Subculture Method for Large Scale Cell Culture Using Macroporous MicroCarrier

Kamiya, Kiyoshi; Yanagida, Koichiro; Shirokaze, J.

doi:10.1007/978-94-011-0437-1_120

Cited by 7 publications

(3 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cultures of cells that do not attach very well to carriers or that detach easily during mitosis can be scaled up just by diluting the microcarrier culture with fresh microcarriers and more media (275). A 1:1000 dilution has been achieved with CHO cells (Figure 86).…”

Section: Bead-to-bead Transfermentioning

confidence: 99%

Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

Wehlage¹,

Blattner²,

Mamun³

et al. 2020

AIMS Bioengineering

View full text Add to dashboard Cite

show abstract

Section: Bead-to-bead Transfermentioning

confidence: 99%

Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

Wehlage¹,

Blattner²,

Mamun³

et al. 2020

AIMS Bioengineering

View full text Add to dashboard Cite

show abstract

“…Seed microcarriers with CHO cells obtained from a 5l CelliGen reactor or 1000 ml spinner flask was transferred to the 30l Biostat UC reactor directly via tube without trypsinization. Cells could move from seed microcarriers to vacant microcarriers spontaneously (Kamiya, 1995;. As reported by Kamiya, the cell transferring speed depends on the inoculated cell density and the scale-up ratio, the higher the cell density in seed microcarriers and the lower scale-up ratio, the faster the cells move from seed microcarriers to vacant microcarriers.…”

Section: Cells Move From Seed Microcarriers To Vacant Microcarriersmentioning

confidence: 72%

Untitled

Hu¹,

Xiao²,

Huang³

et al. 2000

Cytotechnology

View full text Add to dashboard Cite

A recombinant DNA CHO cell line secretingurokinase-type plasminogen activator (u-PA) wascultivated with Cytopore cellulose porousmicrocarriers in a 30l Biostat UC stirred tankreactor. After 26 days of culture, using a spinfilter toretain cells in bioreactor, the cell density couldreach 1.33 x 10(7) ml(-1). The maximal u-PAactivity in supernatant was 7335 IU.ml(-1), and204l supernatant containing 7.1 g u-PA was harvested.After 100 days of culture with 0.1% fetal bovineserum medium, a modified cell retention system whichcan be washed-out backward, substituted thespinfilter to prevent filter clogging. The maximalcell density was over 10(7) ml(-1), the maximalu-PA activity in supernatant reached 6250IU.ml(-1), and 1604l supernatant containing about51 g u-PA was harvested. Compared to perfusionculture, batch medium-replaced culture could raiseutilizing efficiency of the medium, increase cell specificproductivity and improve the quality of the product which wasnot steady in a 37 degrees C environment. Cells can movefrom seed porous microcarriers occupied by cells tovacant microcarriers spontaneously, withouttrypsinization, and continue to grow until all microcarriers contained cells. It shows that Cytoporeporous microcarriers are very useful and convenient toscale up cultivation step by step.

show abstract

“…Just like for the cultures with Biosilon (Xiao, 1994c) and MC-1 (Xiao, 1994b) microcarriers, CL-11G cells could detach from Cytopore porous microcarriers spontaneously and reattach to fresh ones. So it was very easy to scale up the cultivation as reported by Kamiya (1995). When a scale up ratio of 3 was chosen, as shown in Figure 2, at day 18 we withdrew 2/3 of culture from a spinner flask (200 mL) and added the same volume of fresh medium and new microcarriers to the residual 1/3.…”

Section: Cells Transfer From Beads To Beadsmentioning

confidence: 99%

Untitled

Xiao¹,

Huang²,

Li³

et al. 1999

Cytotechnology

View full text Add to dashboard Cite

Using porous microcarrier Cytopore and a low-serum medium supplement BIGBEF-3, we have successfully cultivated recombinant CHO cell line CL-11G producing prourokinase and hybridomas producing anti-prourokinase monoclonal antibody in Celligen 1.5 or 5 L bioreactor. The cell density obtained ranged from 1 to 2 x 107 cells mL-1. The yields of prourokinase and monoclonal antibody increased with increasing cell density. As the cells could spontaneously release from and reattach to porous microcarriers, it was very easy to scale-up the cultivation. Thus the bead to bead cell transfer method has been used to scale up the cultivation of CL-11G cells to a 20 L reactor-scale for the pilot production of prourokinase, and also to scale-up the culture of hybridomas for the production of monoclonal antibody for the purification of prourokinase.

show abstract

Subculture Method for Large Scale Cell Culture Using Macroporous MicroCarrier

Cited by 7 publications

References 2 publications

Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

Untitled

Untitled

Contact Info

Product

Resources

About