Subcutaneous immunotherapy induces alterations in monocytes and dendritic cells homeostasis in allergic rhinitis patients

Sousa, L.O.; Martín-Sierra, Carmen; Pereira, Celso; Loureiro, Graça; Tavares, Beatriz; Pedreiro, Susana; Martinho, António; Paiva, Artur

doi:10.1186/s13223-018-0271-8

Cited by 7 publications

(7 citation statements)

References 52 publications

(54 reference statements)

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“…It is known that in individuals suffering from hymenoptera venom allergy, numeric and phenotypic changes in blood pDC are occurring during allergen-specific immunotherapy [ 63 ]. These findings are in line with the observation of a decrease of circulating pDC and phenotypic changes in patients with HDM allergy during subcutaneous immunotherapy [ 64 ]. Furthermore, it has been described that pDC are generally critical for tolerance induction by inducing Treg cells in a mouse model for uptake and presentation of oral allergen OVA [ 65 ].…”

Section: Discussionsupporting

confidence: 89%

Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells

Zurmühl¹,

Schmitt²,

Formentini³

et al. 2021

Clin Mol Allergy

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Background Human plasmacytoid dendritic cells (pDC) have a dual role as interferon-producing and antigen-presenting cells. Their relevance for allergic diseases is controversial. and the impact of pDC on allergic immune responses is poorly understood. Methods This in vitro study on human pDC isolated from peripheral blood was designed to compare side by side the uptake of three clinically relevant representative allergens: fluorochrome-labeled house dust mite Der p 1, Bee venom extract from Apis mellifera (Api) and the food allergen OVA analyzed flow cytometry and confocal microscopy. Results We found that the internalization and its regulation by TLR9 ligation was significantly different between allergens in terms of time course and strength of uptake. Api and OVA uptake in pDC of healthy subjects was faster and reached higher levels than Der p 1 uptake. CpG ODN 2006 suppressed OVA uptake and to a lesser extent Der p 1, while Api internalization was not affected. All allergens colocalized with LAMP1 and EEA1, with Api being internalized particularly fast and reaching highest intracellular levels in pDC. Of note, we could not determine any specific differences in antigen uptake in allergic compared with healthy subjects. Conclusions To our knowledge this is the first study that directly compares uptake regulation of clinically relevant inhalative, injective and food allergens in pDC. Our findings may help to explain differences in the onset and severity of allergic reactions as well as in the efficiency of AIT.

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Section: Discussionsupporting

confidence: 89%

Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells

Zurmühl¹,

Schmitt²,

Formentini³

et al. 2021

Clin Mol Allergy

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“…Unfortunately, in the present study, neither bilateral EVN nor SCIT increased the serum IFN-γ levels of the AR patients within 1 year after treatment. AIT is also expected to downregulate the inflammatory cytokines and chemokines [29, 30]. Our data showed that SCIT could reduce IL-4 and TNF-α levels in the peripheral blood, and these players may participate in the intrinsic mechanism of immunotherapy for AR.…”

Section: Discussionmentioning

confidence: 72%

Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

Shen¹,

Wang²,

Xue³

et al. 2021

ORL

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Background/Aims: Endoscopic vidian neurectomy (EVN) for allergic rhinitis (AR) has good clinical effects. However, the pathophysiological basis of the effect of EVN on AR is still poorly understood. This study aimed to investigate the efficacy of EVN on house dust mite (HDM)-sensitive AR and the dynamic changes of serum immunoglobulin E and some immune regulatory factors. Methods: Twenty HDM-sensitive AR patients were treated with bilateral EVN (EVN group), 15 HDM-sensitive AR patients were treated with subcutaneous immunotherapy (SCIT group), and 15 healthy subjects served as healthy controls. Quality of daily life was assessed by the scores of the Rhinoconjunctivitis Quality of Life Questionnaire (RQLQs). The visual analog scale was used to assess clinical efficacy. Serum molecules were measured by ELISA and the UNICAP system. Results: Compared with the SCIT group, the RQLQs in the EVN group were lower 12 months after treatment (both p < 0.05). There was no significant difference in improving nasal itching and sneezing (both p > 0.05), but the clinical efficacy of bilateral EVN was greater than SCIT in improving nasal obstruction, rhinorrhea, eye itching, and lachrymation 12 months after treatment (all p < 0.05). Compared with before treatment, the serum levels of total immunoglobulin E (tIgE), Dermatophagoides pteronyssinus- and Dermatophagoides farinae-specific immunoglobulin E (sIgE), and tumor necrosis factor (TNF)-α in the EVN group and the serum levels of TNF-α and interleukin-4 in the SCIT group were lower 12 months after treatment (all p < 0.05). Conclusion: The short-term efficacy of bilateral EVN is more effective than SCIT in treating HDM-sensitive AR. This may be because the surgery reduced the tIgE and sIgE levels. TNF-α may be involved in the therapeutic mechanism.

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“…One key result is the involvement of innate immune pathways associated with dendritic cells, monocytes/macrophages and mast cells. Due to their location in mucous membranes, skin, respiratory tract and blood, they encounter allergens first‐line initiating and augmenting consequent immune responses 13,52‐54 . Antigen‐presenting cells (APC), especially DCs, play a pivotal role during the sensitization and elicitation phase by propagation of Th2‐biased immune responses 55 .…”

Section: Discussionmentioning

confidence: 99%

Bet v 1 triggers antiviral‐type immune signalling in birch‐pollen‐allergic individuals

Wisgrill

Fyhrquist

Ndika

et al. 2022

Clin Experimental Allergy

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Background In allergic patients, clinical symptoms caused by pollen remind of symptoms triggered by viral respiratory infections, which are also the main cause of asthmatic exacerbations. In patients sensitized to birch pollen, Bet v 1 is the major symptom‐causing allergen. Immune mechanisms driving Bet v 1‐related responses of human blood cells have not been fully characterized. Objective To characterize the immune response to Bet v 1 in peripheral blood in patients allergic to birch pollen. Methods The peripheral blood mononuclear cells of birch‐allergic (n = 24) and non‐allergic (n = 47) adolescents were stimulated ex‐vivo followed by transcriptomic profiling. Systems‐biology approaches were employed to decipher disease‐relevant gene networks and deconvolution of associated cell populations. Results Solely in birch‐allergic patients, co‐expression analysis revealed activation of networks of innate immunity and antiviral signalling as the immediate response to Bet v 1 stimulation. Toll‐like receptors and signal transducer transcription were the main drivers of gene expression patterns. Macrophages and dendritic cells were the main cell subsets responding to Bet v 1. Conclusions and clinical relevance In birch‐pollen‐allergic patients, the activated innate immune networks seem to be, in part, the same as those activated during viral infections. This tendency of the immune system to read pollens as viruses may provide new insight to allergy prevention and treatment.

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Subcutaneous immunotherapy induces alterations in monocytes and dendritic cells homeostasis in allergic rhinitis patients

Cited by 7 publications

References 52 publications

Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells

Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells

Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

Bet v 1 triggers antiviral‐type immune signalling in birch‐pollen‐allergic individuals

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