Submaxillary gland recovery from obstruction. I. Overall changes and electron microscopic alterations of granular duct cells

Tamarin, Arnold

doi:10.1016/s0022-5320(71)80072-2

Cited by 103 publications

(80 citation statements)

References 9 publications

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“…Clinically, obstructive functional disorder of the salivary gland is a pathology that develops over a long period of time, due to increases in salivary calculi or other substances. To date, reports on submandibular gland main excretory duct ligation models have mostly been short ligation periods of around 1 week [19][20][21][22][23] , and the longest have been approximately 1 month 8,11) . Thus, there is a need for studies investigating the tendency toward atrophy and residual regeneration function in salivary glands in conditions closer to actual clinical pathology.…”

Section: Discussionmentioning

confidence: 99%

“…Histologically, atrophy and disappearance of acinar cells, and gradual increases in duct-like structures and fibrous connective tissue were observed. Changes in salivary gland tissue after short-term ligation that have been reported include enlargement of duct lumens, atrophy and disappearance of acinar cells above the ligation, increases in ductlike structures and proliferation of fibrous connective tissue, degranulation and vacuolar degeneration of granular duct cells and acinar cells, and disappearance of basal striations from the striated duct [8][9][10][11][12][13][14][15][16][17][18] . This was similar to findings in the long-term ligation.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in salivary gland tissue that have been found as a result of duct ligation include atrophy and disappearance of acinar cells above the ligation, an increase in duct-like structures, and an increase in fibrous connective tissue [8][9][10][11][12][13][14][15][16][17][18] . There are also reports noting residual salivary gland function, restorative function, and regenerative ability in atrophied salivary glands [19][20][21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Murayama

Kawakami

Tanaka

2017

J. Hard Tissue Biology.

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To examine the pathology of salivary glands that have undergone atrophy or hypofunction due to old age or disease, the duct ligation model is used. This model has been used in studies on the course of glandular parenchyma atrophy and the potential for repair and regeneration. However, the period of ligation was short in most of these studies, and none have examined long-term progress. Therefore, we investigated long-term ligation of the submandibular gland in mice and examined the chronic changes in atrophied salivary glands. The ligation periods were 1, 2 and 3 months, and atrophied salivary glands were resected after each period. Resected glands were examined by histology, immunohistochemistry, RT-PCR, and transmission electron microscopy. Histologically, disappearance of acinar cells and increases in duct-like structures were observed over time in atrophied salivary glands, resulting from long-term submandibular gland main excretory duct ligation. Acinar cell markers (-amylase, aquaporin 5) showed marked weakness in expression after ligation, but expression was still observed after 3 months of ligation. Stem cell markers (c-kit, Sca-1) showed greater expression at 1 month of ligation, compared with controls, but expression subsequently decreased with time. Expression of the precursor cell marker cytokeratin 5 was retained throughout long-term ligation. Atrophied salivary gland tissue resulting from long-term ligation showed increases in specific duct-like structures over time, and the expression of stem cell markers and progenitor cell markers in the area of these structures suggests that the repair capability remained intact.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Murayama

Kawakami

Tanaka

2017

J. Hard Tissue Biology.

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“…), the excretory duct of the parotid gland was carefully isolated from con nective tissue and nerve under a surgical microscope. The duct was ligated, and then the ligature was removed according to the procedure of Tamarin (8). On day 2 and 7 after the ligation, the ligature was removed; and after 7, 14 and 21 days, respectively, the rats were killed.…”

Section: Methodsmentioning

confidence: 99%

Parotid Gland Recovery from an Obstruction—Changes of Amylase Release from the Tissue

Murata

1986

Japanese Journal of Pharmacology

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Abstract-The recovery of the parotid gland in the rat was studied by isoproterenol induced amylase release from the parotid tissue in vitro after the removal of duct ligation following 2 and 7 days of obstruction.The percentage of amylase release did not change, but the activity of amylase in the medium (the released amylase activity) increased gradually after the removal of ligation.When the duct was ligated for 2 days, a complete recovery of the released amylase activity was seen 21 days after the removal of ligature.However, in the case of 7-day-ligation, the recovery was about 70 percent after the same period. An obstruction of a shorter duration produced a more rapid recovery of amylase release.Amylase activity in the parotid tissue increased gradually with time after the removal of ligature, and the recovery rates were very similar to that of the released amylase activity. The present results suggest that the recovery of released amylase activity after the removal of ligature is due to the increase of amylase content in the parotid tissue.

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“…After removal of the ligation, both the submandibular and parotid glands have restored acinar structures, which supports some inherent but limited gland regeneration. [4][5][6] No salivary gland stem cell has been definitively identified as contributing to gland regeneration; however, several duct cell subtypes have been characterized as progenitor cells. [7][8][9][10][11][12] Furthermore, although the direct injection of progenitor cell populations, namely c-Kit + salivary progenitor cells 10,13 or mesenchymal stem cells (MSCs), 14 into irradiated submandibular glands (SMGs) showed some functional improvement, restoration of saliva secretion was incomplete, and highly variable.…”

mentioning

confidence: 99%

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

Shubin

Felong

Graunke

et al. 2015

Tissue Engineering Part A

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More than 40,000 patients are diagnosed with head and neck cancers annually in the United States with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced xerostomia, but they suffer from insufficient and inconsistent gland regeneration and accompanying secretory function. To aid in the development of regenerative therapies, poly(ethylene glycol) hydrogels were investigated for the encapsulation of primary submandibular gland (SMG) cells for tissue engineering applications. Different methods of hydrogel formation and cell preparation were examined to identify cytocompatible encapsulation conditions for SMG cells. Cell viability was much higher after thiol-ene polymerizations compared with conventional methacrylate polymerizations due to reduced membrane peroxidation and intracellular reactive oxygen species formation. In addition, the formation of multicellular microspheres before encapsulation maximized cell-cell contacts and increased viability of SMG cells over 14-day culture periods. Thiol-ene hydrogel-encapsulated microspheres also promoted SMG proliferation. Lineage tracing was employed to determine the cellular composition of hydrogel-encapsulated microspheres using markers for acinar (Mist1) and duct (Keratin5) cells. Our findings indicate that both acinar and duct cell phenotypes are present throughout the 14 day culture period. However, the acinar:duct cell ratios are reduced over time, likely due to duct cell proliferation. Altogether, permissive encapsulation methods for primary SMG cells have been identified that promote cell viability, proliferation, and maintenance of differentiated salivary gland cell phenotypes, which allows for translation of this approach for salivary gland tissue engineering applications.

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Submaxillary gland recovery from obstruction. I. Overall changes and electron microscopic alterations of granular duct cells

Cited by 103 publications

References 9 publications

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Chronic Changes in the Atrophied Submandibular Gland after Long-term Ligation of the Main Excretory Duct in Mice

Parotid Gland Recovery from an Obstruction—Changes of Amylase Release from the Tissue

Development of Poly(Ethylene Glycol) Hydrogels for Salivary Gland Tissue Engineering Applications

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