacterial substance by GC-MS indicated a major compound, isodrimenediol, produced by the fungus Polyporus tricholoma with activity against Staphylococcus aureus.

Expression of manganese peroxidase by Lentinula edodes and Lentinula boryana in solid state and submerged system fermentation

“…V.M.C.S Santos (unpublished data) reports that some species of basidiomycetes have the characteristic of self-regulating pH, tending to stabilize its value in the optimum pH for growth, regardless the initial pH value. This fact was also observed by Vieira et al (2008) in a study with Polyporus tricoloma for antibiotic production. The initial ph value does not seem to influence the lengthening of hyphae in Macrocybe titans as well.…”

Optimization of exopolysaccharides production from a novel strain of Ganoderma lucidum CAU5501 in submerged culture

“…Over the past decades there has been an increasing attention to the production of polysaccharides from mushrooms due to their various physiological activities (14, 17). Ganoderma lucidum , a medicinal fungus called "Lingzhi" in Chinese and "Reishi" in Japanese, has been widely used as a remedy for promotion of health and longevity in the Far East, especially in China, Japan and Korea for centuries.…”

Modification of c and n sources for enhanced production of cyclosporin 'a' by Aspergillus Terreus

“…Although cellulose is a complex carbohydrate (polysaccharide), but A. terreus is known to produce extracellular endo-β-1, 4-glucanase, exo-β-1, 4-glucanase with high levels of β-glucosidase and has the ability to utilize cellulose as carbon source. Previous studies also show increase in fungal biomass with increase of carbon sources (29).…”