Suberoylanilide hydroxamic acid induced microspore embryogenesis and promoted plantlet regeneration in ornamental kale (Brassica oleracea var. acephala)

Liu, Chuanhong; Song, Gengxing; Fang, Bing; Li, Yong; Zou, Jiaqi; Dong, Shiyao; Du, Sai; Ren, Jie; Feng, Hui

doi:10.1007/s00709-022-01764-z

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Cut flower ornamental kale is mainly distributed in temperate regions, and is planted more in the United Kingdom, the Netherlands, Germany, and the United States. The ornamental period on land is over 4 months since its strong cold resistance and easy cultivation [1]. Cut flower Ornamental Kale contains a large amount of anthocyanins, which as effective antioxidants have the potential to prevent some cancers, cardiovascular diseases and other chronic diseases [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

et al. 2022

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Cut flower ornamental kale (Brassica oleracea var. acephala) is a biennial cultivar, which completes a sexual reproductive generation in two years. Isolated microspore culture (IMC) can accelerate plant homozygosity instead of self-pollinations. However, the application of IMC in cut flower ornamental kale was rare since its low rate of embryogenesis. It is proved that histone acetylation might affect the gene expression in microspores and led to the transformation of microspores from pollen development pathway to embryogenesis. In this paper, microspores, derived from three varieties of cut flower ornamental kale, Crane Bicolor (CB), Crane Pink (CP) and Crane Feather Queen (CFQ), were treated with histone deacetylation inhibitor (HDACI) trichostatin A (TSA). Results revealed that the appropriate concentration of TSA was 10 nM for CB with obtaining 5.39 embryos per bud, while for CP and CFQ was 5 nM with acquiring 10.89 and 16.99 embryos per bud, respectively. TSA treatment also reduced the embryonic mortality, of which 10 nM TSA treatments CB was the optimal and the embryonic mortality decreased to 25.01%. The double haploid (DH) proportion of regenerated plants reached 37.3%. These results contribute to improving the technology for IMC in cut flower ornamental kale.

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Section: Introductionmentioning

confidence: 99%

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

et al. 2022

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“…Histone acetylation status affects a wide range of developmental processes, including stress response (Luo et al, 2017) and cell division and differentiation (Bie et al, 2020). In different species, HDAC inhibitors, including trichostatin A (TSA), have been shown to increase the proportion of microspores that change their developmental fate to embryogenesis (Castillo et al, 2020; Jiang et al, 2017; Liu et al, 2022; Pandey et al, 2017; Wang et al, 2019; Zhang et al, 2016), suggesting that HDAC‐mediated pathways play similar roles in stress‐induced microspore embryogenesis in different species. This includes the low response B. napus DH12075 line, where a combination of HS + 0.05 μM TSA dramatically increases both the proportion of embryogenic structures and the rate of differentiated embryo production (Corral‐Martinez et al, 2020; Li et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The different response of Brassica napus genotypes to microspore embryogenesis induced by heat shock and trichostatin A is not determined by changes in cell wall structure and composition but by different stress tolerance

Camacho‐Fernández,

Corral‐Martínez,

Calabuig‐Serna

et al. 2024

Physiologia Plantarum

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During microspore embryogenesis, microspores are induced to develop into haploid embryos. In Brassica napus, microspore embryogenesis is induced by a heat shock (HS), which initially produces embryogenic structures with different cell wall architectures and compositions, and with different potentials to develop into embryos. The B. napus DH4079 and DH12075 genotypes have high and very low embryo yields, respectively. In DH12075, embryo yield is greatly increased by combining HS and the histone deacetylase (HDAC) inhibitor trichostatin A (TSA). However, we show that HS + TSA inhibits embryogenesis in the highly embryogenic DH4079 line. To ascertain why TSA has such different effects in these lines, we treated DH4079 and DH12075 microspore cultures with TSA and compared the cell wall structure and composition of the different embryogenic structures in both lines, specifically the in situ levels and distribution of callose, cellulose, arabinogalactan proteins and high and low methyl‐esterified pectin. For both lines, HS + TSA led to the formation of cell walls unfavorable for embryogenesis progression, with reduced levels of arabinogalactan proteins, reduced cell adhesion of inner walls and altered pectin composition. Thus, TSA effects on cell walls cannot explain their different embryogenic response to TSA. We also applied TSA to DH4079 cultures at different times and concentrations before HS application, with no negative effects on embryogenic induction. These results indicate that DH4079 microspores are hypersensitive to combined TSA and HS treatments, and open up new hypotheses about the causes of such hypersensitivity.

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Thidiazuron Promoted Microspore Embryogenesis and Plant Regeneration in Curly Kale (Brassica oleracea L. convar. acephala var. sabellica)

et al. 2023

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Curly kale (Brassica oleracea L. convar. acephala var. sabellica), the most common type of edible kale, characterized by providing rich nutrition and health care functions, is sought after and has been listed as top of the healthiest vegetables in recent trends, and has aroused the interest of breeders in cultivating new varieties. However, it usually takes more than six years to obtain a homozygous kale inbred line for commercial seed production through conventional breeding procedures due to its long growth and development period. The isolated microspore culture (IMC) technique could be a time-saving alternative method for producing doubled haploid (DH) lines that are genetically homozygous. In this study, we successfully utilize the efficient cytokinin thidiazuron (TDZ) to promote microspore embryogenesis and plant regeneration in two curly kale cultivars (‘Winterbor F2’ and ‘Starbor F2’). Compared with the control (0 mg/L TDZ), all tested TDZ concentrations (0.1, 0.2, 0.3, 0.4 mg/L) had no adverse effects on embryogenesis, and 0.2 mg/L TDZ had an optimal effect on embryo survival and plant regeneration of the two genotypes. For ‘Starbor F2’, 0.2 mg/L TDZ treatment achieved the highest embryogenesis rate (1.83-fold higher than the control group) and direct seeding rate (1.61-fold increase), and the lowest mortality rate. Likewise, 0.2 mg/L TDZ increased the embryogenesis rate of ‘Winterbor F2’ by 1.62 times, the direct seeding rate by 1.61 times, and the mortality rate fell to the lowest. A 1/2 Murashige and Skoog (MS) medium with 0.2 mg/L 1-Naphthaleneacetic acid (NAA) can significantly promote the rooting of the regenerated seedlings. These results provide new insights into the practical application of the IMC technique in shortening the breeding cycle of kale.

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Suberoylanilide hydroxamic acid induced microspore embryogenesis and promoted plantlet regeneration in ornamental kale (Brassica oleracea var. acephala)

Cited by 3 publications

References 16 publications

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (Brassica oleracea var. acephala)

The different response of Brassica napus genotypes to microspore embryogenesis induced by heat shock and trichostatin A is not determined by changes in cell wall structure and composition but by different stress tolerance

Thidiazuron Promoted Microspore Embryogenesis and Plant Regeneration in Curly Kale (Brassica oleracea L. convar. acephala var. sabellica)

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