Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.

Wu, Yanyan; Luo, Qin; Hou, Chunsheng; Wang, Andrew Y.; Dai, Pingli; Gao, Jing; Liu, Yong-Jun; Diao, Qingyun

doi:10.1038/s41598-017-16245-0

Cited by 45 publications

(44 citation statements)

References 44 publications

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“…Regarding proteins related to the motor function of bees, it was observed that after eight days of contamination with sublethal concentrations of imidacloprid A. mellifera climbing ability was significantly reduced. Analyzes have shown that genes related to muscle function were downregulated, confirming the mobility impairment (Wu et al, 2017b). Motor alterations have also been reported in laboratory trials with sublethal doses of imidacloprid (Lunardi et al, 2017 andBovi et al, 2018).…”

Section: Imidaclopridmentioning

confidence: 58%

Sublethal effects of neonicotinoids in bees: a review

2020

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Beginning in 2006, beekeepers on the east coast of the United States began to report severe declines in their honeybee colonies. Because of the severity and unusual circumstances of these declines, scientists have called this phenomenon colony collapse disorder (CCD). In 2019, 500 million dead bees were found in Brazil. Analyzes of dead bees identified agrochemicals in approximately 80% of them. Thus, it is believed that one of the main causes for CCD is the intensive use of agrochemicals. Neonicotinoids are the most widely used class of insecticides in the world, they are used for pest control in a variety of crops. However, they can not only affect insects considered pests, but also non-target organisms, such as pollinators. This class of insecticides is divided into five main active ingredients: imidacloprid, thiamethoxam, clothianidin, thiacloprid and acetamiprid. Several studies demonstrate that sublethal concentrations of these insecticides affect bees in different ways, such as navigation memory and muscle movements. Thus, this review aims to report the studies published between 2014 and 2019 regarding the contamination of bees with sublethal doses of the five active ingredients of the neonicotinoid class. Imidacloprid and thiamethoxam are the most used insecticides of this class and show high toxicity to bees. On the other hand, clothianidin showed the least sublethal effects on bees on the studies reported on this review. Thiacloprid and acetamiprid, although less used in agriculture, also impair several aspects of bee health. Thus, it is possible to infer that neonicotinoids are contributing to the disappearance of bees worldwide.

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Section: Imidaclopridmentioning

confidence: 58%

Sublethal effects of neonicotinoids in bees: a review

2020

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“…the mushroom bodies and antennal lobes; Barbara et al, 2008;Deglise et al, 2002;Palmer et al, 2013). It was recently found, however, that honeybees exposed to imidacloprid at 10 ppb showed significant down-regulation of muscle-related genes (Wu et al, 2017). This down-regulation, in addition to the blockage of nAChRs, may underlie the subtle motor deficits observed here and elsewhere.…”

Section: Discussionmentioning

confidence: 69%

Imidacloprid impairs performance on a model flower handling task in bumblebees (Bombus impatiens)

2020

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Bumblebees exposed to neonicotinoid pesticides collect less pollen on foraging trips. Exposed bumblebees are also slower to learn to handle flowers, which may account for reduced pollen collection. It is unclear, however, why neonicotinoid exposure slows learning to handle flowers. I investigated the effect of imidacloprid, a neonicotinoid pesticide, on bumblebee motor learning using a lab model of flower handling. Bumblebees learned to invert inside a narrow tube and lift a petal-shaped barrier to reach a reward chamber. Imidacloprid-exposed bumblebees showed a dose-dependent delay to solve the task, which resulted from reduced switching between behavioural strategies and a subsequent delay in use of the successful strategy. This effect was consistent in colonies exposed at 10 but not 2.6 ppb, suggesting a variable effect on individuals at lower doses. These results help to explain why exposed bumblebees are slow to learn to handle flowers and collect less pollen on foraging trips.

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“…IMI may remain on the water surface for a long time due to high water solubility [70]. IMI cannot be hydrolyzed by ACh esterase, causing interruption to nAChR leading to long-lasting effects, such as loss of reflexes, muscular weakness, and paralysis [71]. Consequently, it alters invertebrate movements and leads to starvation and death.…”

Section: Discussionmentioning

confidence: 99%

Exploiting the Freshwater Shrimp Neocaridina denticulata as Aquatic Invertebrate Model to Evaluate Nontargeted Pesticide Induced Toxicity by Investigating Physiologic and Biochemical Parameters

et al. 2021

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As a nicotinoid neurotoxic insecticide, imidacloprid (IMI) works by disrupting nerve transmission via nicotinic acetylcholine receptor (nAChR). Although IMI is specifically targeting insects, nontarget animals such as the freshwater shrimp, Neocaridina denticulata, could also be affected, thus causing adverse effects on the aquatic environment. To investigate IMI toxicity on nontarget organisms like N. denticulata, their physiology (locomotor activity, heartbeat, and gill ventilation) and biochemical factors (oxidative stress, energy metabolism) after IMI exposure were examined. IMI exposure at various concentrations (0.03125, 0.0625, 0.125, 0.25, 0.5, and 1 ppm) to shrimp after 24, 48, 72 h led to dramatic reduction of locomotor activity even at low concentrations. Meanwhile, IMI exposure after 92 h caused reduced heartbeat and gill ventilation at high concentrations. Biochemical assays were performed to investigate oxidative stress and energy metabolism. Interestingly, locomotion immobilization and cardiac activity were rescued after acetylcholine administration. Through molecular docking, IMI demonstrated high binding affinity to nAChR. Thus, locomotor activity and heartbeat in shrimp after IMI exposure may be caused by nAChR blockade and not alterations caused by oxidative stress and energy metabolism. To summarize, N. denticulata serves as an excellent and sensitive aquatic invertebrate model to conduct pesticide toxicity assays that encompass physiologic and biochemical examinations.

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Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.

Cited by 45 publications

References 44 publications

Sublethal effects of neonicotinoids in bees: a review

Sublethal effects of neonicotinoids in bees: a review

Imidacloprid impairs performance on a model flower handling task in bumblebees (Bombus impatiens)

Exploiting the Freshwater Shrimp Neocaridina denticulata as Aquatic Invertebrate Model to Evaluate Nontargeted Pesticide Induced Toxicity by Investigating Physiologic and Biochemical Parameters

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