Subcutaneous Botryomycosis Due to Bibersteinia trehalosi in a Texas Longhorn Steer

Abstract

A 3-year-old Texas Longhorn steer had a long history of progressive swelling of the soft tissues of the jaw and neck. At necropsy, multifocal to coalescing dermal and subcutaneous pyogranulomas were surrounded by fibrous tissue. Microscopically, the pyogranulomas contained aggregates of gram-negative coccobacilli surrounded by Splendore-Hoeppli material and were separated by bands of fibrovascular tissue (botryomycosis). Phylogenetic analysis of multilocus sequence-typing data revealed that the bacteria recovered in pure culture from swabs of submandibular tissue were most closely related to Bibersteinia [Pasteurella] trehalosi. The bacterial colonies were immunohistochemically reactive with a rabbit polyclonal anti-Pasteurella class C acid phosphatase antibody. Botryomycosis is a pyogranulomatous inflammation caused by a variety of nonbranching, nonfilamentous bacteria that elicit the formation of Splendore-Hoeppli material. This case of botryomycosis is unique for its association with Bibersteinia trehalosi.

Acute fibrinous pleuropneumonia and septicaemia caused byBibersteinia trehalosiin neonatal calves in New Zealand

“…An interesting case of subcutaneous botryomycosis, characterised by multifocal pyogranulomas in the soft tissues of the jaw and neck that comprised aggregates of Gram-negative coccobacilli surrounded by Splendore-Hoeppli material, has been described in a 3-year-old Texas longhorn. Although the most likely causative organism would be Actinobacillus lignieresii, the organism in this case was identified as B. trehalosi (Spagnoli et al 2012).…”

Visceral Botryomycosis by Pseudomonas aeruginosa in a bovine - case report

“…In the macroscopy, the nodular structures found, more frequently in the greater omentum, of hardened, multifocal consistency and of varying sizes, draining yellowish contents with a caseous consistency, resembled lesions commonly found in cases of intestinal tuberculosis (RIET-CORREA & GARCIA, 2007;PAES & FRANCO, 2016). However, to a lesser extent, similar lesions have been found in cases of subcutaneous, pulmonary botryomycosis, in the nasopharynx and uterus of cattle (MILLER, et al, 2001;THOMPSON et al, 2001;SPAGNOLI et al, 2011;SARTELET et al, 2015). Botryomycosis had not been raised as a possible diagnosis for the disease of this animal, since this disease presents nonspecific clinical signs and there are few descriptions in the literature, there being no reported cases of visceral botryomycosis visceral with involvement of the omentum, mesentery lymphonodes and liver of cow.…”

“…This finding corroborates with reports that described the involvement of this pathogen affecting other organs in cattle (DONAVAN;GROSS, 1984, THOMPSON et al 2001, MILLER et al 2001, SARTELET et al 2015. Some fewer common pathogens such as Escherichia coli, Proteus vulgaris, Streptococcus spp., Biberstenia trealosi may be involved (WINSLOW et al, 1959, SPAGNOLI et al, 2011.…”

“…In bovines, there are descriptions of the two forms of the disease, but the literature data are very scarce. There are reports of the cutaneous form (DONOVAN;GROSS, 1984;SPAGNOLI et al, 2011), and the visceral form in this species has been described by Thompson et al (2001), Miller et al (2001), and Sartelet et al (2015), describing a case of botryomycosis in the nasopharynx of a cow, in the lungs of a bullock, and in the uterus of cows post-surgical caesarean section, respectively (MILLER, 2001;SARTELET, 2015;THOMPSON et al, 2001).…”

Pseudomonasinfectie na keizersnede bij Belgisch witblauwe runderen

“…Bij runderen is botryomycose beschreven in de huid, onderhuid, nasofarynx en longen, met verschillende organismen als oorzaak (Donovan en Gross, 1984;Miller et al, 2001;Thompson et al, 2001;Spagnoli et al, 2012).…”