Submerged cultivation of selected macro-fungi to produce mycelia rich in β-glucans and other bioactive compounds, valorizing side streams of the food industry

Pilafidis, Sotirios; Tsouko, Erminta; Sougleri, Glykeria; Diamantopoulou, Panagiota; Gkatzionis, Konstantinos; Ioannou, Zacharias; Sarris, Dimitris

doi:10.1016/j.crcon.2023.09.002

Cited by 12 publications

(6 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…that in general present excellent growth on glycerol [26,28,50]. A noticeable exception to this rule is the Zygomycete Thamnidium elegans as well as some strains of macrofungi (i.e., Ganoderma lingzhi, Grifola frondosa) that presented, indeed, very interesting microbial growth on media composed of glycerol, almost similar to the one obtained on glucose [49,51].…”

Section: Discussionmentioning

confidence: 86%

Poly-Unsaturated Fatty Acids (PUFAs) from Cunninghamella elegans Grown on Glycerol Induce Cell Death and Increase Intracellular Reactive Oxygen Species

Kalampounias,

Gardeli,

Alexis

et al. 2024

JoF

View full text Add to dashboard Cite

Cunninghamella elegans NRRL-1393 is an oleaginous fungus able to synthesize and accumulate unsaturated fatty acids, amongst which the bioactive gamma-linolenic acid (GLA) has potential anti-cancer activities. C. elegans was cultured in shake-flask nitrogen-limited media with either glycerol or glucose (both at ≈60 g/L) employed as the sole substrate. The assimilation rate of both substrates was similar, as the total biomass production reached 13.0–13.5 g/L, c. 350 h after inoculation (for both instances, c. 27–29 g/L of substrate were consumed). Lipid production was slightly higher on glycerol-based media, compared to the growth on glucose (≈8.4 g/L vs. ≈7.0 g/L). Lipids from C. elegans grown on glycerol, containing c. 9.5% w/w of GLA, were transformed into fatty acid lithium salts (FALS), and their effects were assessed on both human normal and cancerous cell lines. The FALS exhibited cytotoxic effects within a 48 h interval with an IC50 of about 60 μg/mL. Additionally, a suppression of migration was shown, as a significant elevation of oxidative stress levels, and the induction of cell death. Elementary differences between normal and cancer cells were not shown, indicating a generic mode of action; however, oxidative stress level augmentation may increase susceptibility to anticancer drugs, improving chemotherapy effectiveness.

show abstract

Section: Discussionmentioning

confidence: 86%

Poly-Unsaturated Fatty Acids (PUFAs) from Cunninghamella elegans Grown on Glycerol Induce Cell Death and Increase Intracellular Reactive Oxygen Species

Kalampounias,

Gardeli,

Alexis

et al. 2024

JoF

View full text Add to dashboard Cite

show abstract

“…The vibrations at 1666-1621, 1560-1542 and 1450-1440 cm −1 have been attributed to the presence of proteins [48,52,53] and the C=C and C=O stretching of amino acids, carboxylates, phenols and chitin/chitosan [49,54]. The absorption at 1163-1152, 1086-1082, 1043-1039 and 991-989 cm −1 have been attributes to carbohydrates [51,52,55], while the absorption at 926-905 and 893-891 cm −1 have been attributed to βand αglycosidic bonds, mainly in αand β-glucans [49]. The observed shifts at 1560-1542 (150 BOS, 150 WS), 1163-1139 and 926-905 cm −1 (144WS) are most likely due to intermolecular interactions.…”

Section: Amino Acid Compositionmentioning

confidence: 99%

“…Several researchers have studied the components of P. ostreatus. Polysaccharides and, in particular, α-and β-glucans [48][49][50]54,56], proteins [18,49], phospholipids [56], chitin/chitosan [54] and amino acids [57] have been identified. Thus, the spectroscopic findings are in agree with the literature.…”

