Sublethal ammonia induces alterations of emotions, cognition, and social behaviors in zebrafish (Danio rerio)

Lin, Li‐Yih; Horng, Jiun-Lin; Cheng, Chieh-An; Chang, Chun-Yung; Cherng, Bor-Wei; Liu, Sian-Tai; Chou, Ming-Yi

doi:10.1016/j.ecoenv.2022.114058

Cited by 10 publications

(1 citation statement)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mortality caused by ammonia exposure was mainly attributed to the activation of N-methyl-D-aspartate (NMDA) type glutamate receptor and the influx of excessive Ca 2+ to induce cell death in the central nervous system [ 8 ]. In zebrafish, messenger RNA expressions of glutaminase and glutamate dehydrogenase in the brain were induced, suggesting that ammonia exposure altered glutamate neurotransmitters [ 9 ]. Similarly, ammonia stress can disrupt the endocrine and neurotransmitter systems of Takifugu rubripes [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Liver Injury and Metabolic Dysregulation in Largemouth Bass (Micropterus salmoides) after Ammonia Exposure

Zou

Wang

et al. 2023

Metabolites

View full text Add to dashboard Cite

Elevated environmental ammonia leads to respiratory disorders and metabolic dysfunction in most fish species, and the majority of research has concentrated on fish behavior and gill function. Prior studies have rarely shown the molecular mechanism of the largemouth bass hepatic response to ammonia loading. In this experiment, 120 largemouth bass were exposed to total ammonia nitrogen of 0 mg/L or 13 mg/L for 3 and 7 days, respectively. Histological study indicated that ammonia exposure severely damaged fish liver structure, accompanied by increased serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activity. RT-qPCR results showed that ammonia exposure down-regulated the expression of genes involved in glycogen metabolism, tricarboxylic acid cycle, lipid metabolism, and urea cycle pathways, whereas it up-regulated the expression of genes involved in gluconeogenesis and glutamine synthesis pathways. Thus, ammonia was mainly converted to glutamine in the largemouth bass liver during ammonia stress, which was rarely further used for urea synthesis. Additionally, transcriptome results showed that ammonia exposure also led to the up-regulation of the oxidative phosphorylation pathway and down-regulation of the mitogen-activated protein kinase signaling pathway in the liver of largemouth bass. It is possible that the energy supply of oxidative phosphorylation in the largemouth bass liver was increased during ammonia exposure, which was mediated by the MAPK signaling pathway.

show abstract

Section: Introductionmentioning

confidence: 99%