Sublethal effects of tributyltin oxide on the dogwhelk Nucella lapillus

Davies, Ian; Harding, M. James; Bailey, S.K.; Shanks, A. M.; Lange, Rogério Ribas

doi:10.3354/meps158191

Cited by 39 publications

(14 citation statements)

References 15 publications

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“…On the date of re-sampling, non-tagged adult dogwhelks (n = 40) were collected at the same areas for imposex determination. The relative penis size index (RPSI) is the mean bulk of the female penis (length 3 ) expressed as a percentage of the mean bulk of the male penis (length 3 ) in a sample (Davies et al 1997). The vas deferens stage index (VDSI), which is an index for the individual stage of the development of a vas deferens in the female, was determined as described by Gibbs et al (1987).…”

Section: Methodsmentioning

confidence: 99%

Growth rate as a factor confounding the use of the dogwhelk Nucella lapillus as biomonitor of heavy metal contamination

Leung¹,

Morgan²,

Wu³

et al. 2001

Mar. Ecol. Prog. Ser.

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Growth rate of individually tagged dogwhelks Nucella lapillus (L.) was measured in free-living individuals at 3 sites of differing heavy metal contamination in the Firth of Clyde, west Scotland. Condition index (CI), concentrations of metals (Cd, Cu, Pb and Zn), metallothionein (MT), RNA (the RNA/protein ratio) and glycogen were also measured. In general, the marine environments of Gourock and Largs were contaminated with significantly higher tributyltin, Pb and Zn than Loch Fyne, as indicated by the results of imposex indices, and metal concentrations in transplanted polymer-ligands (Chelex® 100) and Mytilus edulis. Further, metal concentrations of native M. edulis (Pb and Zn) and Semibalanus balanoides (Cu) from Gourock were significantly higher than those from Loch Fyne. However, metal accumulation in the dogwhelks displayed a very different pattern. At a standard size (0.5 g wet soft-body weight), N. lapillus from Largs showed higher Cd, Cu and MT in their tissues than individuals from the other 2 populations. Levels of Pb and Zn were similar among the populations despite different concentrations in Chelex and mussels. Gourock dogwhelks showed similar levels of Cu and MT but lower Cd compared to those of Loch Fyne. These differences can be attributed primarily to differences in dogwhelk growth rate between sites. Gourock individuals had a higher CI and RNA/protein ratio in the foot muscle and grew faster (especially at small sizes), resulting in a tissue-dilution effect on metal and MT concentrations. In contrast, higher levels of Cd, Cu and MT in dogwhelks from Largs can be attributed to their growth rate being relatively slow compared to the rate of metal accumulation. Slow-growing individuals in Loch Fyne had relatively high Cd, Pb Zn and MT, although Loch Fyne has been regarded as a clean reference site. Among populations, differences in growth rate may be due to differences in prey availability, predation pressure, and/or genotype. The present results demonstrate that inter-site differences in growth rate can confound the use of the dogwhelks as a biomonitor of metals.

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Section: Methodsmentioning

confidence: 99%

Growth rate as a factor confounding the use of the dogwhelk Nucella lapillus as biomonitor of heavy metal contamination

Leung¹,

Morgan²,

Wu³

et al. 2001

Mar. Ecol. Prog. Ser.

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“…Contraditoriamente, estudos realizados com as espécies H. trunculus (AXIAK et al, 2002) e N. lapillus (DAVIES et al, 1997) relatam a ocorrência de níveis naturais de imposex em populações desses prosobrânquios não expostas a contaminação por compostos orgânicos de estanho. A despeito desses estudos, sugere-se que a espécie S. rustica só apresentam imposex quando submetidas a tal contaminação uma vez que, em estações distantes das presumíveis fontes de COEs para o ambiente o imposex não ocorreu.…”

Section: Methodsunclassified

Altos índices de imposex em Stramonita rustica (Mollusca:Gastropoda) em áreas portuárias dos Estados de Alagoas e Sergipe, Brasil