Section: Amino Acid Compositionmentioning

confidence: 99%

See 1 more Smart Citation

The Influence of Substrate and Strain on Protein Quality of Pleurotus ostreatus

Gardeli,

Mela,

Dedousi

et al. 2024

Applied Sciences

Self Cite

View full text Add to dashboard Cite

Background: The effect of substrate and strain on the nutritional and functional properties of mushroom flours and protein concentrates (PCs) has not thoroughly been investigated. Methods: The proteins of P. ostreatus flours (strains AMRL144 and 150) were isolated following alkaline extraction (pH 12) and isoelectric precipitation (pH 4) as it was determined by the solubility curves. The protein quality of the flours and PCs were evaluated by determining the protein solubility index (PSI) and their functional properties, such as water (WAC) and oil absorption capacity (OAC), foam capacity (FC), foam stability (FS) and emulsion stability (ES). The amino acid (AA) composition of the flours was determined by reversed-phase liquid chromatography after protein hydrolysis and o-phthalaldehyde derivatization. Results: The PSIs of the flours and PCs of P. ostreatus were comparable to those of soy protein flours and protein concentrates. The highest AA concentration was found for both strains when cultivated on the barley and oat straw (BOS) substrate, showing a similar trend as the protein content. A principal component analysis (PCA) indicated an impact of the strain on the functional properties. Conclusions: Both strains can produce high quality proteins especially when cultivated on wheat straw (WS). The FS was positively correlated with the P. ostreatus strain AMRL150 whereas the FC was positively correlated with AMRL144.

show abstract

“…Previous studies have indicated that apart from agitation, the type of substrate and carbon source can entail modifications on the protein content. For instance, Pilafidis et al used wine distillery effluents and brewer's spent grain as substrates for submerged cultivation of several macro-fungi, including T. versicolor [37]. The authors reported that brewer's spent grain influenced the protein content, whereas when wine distillery effluents were evaluated, the protein content was not affected [37].…”

Section: Evaluation Of Crude Intracellular (Ips) and Extracellular (E...mentioning

confidence: 99%

Valorization of Grape Pomace for Trametes versicolor Mycelial Mass and Polysaccharides Production

Kachrimanidou,

Alexandri,

Papapostolou

et al. 2023

Sustainability

View full text Add to dashboard Cite

Polysaccharides and protein–polysaccharide complexes produced from the fungal strain Trametes versicolor have demonstrated bioactive properties that depend on the substrate, the fermentation conditions and also the fungal strain. Likewise, the submerged and controlled fermentation of medicinal mushrooms elicits numerous advantages over traditional processes to produce mycelia and added-value products, along with the exploitation of biodiversity. This study evaluated the growth profile of an indigenous T. versicolor isolate using commercial nutrients that were subsequently replaced with renewable resources, specifically, grape pomace extract (GPE), under static and shaking conditions. The effect of elicitor addition was also assessed using GPE. The process productivity was significantly improved, yielding 21 g/L of biomass. Agitation proved beneficial for all examined cases regarding biomass productivity and substrate consumption rates. The chemical and antioxidant profile of crude intracellular and extracellular polysaccharides was determined, whereby intracellular extracts indicated >50% antioxidant activity. FTIR analysis validated the preliminary chemical characterization of the extracts, whereas the amino acid profile of IPS extracts was also included. Evidently, our study elaborates on the development of a bioconversion concept to valorize wine-making side-streams to formulate added-value products with potential bioactive attributes.

show abstract

Submerged cultivation of selected macro-fungi to produce mycelia rich in β-glucans and other bioactive compounds, valorizing side streams of the food industry

Cited by 12 publications

References 112 publications

Poly-Unsaturated Fatty Acids (PUFAs) from Cunninghamella elegans Grown on Glycerol Induce Cell Death and Increase Intracellular Reactive Oxygen Species

Poly-Unsaturated Fatty Acids (PUFAs) from Cunninghamella elegans Grown on Glycerol Induce Cell Death and Increase Intracellular Reactive Oxygen Species

The Influence of Substrate and Strain on Protein Quality of Pleurotus ostreatus

Valorization of Grape Pomace for Trametes versicolor Mycelial Mass and Polysaccharides Production

Contact Info

Product

Resources

About