Sublethal Effects of Insecticide Exposure on<i>Megacopta cribraria</i>(Fabricius) Nymphs: Key Biological Traits and Acetylcholinesterase Activity

Jin, Miao; Reisig, Dominic; Li, Guoping; Wu, Yuqing

doi:10.1093/jisesa/iew083

Cited by 14 publications

(5 citation statements)

References 30 publications

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“…However, the duration of the larval stage was not significantly affected by pesticide exposure at all concentrations. Similar results were observed by Miao, et al, (2016) who discovered a decrease in pupal weight and a prolonged pupal period. In addition, M. domestica adult emergence was reduced at imidacloprid doses below the lethal level.…”

Section: Fig (9 G1supporting

confidence: 88%

Efficiency of Insecticides Imidacloprid and Lambada-Cyhalothrin on Two Public Health Pests Musca domestica and Rattus norvegicus under Laboratory Conditions

M;,

Mohamed,

Elbath

2023

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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In the present study, imidacloprid and lambada-cyhalothrin insecticides were evaluated on second instar larvae of Musca domestica including mortality percentage, biological parameters, and histological effects under laboratory conditions. Five different concentrations (4, 2, 1, 0.5 and 0.25%) were used. The mortality data was recorded after 1,2,3,4, and 5 days post-treatment. The highest mortality was induced by imidacloprid and lambada-cyhalothrin (100.0 and 96.6%) after 5 days post-treatment at 4% concentration, respectively. Lethal concentrations (LC50) values in baits were: imidacloprid (0.13%) and lambada-cyhalothrin (0.30%). All concentrations of tested insecticides prolonged the duration of larval and pupal. Also, caused a reduction in the percentage of pupation and adult emergence compared to control. Pupal weight was also, significantly reduced. In general, fecundity decreased with the high concentration of insecticides. Insecticides caused histopathological and cytotoxic changes in the midgut of larvae. The two previous insecticides lambda Cyhalothrin and imidacloprid were also used against Rattus norvegicus males under laboratory conditions with different three concentrations (10, 5 and 0.30%) and (10, 5 and 0.13%) respectively. For two tested insecticides, the percentage of mortality reached 20 and 40%, respectively at 10% while these percentages were (10 and 30%) at 5% concentration, respectively. Also, 10% concentration had a great effect on the levels of AST, ALT, ALP, Urea and Creatine in the rat's blood. Histological tests were also conducted at a concentration of 5% on the stomach, liver and heart, it was found that some clear histological changes appeared as a result of treatment with these insecticides. Data suggested that imidacloprid and lambada-cyhalothrin can be used to help to control and eradicate Musca domestica and Rattus norvegicus especially imidacloprid insecticide had the highest effect in general on two tested public health pests.

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Section: Fig (9 G1supporting

confidence: 88%

Efficiency of Insecticides Imidacloprid and Lambada-Cyhalothrin on Two Public Health Pests Musca domestica and Rattus norvegicus under Laboratory Conditions

M;,

Mohamed,

Elbath

2023

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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“…A previous study found that an appropriate concentration of imidacloprid had a certain positive effect on silkworms and that a concentration of this pesticide less than or higher than this amount inhibits silkworms [ 29 ]. The fertility of Sitobion avenae feeding on wheat plants treated with LC 10 (0.0053 mg/L) of imidacloprid, dinotefuran, thiacloprid and thiamethoxam is not affected [ 32 ]. Studies have found that thiamethoxam at a sublethal concentration has no obvious stimulating effect on the adult longevity of aphids and can be used as a permitted level for crop application [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Sublethal Effects of Neonicotinoid Insecticides on the Development, Body Weight and Economic Characteristics of Silkworm

