Suberin from Plants

Kolattukudy, Pappachan E.

doi:10.1002/3527600035.bpol3a02

Cited by 26 publications

(51 citation statements)

References 111 publications

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“…Similar structure models have been proposed for the aliphatic domain of suberin, that is also characterized by substantial amounts of diacids and a moderate content of secondary functional groups (Kolattukudy, 2001b;Bernards, 2002). This unusual, rather suberin-like a,x-diacid content in the cutin might be unique to Arabidopsis, but could also bee a biochemical property of very thin cuticular membranes.…”

Section: The Arabidopsis Cutin Is Unusually Rich In Axdicarboxylic Amentioning

confidence: 83%

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Apoplastic polyesters in Arabidopsis surface tissues – A typical suberin and a particular cutin

et al. 2005

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Cutinized and suberized cell walls form physiological important plant-environment interfaces as they act as barriers limiting water and nutrient loss and protect from radiation and invasion by pathogens. Due to the lack of protocols for the isolation and analysis of cutin and suberin in Arabidopsis, the model plant for molecular biology, mutants and transgenic plants with a defined altered cutin or suberin composition are unavailable, causing that structure and function of these apoplastic barriers are still poorly understood. Transmission electron microscopy (TEM) revealed that Arabidopsis leaf cuticle thickness ranges from only 22 nm in leaf blades to 45 nm on petioles, causing the difficulty in cuticular membrane isolation. We report the use of polysaccharide hydrolases to isolate Arabidopsis cuticular membranes, suitable for depolymerization and subsequent compositional analysis. Although cutin characteristic xhydroxy acids (7%) and mid-chain hydroxylated fatty acids (8%) were detected, the discovery of a,x-diacids (40%) and 2-hydroxy acids (14%) as major depolymerization products reveals a so far novel monomer composition in Arabidopsis cutin, but with chemical analogy to root suberin. Histochemical and TEM analysis revealed that suberin depositions were localized to the cell walls in the endodermis of primary roots and the periderm of mature roots of Arabidopsis. Enzyme digested and solvent extracted root cell walls when subjected to suberin depolymerization conditions released x-hydroxy acids (43%) and a,x-diacids (24%) as major components together with carboxylic acids (9%), alcohols (6%) and 2-hydroxyacids (0.1%). This similarity to suberin of other species indicates that Arabidopsis roots can serve as a model for suberized tissue in general.

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Section: The Arabidopsis Cutin Is Unusually Rich In Axdicarboxylic Amentioning

confidence: 83%

“…Suberin is a more complex biopolymer composed of an aliphatic and an aromatic domain (Kolattukudy, 1980(Kolattukudy, , 2001bBernards and Lewis, 1998). The latter is primarily composed of p-coumaric and ferulic acid, presumably involved in linking the aliphatic domain to cell wall polysaccharides (Bernards, 2002).…”

Section: Introductionmentioning

confidence: 99%

Apoplastic polyesters in Arabidopsis surface tissues – A typical suberin and a particular cutin

et al. 2005

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“…Cutin is the aliphatic polyester that is present in the plant cuticle (Kolattukudy, 2001a;Pollard et al, 2008;Li-Beisson et al, 2013). Suberin is an aliphatic polyester that is deposited in a broad spectrum of diffusion barriers, particularly those that form in the roots and in the periderm of shoots (Kolattukudy, 2001b). In cutin, the amount of aromatic components is very low, but this proportion is higher in suberin (Kolattukudy, 2001b;Pollard et al, 2008;Beisson et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Apoplastic Diffusion Barriers in Arabidopsis

Nawrath

Schreiber

Franke

et al. 2013

The Arabidopsis Book

129

143

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During the development of Arabidopsis and other land plants, diffusion barriers are formed in the apoplast of specialized tissues within a variety of plant organs. While the cuticle of the epidermis is the primary diffusion barrier in the shoot, the Casparian strips and suberin lamellae of the endodermis and the periderm represent the diffusion barriers in the root. Different classes of molecules contribute to the formation of extracellular diffusion barriers in an organ-and tissue-specific manner. Cutin and wax are the major components of the cuticle, lignin forms the early Casparian strip, and suberin is deposited in the stage II endodermis and the periderm. The current status of our understanding of the relationships between the chemical structure, ultrastructure and physiological functions of plant diffusion barriers is discussed. Specific aspects of the synthesis of diffusion barrier components and protocols that can be used for the assessment of barrier function and important barrier properties are also presented.

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“…Therefore, glycerol most probably enables the threedimensional network of the suberin structure. As suberin is attached to the cell wall, it is virtually impossible to isolate it in pure and native state, which further complicates structural studies (1)(2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

“…The quantitative composition of monomeric mixtures obtained depends on the method, but consists mostly of long-chain (16-24 carbon atoms) aliphatic dicarboxylic acids, ω-hydroxy fatty acids, very long chain (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) carbons) fatty acids, fatty alcohols, and glycerol together with a small amount of aromatic compounds such as coumaric and ferulic acids (1)(2)(3)(4)(5). The distribution of the individual suberin monomeric components and compound classes varies between different plant species and parts of plants.…”

Section: Introductionmentioning

confidence: 99%

Suberin of Potato (Solanum tuberosum Var. Nikola): Comparison of the Effect of Cutinase CcCut1 with Chemical Depolymerization

Järvinen¹,

Silvestre²,

Holopainen³

et al. 2009

J. Agric. Food Chem.

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Chemical and enzymatic depolymerizations of suberin isolated from potato peel (Solanum tuberosum var. Nikola) were performed under various conditions. Enzymatic hydrolysis with cutinase CcCut1 and chemical methanolysis with NaOMe of suberin yielded monomeric fragments, which were identified as TMS derivatives with GC-MS and GC-FID. The solid, hydrolysis-resistant residues were analyzed with solid state 13 C CPMAS NMR, FT-IR, and microscopic methods. Methanolysis released more CHCl 3 -soluble material than the cutinase treatment when determined gravimetrically. Interestingly, cutinase-catalyzed hydrolysis produced higher proportions of aliphatic monomers than hydrolysis with the NaOMe procedure when analyzed by GC in the form of TMS derivatives. Monomers released by the two methods were mainly R,ω-dioic acids and ω-hydroxy acids, but the ratios of the detected monomers were different, at 40.0 and 32.7% for methanolysis and 64.6 and 8.2% for cutinase, respectively. Thus, cutinase CcCut1 showed higher activity toward ester bonds of R,ω-dioic acids than toward the bonds of ω-hydroxy acids. The most abundant monomeric compounds were octadec-9-ene-1,18-dioic acid and 18-hydroxyoctadec-9-enoic acid, which accounted for ca. 37 and 28% of all monomers, respectively. The results of the analyses of the chemical and enzymatic hydrolysis products were supported by the spectroscopic analyses with FT-IR and CPMAS 13 C NMR together with the analysis of the microstructures of the hydrolysis residues by light and confocal microscopy.

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Suberin from Plants

Abstract: Introduction Historical Outline Occurrence and Ultrastructure of Suberin Isolation of Suberized Cell Walls Chemical Composition of Suberin Aliphatic Components of Suberin Aromatic Components of Suberin Structure of Suberin Biosynth… Show more

Cited by 26 publications

References 111 publications

Apoplastic polyesters in Arabidopsis surface tissues – A typical suberin and a particular cutin

Apoplastic polyesters in Arabidopsis surface tissues – A typical suberin and a particular cutin

Apoplastic Diffusion Barriers in Arabidopsis

Suberin of Potato (Solanum tuberosum Var. Nikola): Comparison of the Effect of Cutinase CcCut1 with Chemical Depolymerization

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