Subklinik Beyaz Kas Hastalıklı Kuzularda Homosistein, Troponin I ve Nitrik Oksit Düzeylerinin Değerlendirilmesi

Abstract

SummaryWhite muscle disease (WMD) or Nutritional myodegeneration disease (NMD) is a degenerative disease of the cardiac and skeletal muscles. The level of serum activity of Plasma homocysteine (Hcy), cardiac troponin I (cTn I), Nitric oxide (NO) were investigated in lambs with subclinical NMD in this study. Ten healthy lambs and twenty lambs with subclinical NMD were used in this study. The blood samples were firstly taken at the onset of the disease and then 3 and 10 days after treatment in NMD, and taken once control group. The values of serum activity of creatin kinase (CK), aspartate aminotranferase (AST), alanine aminotranferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were measured with autoanalyzer. Plasma concentration of Hcy, serum cTn I, and levels of NO were determined with ELISA method and value of activity erythrocyte glutathione peroxidase (GSH-Px) was measured with spectrophotometer. Before treatment, values of plasma Hcy, serum NO, cTn I, AST, ALT, ALP, CK, and LDH in lambs with NMD were higher than those of healthy ones (P<0.001), while GSH-Px activity of lambs with NMD are lower than those of healthy (P<0.001). After ten days of treatment, there were no significant diff erences between treated lambs with NMD and healthy lambs. Nutritional myodegeneration in lambs is associated with increased levels of Hcy, cTn I, and NO. Increased levels of Hcy, cTn I, and NO may be a result of selenium deficiency causing myocardial disorder in NMD lambs. Keywords: Nutritional Myodegeneration, Lamb, Homocysteine, Troponin I, Nitric oxide Subklinik Beyaz Kas Hastalıklı Kuzularda Homosistein, Troponin I ve Nitrik Oksit Düzeylerinin Değerlendirilmesi ÖzetBeyaz kas hastalığı veya besinsel kas dejenerasyonları, hayvanlarda iskelet ve kalp kaslarının dejeneratif değişiklikleri ile karakterize bir hastalığıdır. Bu çalışmada kuzulardaki subklinikal beyaz kas hastalığında plazma homosistein (Hcy), serum kardiak troponin I (cTnI) ve nitrik oksit (NO) düzeyleri araştırıldı. Çalışmada 10 sağlıklı ve 20 subklinikal beyaz kas hastalıklı kuzu kullanıldı. Kan örnekleri NMD kuzularda tedavi öncesi, tedavi sonrası 3. ve 10. günlerde ve kontrol grubunda bir kez alındı. Serum kreatinin kinaz (CK), aspartat aminotransferaz (AST), alanin aminotranferaz (ALT), alkalin fosfataz (ALP) ve laktat dehidrogenaz (LDH) değerleri otoanalizatör cihazıyla ölçüldü. Plazma homosistein konsantrasyonu, serum cTn I ve NO düzeyleri ELİSA cihazıyla ve eritrosit glutathione peroxidase (GSH-Px) aktivitesi ise spektrofotometre cihazıyla ölçüldü. Tedavi öncesi, kuzuların subklinik beyaz kas hastalığında GSH-Px aktiviteleri sağlıklı kuzulara göre daha düşük iken (P<0.001), plazma Hcy, serum NO, cTn I, AST, ALT, ALP, CK ve LDH değerleri sağlıklı kuzuların değerlerinden yüksek bulundu (P<0.001). Tedavi sonrası 10. günde elde edilen ortalama değerler, kontrol grubunun değerlerinden farklı değildi. Kuzuların subklinik beyaz kas hastalığında Hcy, cTn I ve NO değerlerindeki yükselmelerin, selenyum eksikliğinin sebep olduğu miyokardiyal ...

Evaluation of local expressions of acute phase proteins in white muscle disease in lambs by the immunohistochemical method

“…In addition, increased reactive oxygen species (ROS) levels serve as markers for oxidative stress, and lipid peroxidation and instability of the redox system are associated [13]. In many studies, it has been reported that oxidative stress plays a very important role in the pathogenesis of WMD [4,13,14,15]. In this context, oxidative stress is seen as an important cause of degenerative and necrotic changes in related tissues.…”