Castro¹,

Braga²,

Rocha‐Barreira³

2005

TropOcean

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RESUMOO imposex é um excelente marcador da contaminação marinha por compostos orgânicos de estanho (COEs). Esse fenômeno consiste no surgimento de caracteres sexuais masculinos mensuráveis, em fêmeas de neogastrópodes. Um total de 17 estações de coleta foram escolhidas nas proximidades do terminal portuário de Jaraguá no Estado de Alagoas e no interior do estuário do Rio Sergipe, no Estado de Sergipe. Em ambas as áreas observa-se um intenso tráfego de embarcações. Em cada uma dessas estações foram coletados um total de 30 exemplares adultos de Stramonita rustica. Esses animais foram conduzidos ao laboratório e analisados quanto à presença do imposex revelando índices altamente elevados. A presença de imposex nessas amostras sugere fortemente que as referidas áreas estão contaminadas por COEs visto que o imposex só ocorre em animais expostos a esse tipo de contaminação. Palavras ABSTRACT HIGH IMPOSEX LEVELS IN Stramonita rustica (MOLLUSCA:GASTROPODA) IN HARBOR AREAS FROM ALAGOAS AND SERGIPE STATES, BRAZILImposex in gastropods is used worldwide as a bioindicator of organotin compounds (OTs). Imposex is the imposition of male secondary sexual characteristics in female neogastropods. Samples of 30 adult Stramonita rustica were collected in 17 sites from Jaraguá Harbor in Alagoas State and Sergipe River estuary in Sergipe State. These areas presented high shipping activities. The samples were led to the laboratory and were examined for occurrence of imposex. The imposex levels observed in samples were very high. The occurrence of imposex in S. rustica from harbor areas indicated organotin contamination. Key

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“…TBT and its degradation products will persist in aquatic sediments causing widespread contamination of the environment (Iwata et al, 1995;Tanabe, 1999;Antizar-Ladislao, 2008). The use of TBT, which is regulated internationally, can cause high toxicity, resulting in undesirable effects in non-target marine organisms, such as increase in shell malformation, imposex, and population decline in gastropods (Beaumont and Budd, 1984;Alzieu et al, 1986;Dyrynda, 1992;Davies et al, 1997). TBT is also toxic to mammalian cells (Antizar-Ladislao, 2008;Kotake, 2012).…”

Section: Introductionmentioning

confidence: 99%

Tributyltin induces cell cycle arrest at G1 phase in the yeast <i>Saccharomyces cerevisiae</i>

et al. 2014

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-Tributyltin (TBT) has long been recognized as a major environmental pollutant that can cause significant damage to the cellular functions as well as disruption of endocrine homeostasis. TBT induces apoptosis accompanied by production of reactive oxygen species (ROS) in mammalian and yeast cells. We observed that the budding yeast cells exposed to this compound at low concentrations exhibited cell growth arrest, but not cell death. Flow cytometric analysis of yeast cells without synchronization and morphological assessment of cells synchronized at M phase by nocodazole treatment indicated that TBT-exposed Saccharomyces cerevisiae cells were arrested at G1 phase of the cell cycle. This arrest was recovered by the addition of N-acetylcysteine, suggesting the involvement of ROS production by TBT. This is the first study to evaluate the action of TBT on cell cycle events.

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Sublethal effects of tributyltin oxide on the dogwhelk Nucella lapillus

Cited by 39 publications

References 15 publications

Growth rate as a factor confounding the use of the dogwhelk Nucella lapillus as biomonitor of heavy metal contamination

Growth rate as a factor confounding the use of the dogwhelk Nucella lapillus as biomonitor of heavy metal contamination

Altos índices de imposex em Stramonita rustica (Mollusca:Gastropoda) em áreas portuárias dos Estados de Alagoas e Sergipe, Brasil

Tributyltin induces cell cycle arrest at G1 phase in the yeast <i>Saccharomyces cerevisiae</i>

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