Chen

Sun

Yang

et al. 2023

Toxics

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Silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae) is a critical insect for silk producers, but the inappropriate application of insecticides negatively affects the physiology and behavior of silkworms. This study found that the effects of neonicotinoid insecticides applied using two spraying methods on the growth and development of silkworms were different: the median lethal concentration (LC50) values of two pesticides applied using the leaf-dipping method were 0.33 and 0.83 mg L−1 and those of two pesticides applied using the quantitative spraying method were 0.91 and 1.23 mg kg−1. The concentration of pesticides on the mulberry leaves did not decrease after their application using the quantitative spraying method, and a uniform spraying density was observed after the mulberry leaves were air-dried (no liquid) under realistic conditions. We then treated silkworms with the quantitative spraying method and leaf-dipping method. The treatment of silkworm larvae with imidacloprid and thiamethoxam at sublethal concentrations significantly prolonged the development time and significantly decreased the weight and pupation rate, as well as economic indicators of enamel layers and sputum production. Thiamethoxam treatment significantly increased the activities of carboxylesterase (CarE) and glutathione-S-transferase (GST). The activity of CarE and GST increased, decreased, and then increased, and the highest activity was detected on the 10th and 12th days. Thiamethoxam exposure significantly elevated the transcription levels of CarE-11, GSTe3 and GSTz2 and induced DNA damage in hemocytes. This study confirmed that the quantitative spray method is more stable than the leaf-dipping method. Moreover, imidacloprid and thiamethoxam treatment affected the economy and indexes of silkworms and induced changes in detoxification enzymes and DNA damage in silkworms. These results provide a basis for understanding the mechanism of the sublethal effects of insecticides on silkworms.

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“…Point mutations in insect ace genes usually result in insecticide resistance ( Lee et al 2015 ). Therefore, these genes have attracted much attention in insecticide toxicological studies ( Alizadeh et al 2014 , Miao et al 2016 , Zhou et al 2016 ). Recently, it was shown that knockdown of ace1 genes led to increased sensitivity of insects to AChE insecticides ( Revuelta et al 2009 , 2011 , Lu et al 2012b ), and that silencing ace1 and/or ace2 impacted insect development ( He et al 2012 , Lu et al 2012b , Salim et al 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Expression Analysis of Two Acetylcholinesterase Genes From the Small White Butterfly Pieris rapae (Lepidoptera: Pieridae)

Jiang

Liu

2018

Journal of Insect Science

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Acetylcholinesterases (AChEs) are essential for the hydrolysis of the neurotransmitter acetylcholine and play crucial roles in the termination of neurotransmission. AChEs are encoded by the ace genes. However, the ace genes from the small white butterfly, Pieris rapae (L.) (Lepidoptera: Pieridae), remained uncharacterized. In this study, two aces (Prace1 and Prace2) were identified from P. rapae. Prace1 encoded a PrAChE1 protein consisting of 694 amino acid residues, and Prace2 encoded the 638-amino-acid PrAChE2. The two identified PrAChEs both had features typical of AChEs, including the catalytic triad, choline-binding sites, an oxyanion hole, an acyl pocket, a peripheral anionic subsite, an FGESAG motif and 14 conserved aromatic amino acids. Phylogenetic analysis showed that Prace1 and Prace2 were clustered into two distinct groups: ace1 and ace2, respectively. The two Praces were distributed on different genomic scaffolds: Prace1 on scaffold 156 and Prace2 on scaffold 430. Additionally, Prace1 consisted of three exons and two introns, whereas Prace2 consisted of six exons and five introns. One amino acid mutation (Gly324Ala) in PrAChE1 and two (Ser291Gly and Ser431Phe) in PrAChE2 were consistent with mutations in other insect AChEs that are associated with insecticide insensitivity. Both Prace1 and Prace2 were highly expressed at the fifth-instar larval stage and in the larval head, and the transcriptional levels of Prace1 were significantly higher than those of Prace2 in all of the tested life stages and tissues. This is the first report characterizing two ace genes in P. rapae. The results pave the way for functional study of these genes.

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Sublethal Effects of Insecticide Exposure onMegacopta cribraria(Fabricius) Nymphs: Key Biological Traits and Acetylcholinesterase Activity

Cited by 14 publications

References 30 publications

Efficiency of Insecticides Imidacloprid and Lambada-Cyhalothrin on Two Public Health Pests Musca domestica and Rattus norvegicus under Laboratory Conditions

Efficiency of Insecticides Imidacloprid and Lambada-Cyhalothrin on Two Public Health Pests Musca domestica and Rattus norvegicus under Laboratory Conditions

Sublethal Effects of Neonicotinoid Insecticides on the Development, Body Weight and Economic Characteristics of Silkworm

Molecular Characterization and Expression Analysis of Two Acetylcholinesterase Genes From the Small White Butterfly Pieris rapae (Lepidoptera: Pieridae)

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