Sublethal effects of the flame retardant intermediate hexachlorocyclopentadiene (HCCPD) on the gene transcription and protein activity of Daphnia magna

Houde, Magali; Carter, Barbara; Douville, Mélanie

doi:10.1016/j.aquatox.2013.06.008

Cited by 36 publications

(10 citation statements)

References 25 publications

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“…The upregulation of digestive genes may be regarded as a compensation mechanism for reduced carbohydrate reserves (Soetaert et al, 2007). This was consistent with the results of the Houde et al (2013), in which the transcription of AMS genes was stimulated in the groups added with hexachlorocyclopentadiene (HCCPD). However, this phenomenon was contrary to the results of Zhao et al (2019), in which the transcription levels of AMS gene were inhibited in the groups with BDE-47, BDE-99 and their mixture.…”

Section: Enzyme Activitysupporting

confidence: 87%

“…And no signi cant correlation between gene transcription level and enzyme activity in trypsin. Houde et al (2013), showed that the activity of AMS was decreased signi cantly while D. magna exposed to HCCPD, and the gene transcription levels of AMS were decreased signi cantly. Schwarzenberger and Fink (2018) found that there was signi cant correlation between enzyme activity and gene transcription.…”

Section: Enzyme Activitymentioning

confidence: 98%

“…AMS are involved in brin and starch digestion in D. magna, while trypsin in protein digestion (Perera et al, 2012;Huang et al, 2017). All of them are the typical digestive enzymes (Houde et al, 2013). Figure 3A and 3B shows that the activities of AMS after 24 h of exposure and 24 h of recovery, respectively.…”

Section: Enzyme Activitymentioning

confidence: 98%

“…The nutritional enzyme gene was used as exploratory data to further analyze the possible cause of damage to D. magna caused by pollutants. Chronic exposure to hexachlorocyclopendiene (HCCDP), Houde et al (2013) used transcriptome tools to identify ve signi cant differential in genomic transcription related to metabolic function, including amylase and trypsin. Therefore, gene transcription involved in digestive enzyme synthesis was selected for evaluation based on the changes of amylase and trypsin in order to further explain the possible mode of action of pollutants to D. magna.…”

Section: Enzyme Activitymentioning

confidence: 99%

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Combined Toxicity and Toxicity Persistence of Antidepressants Citalopram and Mirtazapine to Zooplankton Daphnia Magna

Dong

et al. 2021

Preprint

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Citalopram (CTP) and mirtazapine (MTP) are two typical psychoactive drugs used for the depression treatment. As emerging pollutants, CTP and MTP has been given widely concern because they are active substances for organisms. Therefore, the ecotoxicological risks of aquatic organisms should be paid more attention to. In this study, the effects of CTP and MTP on the feeding behavior, heartbeat, nutritional enzymes and related gene transcriptions of Daphnia magna were investigated under single and mixed environmental stress. Meanwhile, the recovery of exposed D. magna was studied to analyze the toxic persistence of those pollutants. After 24-hexposure, the ingestion rate decreased by 34.2% and 21.5%, in the group of C-H and Mix-H respectively. After 24-h recovery, the feeding behavior of D. magna was stimulated by compensatory stimulation. In exposure period, the heartbeat rate of D. magna increased by 132.3%, 69%, 111.9%, 139.4%, and 92.4%, in the group of C-L, C-H, M-L, M-H and Mix-L respectively, and was recovered during the recovery period. The activity of α-amylase (AMS) and trypsin were significantly changed in most of the exposed daphnia, both in the exposure period and recovery period. CTP/MTP exposure stimulated transcription of the α-amylase gene. M-H and Mix-H exposure inhibited transcription of the trypsin gene and other stimulated transcriptions. After 24-h recovery, the stimulative or inhibitory effects were alleviated. There were different responses between gene transcription and enzyme activity. In conclusion, our results highlighted the toxic effects of single and mixed pollution of CTP and MTP on feeding, heartbeat, enzymes and genes of D. magna.

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Section: Enzyme Activitysupporting

confidence: 87%

Section: Enzyme Activitymentioning

confidence: 98%

Section: Enzyme Activitymentioning

confidence: 98%

Section: Enzyme Activitymentioning

confidence: 99%

See 2 more Smart Citations

Combined Toxicity and Toxicity Persistence of Antidepressants Citalopram and Mirtazapine to Zooplankton Daphnia Magna

Dong

et al. 2021

Preprint

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show abstract

“…3. AMS are involved in brin and starch digestion in D. magna, while trypsin in protein digestion (Perera et al, 2012;Huang et al, 2017), all of them are the typical digestive enzymes (Houde et al, 2013). Figure 3A and 3B shows that the activities of AMS after 24 h of exposure and 24 h of recovery, respectively.…”

Section: Enzyme Activitymentioning

confidence: 98%

Combined Toxicity and Toxicity Persistence of Antidepressants Citalopram and Mirtazapine to Zooplankton Daphnia Magna

Ma¹,

Li²,

Wei³

et al. 2021

Preprint

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Citalopram (CTP) and mirtazapine (MTP) are two typical psychoactive drugs used for the treatment of depression. As emerging pollutants, CTP and MTP are of widely concern because they are active substances for organisms. However, the studies about the toxicity potential of CTP/MTP pollution to aquatic organisms were limited. In the present study, the effects of CTP and MTP on the feeding behavior, heartbeat, nutritional enzymes and related gene expressions of Daphnia magna were investigated under single and mixed environmental stress. Meanwhile, the recovery of exposed D. magna was studied to analyze the toxic persistence of those pollutants. After 24 h of exposure, the ingestion rate decreased significantly under 1.45 mg/L of CTP. In the mixed treatment groups, no significant synergistic effect of CTM and MTP on daphnia’ feeding inhibition was found. After 24 h of recovery, the feeding behavior of D. magna was stimulated by compensatory stimulation. At exposure period, the heartbeat rate of exposed D. magna increased significantly, and was recovered during the recovery period. The activity of α-amylase (AMS) and trypsin, and their relative gene expression were significantly changed in most of the exposed daphnia, both in the exposure period and recovery period. But there were different responses between gene transcription with enzyme activity. In general, psychoactive drugs have an obvious toxic threat to aquatic organisms, and after acute exposure, the physiological function of D. magna could be recovered to a certain extent. The results were helpful to evaluate the ecological risk of psychotropic drugs in aquatic environments.

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De Novo Transcriptomic and Life-History Responses of Moina Micrura Under Stress Environment Conditions

et al. 2023

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Sublethal effects of the flame retardant intermediate hexachlorocyclopentadiene (HCCPD) on the gene transcription and protein activity of Daphnia magna

Cited by 36 publications

References 25 publications

Combined Toxicity and Toxicity Persistence of Antidepressants Citalopram and Mirtazapine to Zooplankton Daphnia Magna

Combined Toxicity and Toxicity Persistence of Antidepressants Citalopram and Mirtazapine to Zooplankton Daphnia Magna

Combined Toxicity and Toxicity Persistence of Antidepressants Citalopram and Mirtazapine to Zooplankton Daphnia Magna

De Novo Transcriptomic and Life-History Responses of Moina Micrura Under Stress Environment Conditions

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