Sublethal injury and recovery of Escherichia coli O157:H7 and K-12 after exposure to lactic acid

Shi, Huixian; Chen, Zhuozhu; Dong, Chen; Kan, Jianquan

doi:10.1016/j.foodcont.2017.06.017

Cited by 25 publications

(24 citation statements)

References 39 publications

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“…As reported in our previous study, sublethally injured E. coli cells widely persisted under different levels of lactic acid stress (Shi et al ). Generic E. coli has been regarded as an indicator organism for possible contamination of a variety of food products and fabrication facility in food plants (Brown et al ; Yang et al ).…”

Section: Introductionsupporting

confidence: 74%

“…No significant difference of the recovery ratios between injured cells in minA with 1·0 mmol l −1 zinc and in minA with 3·0 mmol l −1 CAT was found ( P ˃ 0·05). In our previous study, sodium pyruvate with higher concentration (0·05%/4·5 mmol l −1 ) also significantly increased the recovery ratio ( P < 0·05) (Shi et al ). Recently, it was reported that pyruvate was taken up as a carbon source for the resuscitation of the other dormant state‐viable but nonculturable E. coli K‐12 cells (Vilhena et al ).…”

Section: Discussionmentioning

confidence: 69%

“…When TPEN was added in repair media with zinc, it also chelated exocellular zinc, resulting in a decrease in the recovery ratio. Even if several other studies also reported the effects of certain ion cations on the recovery of sublethally injured bacteria (Busch and Donnelly 1992;Bi et al 2015;Shi et al 2017), to our best knowledge, the mechanism is still unknown. Zinc is an essential component of Cu/ZnSOD, which is one of three SODs in E. coli (Benov and Fridovich 1994).…”

Section: Discussionmentioning

confidence: 98%

“…In recent years, the resuscitation conditions of sublethally injured bacteria induced by various stresses were studied. For example, sublethally injured Escherichia coli induced by acidification, freezing or high‐pressure carbon dioxide can be completely repaired in rich medium trypticase soy broth (TSB), but can only be partly repaired in less nutritional media phosphate or PBS (Przybylski and Witter ; Bi et al ; Shi et al ). Moreover, the added agents affected resuscitation.…”

Section: Introductionmentioning

confidence: 99%

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Zinc mediates resuscitation of lactic acid‐injuredEscherichia coliby relieving oxidative stress

et al. 2019

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Aims Lactic acid is a natural antimicrobial in food industry, and also exists in fermented food. It was reported that sublethally injured Escherichia coli could survive in acidic conditions. When conditions become advantageous, injured E. coli can restore physiological function, which is a potential threat in food industry. Recovery is a necessary step for discriminating injured bacteria, but the resuscitation mechanism of injured bacteria is still unknown. Methods and Results In our study, sublethal lactic acid treatment (pH 4·2, 60 min) posed oxidative stress on E. coli by decrease of superoxide dismutase (SOD) activity and overproduction of reactive oxygen species (ROS). Zinc with low concentration (1·0 mmol l−1) significantly increased the recovery ratio of injured E.coli induced by lactic acid. The recovery ratios of injured cell in minimal A medium (minA) with 1·0 mmol l−1 zinc reached to that with 3·0 mmol l−1 catalase (CAT). Conversely, the addition of zinc chelator N, N, N′, N′‐tetrakis (2‐pyridylmethyl) decreased the recovery ratio. Zinc accelerated resuscitation of injured E. coli by improving SOD activity, and decreasing ROS production. Deletion of sodC encoding Cu/ZnSOD, katE/katG encoding CAT or regulating gene rpoS significantly decreased the recovery ratio. Among all of the mutants in this study, ΔrpoS and ΔsodC showed the lowest recovery ratio, which means they played significant roles in the process of resuscitation. Conclusion We provided direct evidence that zinc mediated resuscitation of lactic acid‐injured E. coli by relieving oxidative stress. Zinc can be used as a low‐cost and effective agent to improve recovery ratio and detection efficiency of injured bacteria. Significance and Impact of the Study Antibacterial agents are a challenge for bacteria, but bacteria can survive as a sublethally injured state under stresses. Using injured E. coli induced by lactic acid as a model organism, we validated the significant role of zinc on resuscitation of injured cells.

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Section: Introductionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Zinc mediates resuscitation of lactic acid‐injuredEscherichia coliby relieving oxidative stress

et al. 2019

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“…[14][15][16][17][18][19][20][21] Currently, most studies focus on the overexpression of cadmium resistant genes from Gram-positive bacteria to reverse cadmium pollution or to isolate strains in heavy metal contaminated areas: 2 cadA from S. aureus, 22 czc system from Alcaligenes eutrophus, 23 and MTS 16,23,24 and phsABC 25 from Salmonella typhimurium. In fact, E. coli is intrinsically tolerant to high levels of cadmium up to 0.9-1.0 mM.…”

Section: Introductionmentioning

confidence: 99%

Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)

Qin

Liu

et al. 2017

RSC Adv.

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Cadmium is recognized as one of the most toxic heavy metals, and chronic cadmium exposure threatens plant, animal and human health. Because certain bacteria can play an important role in remedying heavy metal pollution, it is essential to further our understanding and clone cadmium adsorption and resistance genes from microorganisms. In this study, the cadmium resistance of different Escherichia coli strains including BL21 (DE3), JM109, DH5a, Top10 and Mach was determined. Among those strains, the E. coli BL21 (DE3) exhibited the highest cadmium resistance. Sequence analyses of four cadmium resistant clones screened from the BL21 (DE3) fosmid library showed that the insertion of each fosmid plasmid contained a fragment located at the lambda phage DE3 region. Next, we overexpressed several genes of this fragment in BL21 (DE3) and identified that cadmium resistance of E. coli is acquired through the capB gene. Indeed, overexpression of the capB gene increased the adsorption rate of cadmium. Taken together, these results indicated that the capB gene confers E. coli BL21 (DE3) with increased cadmium resistance by adsorption of cadmium. This study provided new insight into the mechanism of microbial cadmium resistance and expanded our understanding of cadmium resistance, adsorption and bioremediation by microorganisms.

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Sublethal Injury Adaptation in Foodborne Pathogens

Khan

Sammi

Ahmad

et al. 2022

Stress Responses of Foodborne Pathogens

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Sublethal injury and recovery of Escherichia coli O157:H7 and K-12 after exposure to lactic acid

Cited by 25 publications

References 39 publications

Zinc mediates resuscitation of lactic acid‐injuredEscherichia coliby relieving oxidative stress

Zinc mediates resuscitation of lactic acid‐injuredEscherichia coliby relieving oxidative stress

Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)

Sublethal Injury Adaptation in Foodborne Pathogens

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