Sublethal acid stress and uncoupling effects on cell growth and product formation in Xanthomonas campestris cultures

Esgalhado, M.Eugénia; Caldeira, Ana Teresa; Roseiro, J. Carlos; Emery, A. N.

doi:10.1016/s1369-703x(02)00070-0

Cited by 9 publications

(9 citation statements)

References 22 publications

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“…In the presence of low concentrations of acid, metabolically uncoupled cells should maintain a rapid rate of substrate oxidation, which will certainly lead to an excess of carbon in the cell. Under these conditions, the cell needs to avoid the accumulation of metabolites and exocellular products export can appear as a mechanism of carbon dissipation [19,20]. This is consistent with lactate lower rate of decrease under acid stress although the profile of lactate synthesis is closely associated with microbial growth.…”

Section: Acid Stress Effects On Growth Curvesupporting

confidence: 64%

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The effect of acid stress on lactate production and growth kinetics in Lactobacillus rhamnosus cultures

Yáñez

Marques

Gı́rio

et al. 2008

Process Biochemistry

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Section: Acid Stress Effects On Growth Curvesupporting

confidence: 64%

“…This is a feeble adaptation solution, as in some bacteria, e.g. those producing polymers, the level of dissipation in acid conditions leads to an over production of the product [19,20].…”

Section: Acid Toxicity In L Rhamnosus Culturesmentioning

confidence: 99%

The effect of acid stress on lactate production and growth kinetics in Lactobacillus rhamnosus cultures

Yáñez

Marques

Gı́rio

et al. 2008

Process Biochemistry

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“…The results in accordance with Esgalhado's report [12] that weak acid inhabitation on bacteria increased with the concentration at a certain external pH. Some researchers consider that uncoupling effects lead to inhabitation [13]. Fig.…”

Section: Resultssupporting

confidence: 90%

Xanthan production with the hydrolysate of wheat straw

Zhang¹

2015

Proceedings of the 2015 6th International Conference on Manufacturing Science and Engineering

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Hydrolysate of steam-exploded wheat straw (SEWS) was used as the carbon source of xanthan production. The effects of common inhibitors generated during steam explosion on xanthan production were evaluated; a combine method was used to improve the fermentability of enzymatic hydrolysate of SEWS. All the three groups of compounds (weak acid, furan derivatives and phenolic compounds) inhibited xanthan generation; furan derivatives and phenolic compounds could be reduced by using the combinations of low steam pressure and long maintain time; flocculation was effective to remove phenolic compounds; weak acid and furan derivatives could be removed when SEWS was air-dried. Using the detoxified hydrolysate of SEWS, the xanthan production attained 20.7 g L-1 and the yield was 0.62 g xanthan per g sugar (glucose and xylose). It was concluded that SEWS is a potential carbon source of xanthan fermentation.

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“…The YM medium is internationally described as growth medium for X. campestris [10][11][12][18][19][20]. However it is expensive (US $248.00/Kg) to be used in the production of xanthan gum wich costs on average US $5.5/kg [21,22].…”

Section: Introductionmentioning

confidence: 99%

New Culture Medium to Xanthan Production by Xanthomonas campestris pv. campestris

et al. 2011

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Xanthan is a important biopolymer for commercial purpose and it is produced in two stages by Xanthomonas campestris. In the first one, the bacterium is cultivated in the complex medium enriched in nitrogen and the biomass produced is used as inoculum for the next stage in which the gum is produced in another medium. In this work a new medium for the first stage is proposed in place of currently used YM medium. Different formulated growth media were studied and the correspondent biomass produced was analysed as inoculum for the second stage. The inoculum and gum were produced by batch process in shaker at 27°C in pH 6.0 and at 30°C in pH 7.0, respectively. The gum was precipitated with ethanol (3:1 v/v). The dryed biomass and xathan gum produced were determined by drying in oven at 105 and 40°C, respectively. The viscosity of the fermentation broth and 1% gum solution in water were determined in Brookfield viscometer. The formulated medium presented the increase in gum production (30%), broth (136%) and 1% gum solution viscosity (60%) compared to YM, besides the inferior cost. The results showed the importance of the quality of the inoculum from the first stage of the culture which influenced on the gum viscosity in the second stage.

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Sublethal acid stress and uncoupling effects on cell growth and product formation in Xanthomonas campestris cultures

Cited by 9 publications

References 22 publications

The effect of acid stress on lactate production and growth kinetics in Lactobacillus rhamnosus cultures

The effect of acid stress on lactate production and growth kinetics in Lactobacillus rhamnosus cultures

Xanthan production with the hydrolysate of wheat straw

New Culture Medium to Xanthan Production by Xanthomonas campestris pv. campestris

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