Sublytic C5b-9 Complexes Induce Apoptosis of Glomerular Mesangial Cells in Rats with Thy-1 Nephritis through Role of Interferon Regulatory Factor-1-dependent Caspase 8 Activation

Li, Lisha; Qiu, Wen; Wang, Hui; Li, Yan; Zhou, Jianbo; Mao, Xia; Shan, Kai; Pang, Rongrong; Zhou, Ying; Zhao, Dan; Wang, Yingwei

doi:10.1074/jbc.m111.319566

Cited by 29 publications

(60 citation statements)

References 50 publications

(64 reference statements)

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“…Our study showed that plasma concentration of C5b-9 significantly lower in CAL. Maybe most C5b-9 was primarily deposits in the glomerulus and lead lower blood concentration (26). There may be an association with other increased complement regulatory proteins (CD35, CR1, CR2, and Crry) in vivo, which regulate immune status and inhibit complex formation, with C5b-9 in dynamic balance, but the specific biological mechanism needs further study.…”

Section: Discussionmentioning

confidence: 99%

Plasma MASP-1 concentration and its relationship to recovery from coronary artery lesion in children with Kawasaki disease

Song

Zou

et al. 2015

Pediatr Res

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Background: This study investigated prognostic factors for early recovery of coronary artery lesion (CAL) in children with Kawasaki disease (KD). Methods: Patients hospitalized for KD were enrolled less than 2 wk from the onset of illness and divided into two groups: KD with CAL and KD without CAL. The CAL group was further divided into two subgroups according to the degree of CAL: mild (n = 31) and moderate/severe (n = 6) and further divided into two subgroups according to the age: younger than 1 y (n = 9) and older than 1 y (n = 28). Lectin pathway-related factors MASP-1, CD59, and C5b-9 were measured, along with C-reactive protein, white blood cell counts, erythrocyte sedimentation rate, and platelet count. Patients were followed up for 3 mo. Correlation between the measured factors and the length of time of recovery from CAL was analyzed. results: Plasma concentrations of MASP-1 in the CAL group were significantly lower than those without CAL. MASP-1 and gender positively correlated with the recovery time of CAL. There was no difference in MASP-1 between mild and moderate/severe CAL. At 3-mo follow-up, there was a positive correlation between plasma MASP-1 concentration and recovery time of the patients with CAL older than 1 y. conclusion: Plasma MASP-1 concentration at the early stage of KD is predictive of length of time of recovery from CAL. k awasaki disease (KD), also known as mucocutaneous lymph node syndrome, is an acute nonspecific systemic vascular inflammatory syndrome occurring mainly in children under 5 y of age. A considerable volume of research has shown that the pathological process of KD is characterized by immune activation and immune vasculitis of the vascular endothelial cells (1). KD mostly affects the small and mediumsized arteries, especially the coronary artery (2), resulting in coronary artery lesion (CAL), such as dilation, aneurysm formation, or advanced stenosis, and it is the leading cause of death in children with KD (3). It has been reported that 15-25% of untreated KD patients may develop CAL (4). Although treatment with intravenous immunoglobulin within 10 d of onset of fever reduces the incidence of CAL, 5-8% of patients still develop it. The degree of CAL varies considerably between individuals, ranging from mild to moderate ectasia of the coronary artery to giant aneurysm formation. The recovery time of CAL also differs between patients. There is a very little information about the length of time for which different degrees of CAL persists. Little is known on the relationship between the complement system and the recovery time of CAL resulting from KD. Our previous study indicated that the lectin pathway of the complement system is activated in KD. CAL is associated with endothelial cell dysfunction induced by T-cell activation (5). Endothelial cells are a source of complement-related factors and complement regulatory factors (6), and MASP-1 activates endothelial cells directly, which then activates the complement lectin pathway (7). Endothelial cell dysfunction (8) and com...

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Section: Discussionmentioning

confidence: 99%

Plasma MASP-1 concentration and its relationship to recovery from coronary artery lesion in children with Kawasaki disease

Song

Zou

et al. 2015

Pediatr Res

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“…The mechanism of anti-Thy1 glomerulonephritis as an experimental model for mesangial proliferative glomerulonephritis was reported by some investigators [30,31] . Administration of anti-Thy1 to rats leads to proliferative glomerulonephritis, which is characterized by complement dependent injury of mesangial cells, followed by mesangial proliferation.…”

Section: Discussionmentioning

confidence: 81%

“…Primer sequences for SRY gene (forward, 5'-CAT CGA AGG GTT AAA GTG CCA-3', reverse 5'-ATA GTG TGT AG-GTT GTT GTC C-3') were obtained from published sequences by Bruckner et al and amplified a product of 104 bp [15] . The PCR conditions were as follow: incubation at 94 °C for 4 min; 35 cycles of incubation at 94 °C for 50 seconds, 60 °C for 30 seconds, and 72 °C for 1 min; with a final incubation at 72 °C for 10 min. PCR products were separated using 2% agarose gel electrophoresis and stained with ethidium bromide.…”

Section: Pcr Detection Of Male-derived Mscsmentioning

confidence: 99%

Effect of mesenchymal stem cells on anti-Thy1,1 induced kidney injury in albino rats

Sakr

Rashed

Zarouk

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

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“…Furthermore, sublytic C5b-9 deposition induces apoptosis by up-regulating the interferon regulatory factor-1 caspase 8/caspase 3 pathways, an event that leads ultimately to endothelial cell detachment and subsequent activation of the indirect clotting pathway via exposure of type IV collagen (20). Eculizumab would effectively block these MAC-mediated procoagulant events.…”

Section: Discussionmentioning

confidence: 99%

“…In renal transplant recipients, it has been used for the treatment of a variety of complement/ antibody-mediated microangiopathy syndromes including aHUS (24,25) and antibody-mediated rejection (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26) and in single case studies of patients with CAPS (13) and APS (27).…”

Section: Discussionmentioning

confidence: 99%

Eculizumab Prevents Recurrent Antiphospholipid Antibody Syndrome and Enables Successful Renal Transplantation

Lonze

Zachary

Magro

et al. 2014

American Journal of Transplantation

140

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Sublytic C5b-9 Complexes Induce Apoptosis of Glomerular Mesangial Cells in Rats with Thy-1 Nephritis through Role of Interferon Regulatory Factor-1-dependent Caspase 8 Activation

Cited by 29 publications

References 50 publications

Plasma MASP-1 concentration and its relationship to recovery from coronary artery lesion in children with Kawasaki disease

Plasma MASP-1 concentration and its relationship to recovery from coronary artery lesion in children with Kawasaki disease

Effect of mesenchymal stem cells on anti-Thy1,1 induced kidney injury in albino rats

Eculizumab Prevents Recurrent Antiphospholipid Antibody Syndrome and Enables Successful Renal Transplantation

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