Subcutaneous Adipose Tissue Accumulation Is an Independent Risk Factor of Urinary Stone in Young People

Ye, Zixing; Xiao, He; Liu, Guanghua; Qiao, Yu; Zhao, Yi; Ji, Zhigang; Fan, Xiaohong; Li, Rongrong; Wang, Ou

doi:10.3389/fendo.2022.865930

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…However, one study reported con icting results, suggesting a stronger association between subcutaneous fat and nephrolithiasis risk 23 . Key differences in study design, including sample size, age group, and study type, may account for this discrepancy.…”

Section: Discussionmentioning

confidence: 99%

Visceral adipose tissue and nephrolithiasis risk: Evidence from National Health and Nutrition Examination Survey and Mendelian randomization analysis

2024

Preprint

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Background: This study aimed to investigate the dose‒response association and potential causal effect between VAT volume and nephrolithiasis risk. Methods: Multivariable logistic regression models were used to estimate the association between nephrolithiasis risk and VAT quartiles. Restricted cubic splines (RCSs) were employed to investigate potential nonlinear associations between visceral adipose tissue (VAT) and the likelihood of developing nephrolithiasis. A Mendelian randomization analysis was conducted to assess the causal relationship between VAT volume and nephrolithiasis risk. Results: Participants in the highest VAT quartile demonstrated a significantly greater risk of nephrolithiasis than did those in the lowest quartile across all the models: crude mode (OR [95% CI], 3.00 [1.78, 5.07]), model 1 (OR [95% CI], 2.24 [1.28, 3.92]), model 2 (OR [95% CI], 2.18 [1.24, 3.83]), and model 3 (OR [95% CI], 1.95 [0.99, 3.82]). The RCS analysis revealed a linear relationship between VAT volume and nephrolithiasis (P-nonlinear=0.443). Mendelian randomization analysis provided consistent evidence that higher VAT volume was causally associated with increased nephrolithiasis risk (OR [95% CI], 1.03 [1.02, 1.04]; P<0.001). Conclusions: This study demonstrated a positive linear causal association between VAT volume and nephrolithiasis risk.

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Section: Discussionmentioning

confidence: 99%

Visceral adipose tissue and nephrolithiasis risk: Evidence from National Health and Nutrition Examination Survey and Mendelian randomization analysis

2024

Preprint

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“…Changes in food habits and the increased incidence of obesity are the main causes of the rising prevalence of ureteral calculi [1,2]. Clinically, Medical Expulsive Therapy (MET) is beneficial due to its minimal trauma and complications.…”

Section: Introductionmentioning

confidence: 99%

PGE2 binding to EP2 promotes ureteral stone expulsion by relaxing ureter via the cAMP-PKA pathway

Su,

Zhou,

Chen

et al. 2024

BMC Urol

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Background This study investigated the relaxation effect of PGE2 on the ureter and its role in promoting calculi expulsion following calculi development. Methods By using immunofluorescence and Western blot, we were able to locate EP receptors in the ureter. In vitro experiments assessed the impact of PGE2, receptor antagonists, and agonists on ureteral relaxation rate. We constructed a model of ureteral calculi with flowable resin and collected ureteral tissue from postoperative side of the ureter after obstruction surgery. Western blot analysis was used to determine the protein expression levels of EP receptors and the PGE2 terminal synthase mPGES-1. Additionally, PGE2 was added to smooth muscle cells to observe downstream cAMP and PKA changes. Results The expression of EP2 and EP4 proteins in ureteral smooth muscle was verified by Western blot analysis. According to immunofluorescence, EP2 was primarily found on the cell membrane, while EP4 was found in the nucleus. In vitro, PGE2 induced concentration-dependent ureteral relaxation. Maximum diastolic rate was 70.94 ± 4.57% at a concentration of 30µM. EP2 antagonists hindered this effect, while EP4 antagonists did not. Obstructed ureters exhibited elevated mPGES-1 and EP2 protein expression (P < 0.01). Smooth muscle cells treated with PGE2 displayed increased cAMP and phosphorylated PKA. Conclusions PGE2 binding to EP2 induces ureteral relaxation through the cAMP-PKA pathway. This will provide a new theoretical basis for the development of new therapeutic approaches for the use of PGE2 in the treatment of ureteral stones.

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PGE2 promotes ureteral stone expulsion through ureteral relaxation via EP2 receptor

Su,

Zhou,

Chen

et al. 2023

Preprint

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This study aimed to assess the relaxant impact of PGE2 on the ureter and whether it becomes a facilitator of calculi expulsion following calculi formation. We identified the presence of EP receptors in the ureter using Western blot and immunofluorescence techniques. In vitro experiments were conducted to assess the impact of PGE2, receptor antagonists, and agonists on the rate of ureteral relaxation. Ureteral calculi models were constructed to gather ureteral tissue from the postoperative side of the obstruction. Western blot analysis was used to assess protein expression levels of EP receptors and the PGE2 terminal synthase mPGES-1. Western blot analysis revealed the presence of EP2 and EP4 protein expression in ureteral smooth muscle. Immunofluorescence revealed predominant localization of EP2 on the cell membrane and EP4 in the nucleus. In vitro experiments demonstrated that PGE2 induced concentration-dependent relaxation of the ureter. EP2 antagonists effectively inhibited the relaxation effect, while EP4 antagonists had no such inhibitory effect. In the presence of ureteral stones, the ureters on the obstructed side can become a favorable factor for stone expulsion by upregulating EP2 expression, enhancing PGE2 synthesis, and promoting ureteral relaxation via cAMP. In conclusion, PGE2 binding to EP2 induces ureteral relaxation, and in the context of ureteral calculi, it serves as a facilitator for stone expulsion.

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Subcutaneous Adipose Tissue Accumulation Is an Independent Risk Factor of Urinary Stone in Young People

Cited by 3 publications

References 27 publications

Visceral adipose tissue and nephrolithiasis risk: Evidence from National Health and Nutrition Examination Survey and Mendelian randomization analysis

Visceral adipose tissue and nephrolithiasis risk: Evidence from National Health and Nutrition Examination Survey and Mendelian randomization analysis

PGE2 binding to EP2 promotes ureteral stone expulsion by relaxing ureter via the cAMP-PKA pathway

PGE2 promotes ureteral stone expulsion through ureteral relaxation via EP2 receptor

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