Subependymal hyperintense layer on CISS sequence: An MRI study

Tsutsumi, Satoshi; Onο, Hideo; Ishii, Hisato

doi:10.1007/s00381-020-04707-2

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…37 Visualization of repetitive sequences such as centromeric, telomeric, major, and minor satellite repeats in different species (plants, mice, and humans) with RGEN-ISL have been reported. 54 Simultaneous detection of different genomic loci such as telomeres and centromeres could also be achieved with a multicolor RGEN-ISL that uses differentially labeled gRNAs. 36 As RGEN-ISL is compatible with immunostaining and 5-ethynyl-deoxyuridine (EdU) labeling, these techniques were successfully used in combination to simultaneously visualize specific DNA repeats, proteins, and DNA replication sites in fixed plant nuclei and chromosomes.…”

Section: Engineering Of Sgrnamentioning

confidence: 99%

The Current Landscape of CRISPR-Cas9-based Technologies for Imaging of Genomic Loci in Fixed and Live Cells

Yu,

Yean,

Chan

et al. 2024

Gene Expr

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Visualization of the spatiotemporal organization and dynamics of the genome in the nucleus is essential to elucidate how chromatin structure and nuclear function are intertwined. In the past, such studies were limited by the lack of appropriate tools. However, various imaging techniques now allow direct visualization of genomic loci in living cells. One of the main drivers behind this progress is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology. Initially used as an efficient and specific genome editing tool, the versatility of the CRISPR/Cas system has rapidly expanded its application range to include diagnostics, therapeutics, and, more recently, live cell imaging. In contrast to traditional fluorescent in situ hybridization, CRISPR/Cas-based imaging systems enable real-time dynamic tracking of genomic sequences down to the level of single cells and even a single locus. Recognizing the vital role played by CRISPR/ Cas-based imaging systems in advancing our understanding of genome function in relation to its spatiotemporal organization, this review aimed to depict the current landscape of CRISPR/Cas-based technologies in genomic imaging. It discusses the principles, notable features, advantages, and limitations of these CRISPR/Cas-based imaging systems and concludes with a brief outlook into the future.

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Section: Engineering Of Sgrnamentioning

confidence: 99%

The Current Landscape of CRISPR-Cas9-based Technologies for Imaging of Genomic Loci in Fixed and Live Cells

Yu,

Yean,

Chan

et al. 2024

Gene Expr

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“…Ependymal cells reside directly adjacent to the neuropil with various reports indicating the absence of a basement membrane [ 7 , 9 , 10 ]. However, basolateral labyrinths have been noted that can trap ventricular fluid and which purportedly connect to the perivascular spaces of the underlying ependymal vasculature [ 11 , 12 , 13 , 14 ]. Ependymal cells do not contain tight junctions between neighboring cells.…”

Section: The Structure and Cellular Organization Of The Ventriculamentioning

confidence: 99%

Targeting the Choroid Plexuses for Protein Drug Delivery

et al. 2020

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Delivery of therapeutic agents to the central nervous system is challenged by the barriers in place to regulate brain homeostasis. This is especially true for protein therapeutics. Targeting the barrier formed by the choroid plexuses at the interfaces of the systemic circulation and ventricular system may be a surrogate brain delivery strategy to circumvent the blood-brain barrier. Heterogenous cell populations located at the choroid plexuses provide diverse functions in regulating the exchange of material within the ventricular space. Receptor-mediated transcytosis may be a promising mechanism to deliver protein therapeutics across the tight junctions formed by choroid plexus epithelial cells. However, cerebrospinal fluid flow and other barriers formed by ependymal cells and perivascular spaces should also be considered for evaluation of protein therapeutic disposition. Various preclinical methods have been applied to delineate protein transport across the choroid plexuses, including imaging strategies, ventriculocisternal perfusions, and primary choroid plexus epithelial cell models. When used in combination with simultaneous measures of cerebrospinal fluid dynamics, they can yield important insight into pharmacokinetic properties within the brain. This review aims to provide an overview of the choroid plexuses and ventricular system to address their function as a barrier to pharmaceutical interventions and relevance for central nervous system drug delivery of protein therapeutics. Protein therapeutics targeting the ventricular system may provide new approaches in treating central nervous system diseases.

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