Sublocalization of an invasion-inducing locus and other genes on human chromosome 7

Abstract

By somatic cell fusion studies between noninvasive mouse T-lymphoma cells and invasive human activated normal T-cells we have previously shown that the genetic information responsible for the induction of invasive and metastatic potential in interspecies T-cell hybrids is located on human chromosome 7. Apparently, genes derived from normal activated T-cells are dominantly expressed in the hybrids and control the invasive and, as a consequence, metastatic potential of these T-lymphoma cells. To sublocalize the invasion-inducing locus on chromosome 7 we have generated hybrids that harbor only specific regions of human chromosome 7 with or without a small fragment of human chromosome 21. Analysis of these hybrids revealed that the invasion-inducing locus maps to 7p12→cen. The human DNA complement of the hybrids was confirmed by Southern blot analysis using a large panel of chromosome 7-specific DNA probes. Several of these genes could be further sublocalized. These included: ARAF2 to 7p12→cen, D7S21 to 7pter→p12, ACTB to 7p15→p12, EGFR to 7p12, MDH2 to 7cen→q22, and PDGFA to 7pter→p15.

Detailed marker chromosome analysis in cell line U-BLC1, established from transitional-cell carcinoma of the bladder

“…The map of the latter amplification unit includes the gene of the epidermalgrowth-factor receptor, EGFR (Habets et al, 1992). FISH with an EGFR cDNA probe labels the proximal three quarters of the HSR of marker 1 (results not shown), which is in line with the hybridization results obtained with the WCP 7 probe (Fig.…”

“…The putative target of the amplification of 7p11-p12 was the gene for the epidermal-growth-factor receptor, EGFR (Neal et al, 1990;Habets et al, 1992). FISH analysis with an EGFR-specific cDNA probe on tumor chromosomes (results not shown), semi-quantitative PCR and Northern blot analysis (Fig.…”

Comparative genomic hybridization analysis of primary colorectal carcinomas and their synchronous metastases

“…Also, gain of chromosome arm 7p, known to harbor epidermal growth factor receptor (EGFR) and T-cell tumor invasion and metastasis-1 (TTIM1) genes (Kondo and Shimizu, 1983), was frequently found (8/12) in primary tumors and/or their metastases. Somatic cell fusion studies (Collard et al, 1987;Habets et al, 1992) have shown that the invasive capabilities of hybrid cells depended on the presence of chromosome fragment 7p12-cen.…”

A strong intronic enhancer element of the EGFR gene is preferentially active in high EGFR expressing breast cancer cells

“…The EGFR gene is 110 kb in size, has 26 exons and is located on chromosome 7p12 [Habets et al, 1992]. The ®rst intron, which is the focus of our studies, is 18 kb in size.…”