In this paper, we first present formulas for computing the Fréchet subdifferentials and Fréchet singular subdifferentials of the minimal time function T for a differential inclusion in R n with a general target K. These formulas are characterized in terms of Fréchet normal cones to a sub-level set of T and a level set of the minimized Hamiltonian associated with the differential inclusion. Our results extend, improve and complement to existing results. An application to sensitivity analysis for a class of time optimal control problems is also given.